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Vol. 12, Issue 2, 503-510, February 2001
*Department of Physiology, University of Connecticut
Health Center, Farmington, Connecticut 06032; Nuclear envelope breakdown was investigated during meiotic
maturation of starfish oocytes. Fluorescent 70-kDa dextran entry, as
monitored by confocal microscopy, consists of two phases, a slow
uniform increase and then a massive wave. From quantitative analysis of
the first phase of dextran entry, and from imaging of green fluorescent
protein chimeras, we conclude that nuclear pore disassembly
begins several minutes before nuclear envelope breakdown. The best fit
for the second phase of entry is with a spreading disruption of the
membrane permeability barrier determined by three-dimensional computer
simulations of diffusion. We propose a new model for the mechanism of
nuclear envelope breakdown in which disassembly of the nuclear pores
leads to a fenestration of the nuclear envelope double membrane.
Howard
Hughes Medical Institute and Department of Cell Biology, Harvard
Medical School, Boston, Massachusetts 02115; and
§European Molecular Biology Laboratory, D-69117
Heidelberg, Germany
Online version of this article contains video
material for Figures 1, 2, 4, and 5. Online version available at
www.molbiolcell.org.
*
Corresponding author. E-mail address:
terasaki{at}neuron.uchc.edu.
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