![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, Issue 2, 421-435, February 2001
Department of Biochemistry, Molecular Biology, and Cell Biology,
Northwestern University, Evanston, Illinois 60208
Yeast Rsp5p and its mammalian homologue, Nedd4, are
hect domain ubiquitin-protein ligases (E3s) required for
the ubiquitin-dependent endocytosis of plasma membrane proteins.
Because ubiquitination is sufficient to induce internalization,
E3-mediated ubiquitination is a key regulatory event in plasma membrane
protein endocytosis. Rsp5p is an essential, multidomain protein
containing an amino-terminal C2 domain, three WW protein-protein
interaction domains, and a carboxy-terminal hect domain
that carries E3 activity. In this study, we demonstrate that Rsp5p is
peripherally associated with membranes and provide evidence that Rsp5p
functions as part of a multimeric protein complex. We define the
function of Rsp5p and its domains in the ubiquitin-dependent
internalization of the yeast 
-factor receptor, Ste2p.
Temperature-sensitive rsp5 mutants were unable to
ubiquitinate or to internalize Ste2p at the nonpermissive temperature.
Deletion of the entire C2 domain had no effect on -factor
internalization; however, point mutations in any of the three WW
domains impaired both receptor ubiquitination and internalization.
These observations indicate that the WW domains play a role in the
important regulatory event of selecting phosphorylated proteins as
endocytic cargo. In addition, mutations in the C2 and WW1 domains had
more severe defects on transport of fluid-phase markers to the vacuole
than on receptor internalization, suggesting that Rsp5p functions at
multiple steps in the endocytic pathway.
This article has been cited by other articles:
|
|
 |
|
  S. Leon, Z. Erpapazoglou, and R. Haguenauer-Tsapis Ear1p and Ssh4p Are New Adaptors of the Ubiquitin Ligase Rsp5p for Cargo Ubiquitylation and Sorting at Multivesicular Bodies Mol. Biol. Cell, June 1, 2008; 19(6): 2379 - 2388. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-Y. Chung, E. Morita, U. von Schwedler, B. Muller, H.-G. Krausslich, and W. I. Sundquist NEDD4L Overexpression Rescues the Release and Infectivity of Human Immunodeficiency Virus Type 1 Constructs Lacking PTAP and YPXL Late Domains J. Virol., May 15, 2008; 82(10): 4884 - 4897. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Haitani and H. Takagi Rsp5 is required for the nuclear export of mRNA of HSF1 and MSN2/4 under stress conditions in Saccharomyces cerevisiae. Genes Cells, February 1, 2008; 13(2): 105 - 116. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Sullivan, M. J. Lewis, E. Nikko, and H. R.B. Pelham Multiple Interactions Drive Adaptor-Mediated Recruitment of the Ubiquitin Ligase Rsp5 to Membrane Proteins In Vivo and In Vitro Mol. Biol. Cell, July 1, 2007; 18(7): 2429 - 2440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Watson and J. S. Bonifacino Direct Binding to Rsp5p Regulates Ubiquitination-independent Vacuolar Transport of Sna3p Mol. Biol. Cell, May 1, 2007; 18(5): 1781 - 1789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kim, Y. Deng, and C. C. Philpott GGA2- and Ubiquitin-dependent Trafficking of Arn1, the Ferrichrome Transporter of Saccharomyces cerevisiae Mol. Biol. Cell, May 1, 2007; 18(5): 1790 - 1802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Traub and G. L. Lukacs Decoding ubiquitin sorting signals for clathrin-dependent endocytosis by CLASPs J. Cell Sci., February 15, 2007; 120(4): 543 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Kincaid and A. A. Cooper Misfolded Proteins Traffic from the Endoplasmic Reticulum (ER) Due to ER Export Signals Mol. Biol. Cell, February 1, 2007; 18(2): 455 - 463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. W. McNatt, I. McKittrick, M. West, and G. Odorizzi Direct Binding to Rsp5 Mediates Ubiquitin-independent Sorting of Sna3 via the Multivesicular Body Pathway Mol. Biol. Cell, February 1, 2007; 18(2): 697 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Oestreich, M. Aboian, J. Lee, I. Azmi, J. Payne, R. Issaka, B. A. Davies, and D. J. Katzmann Characterization of Multiple Multivesicular Body Sorting Determinants within Sna3: A Role for the Ubiquitin Ligase Rsp5 Mol. Biol. Cell, February 1, 2007; 18(2): 707 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ren, Y. Kee, J. M. Huibregtse, and R. C. Piper Hse1, a Component of the Yeast Hrs-STAM Ubiquitin-sorting Complex, Associates with Ubiquitin Peptidases and a Ligase to Control Sorting Efficiency into Multivesicular Bodies Mol. Biol. Cell, January 1, 2007; 18(1): 324 - 335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Phelan, S. H. Millson, P. J. Parker, P. W. Piper, and F. T. Cooke Fab1p and AP-1 are required for trafficking of endogenously ubiquitylated cargoes to the vacuole lumen in S. cerevisiae J. Cell Sci., October 15, 2006; 119(20): 4225 - 4234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Staub and D. Rotin Role of Ubiquitylation in Cellular Membrane Transport Physiol Rev, April 1, 2006; 86(2): 669 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Ren, P. Vajjhala, J. S. Lee, B. Winsor, and A. L. Munn The BAR Domain Proteins: Molding Membranes in Fission, Fusion, and Phagy Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 37 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. KWAPISZ, P. CHOLBINSKI, A. K. HOPPER, J.-P. ROUSSET, and T. ZOLADEK Rsp5 ubiquitin ligase modulates translation accuracy in yeast Saccharomyces cerevisiae RNA, November 1, 2005; 11(11): 1710 - 1718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kus, A. Gajadhar, K. Stanger, R. Cho, W. Sun, N. Rouleau, T. Lee, D. Chan, C. Wolting, A. Edwards, et al. A High Throughput Screen to Identify Substrates for the Ubiquitin Ligase Rsp5 J. Biol. Chem., August 19, 2005; 280(33): 29470 - 29478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Felice, I. De Domenico, L. Li, D. M. Ward, B. Bartok, G. Musci, and J. Kaplan Post-transcriptional Regulation of the Yeast High Affinity Iron Transport System J. Biol. Chem., June 10, 2005; 280(23): 22181 - 22190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Stamenova, R. Dunn, A. S. Adler, and L. Hicke The Rsp5 Ubiquitin Ligase Binds to and Ubiquitinates Members of the Yeast CIN85-Endophilin Complex, Sla1-Rvs167 J. Biol. Chem., April 16, 2004; 279(16): 16017 - 16025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Dunn, D. A. Klos, A. S. Adler, and L. Hicke The C2 domain of the Rsp5 ubiquitin ligase binds membrane phosphoinositides and directs ubiquitination of endosomal cargo J. Cell Biol., April 12, 2004; 165(1): 135 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Angers, A. R. Ramjaun, and P. S. McPherson The HECT Domain Ligase Itch Ubiquitinates Endophilin and Localizes to the trans-Golgi Network and Endosomal System J. Biol. Chem., March 19, 2004; 279(12): 11471 - 11479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Debonneville and O. Staub Participation of the Ubiquitin-Conjugating Enzyme UBE2E3 in Nedd4-2-Dependent Regulation of the Epithelial Na+ Channel Mol. Cell. Biol., March 15, 2004; 24(6): 2397 - 2409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Katzmann, S. Sarkar, T. Chu, A. Audhya, and S. D. Emr Multivesicular Body Sorting: Ubiquitin Ligase Rsp5 Is Required for the Modification and Sorting of Carboxypeptidase S Mol. Biol. Cell, February 1, 2004; 15(2): 468 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Bouamr, J. A. Melillo, M. Q. Wang, K. Nagashima, M. de Los Santos, A. Rein, and S. P. Goff PPPYEPTAP Motif Is the Late Domain of Human T-Cell Leukemia Virus Type 1 Gag and Mediates Its Functional Interaction with Cellular Proteins Nedd4 and Tsg101 J. Virol., November 15, 2003; 77(22): 11882 - 11895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Abe and H. Iida Pressure-Induced Differential Regulation of the Two Tryptophan Permeases Tat1 and Tat2 by Ubiquitin Ligase Rsp5 and Its Binding Proteins, Bul1 and Bul2 Mol. Cell. Biol., November 1, 2003; 23(21): 7566 - 7584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Hoshikawa, M. Shichiri, S. Nakamori, and H. Takagi A nonconserved Ala401 in the yeast Rsp5 ubiquitin ligase is involved in degradation of Gap1 permease and stress-induced abnormal proteins PNAS, September 30, 2003; 100(20): 11505 - 11510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kaminska, B. Gajewska, A. K. Hopper, and T. Zoladek Rsp5p, a New Link between the Actin Cytoskeleton and Endocytosis in the Yeast Saccharomyces cerevisiae Mol. Cell. Biol., October 15, 2002; 22(20): 6946 - 6948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Traweger, D. Fang, Y.-C. Liu, W. Stelzhammer, I. A. Krizbai, F. Fresser, H.-C. Bauer, and H. Bauer The Tight Junction-specific Protein Occludin Is a Functional Target of the E3 Ubiquitin-protein Ligase Itch J. Biol. Chem., March 15, 2002; 277(12): 10201 - 10208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Tamai and C. Shimoda The novel HECT-type ubiquitin-protein ligase Pub2p shares partially overlapping function with Pub1p in Schizosaccharomyces pombe J. Cell Sci., January 5, 2002; 115(9): 1847 - 1857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Shcherbik, S. Kumar, and D. S. Haines Substrate proteolysis is inhibited by dominant-negative Nedd4 and Rsp5 mutants harboring alterations in WW domain 1 J. Cell Sci., January 3, 2002; 115(5): 1041 - 1048. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. N. Harty, M. E. Brown, J. P. McGettigan, G. Wang, H. R. Jayakar, J. M. Huibregtse, M. A. Whitt, and M. J. Schnell Rhabdoviruses and the Cellular Ubiquitin-Proteasome System: a Budding Interaction J. Virol., November 15, 2001; 75(22): 10623 - 10629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Dunn and L. Hicke Multiple Roles for Rsp5p-dependent Ubiquitination at the Internalization Step of Endocytosis J. Biol. Chem., July 6, 2001; 276(28): 25974 - 25981. [Abstract] [Full Text] [PDF]
|
|
