Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Henneberry, A. L.
Right arrow Articles by McMaster, C. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Henneberry, A. L.
Right arrow Articles by McMaster, C. R.

Vol. 12, Issue 3, 511-520, March 2001

Phosphatidylcholine Synthesis Influences the Diacylglycerol Homeostasis Required for Sec14p-dependent Golgi Function and Cell Growth

Annette L. Henneberry, Thomas A. Lagace, Neale D. Ridgway, and Christopher R. McMaster*

The Atlantic Research Centre, Departments of Pediatrics and Biochemistry and Molecular Biology, IWK Grace Health Centre, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada

Phosphatidylcholine and phosphatidylethanolamine are the most abundant phospholipids in eukaryotic cells and thus have major roles in the formation and maintenance of vesicular membranes. In yeast, diacylglycerol accepts a phosphocholine moiety through a CPT1-derived cholinephosphotransferase activity to directly synthesize phosphatidylcholine. EPT1-derived activity can transfer either phosphocholine or phosphoethanolamine to diacylglcyerol in vitro, but is currently believed to primarily synthesize phosphatidylethanolamine in vivo. In this study we report that CPT1- and EPT1-derived cholinephosphotransferase activities can significantly overlap in vivo such that EPT1 can contribute to 60% of net phosphatidylcholine synthesis via the Kennedy pathway. Alterations in the level of diacylglycerol consumption through alterations in phosphatidylcholine synthesis directly correlated with the level of SEC14-dependent invertase secretion and affected cell viability. Administration of synthetic di8:0 diacylglycerol resulted in a partial rescue of cells from SEC14-mediated cell death. The addition of di8:0 diacylglycerol increased di8:0 diacylglycerol levels 20-40-fold over endogenous long-chain diacylglycerol levels. Di8:0 diacylglcyerol did not alter endogenous phospholipid metabolic pathways, nor was it converted to di8:0 phosphatidic acid.

* Corresponding author. E-mail address: cmcmaste{at}is.dal.ca.

Molecular Biology of the Cell
Vol. 12, 511-520, March 2001
Copyright © 2001 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 J. Cell Biol.Home page
Y. Tian, C. Pate, A. Andreolotti, L. Wang, E. Tuomanen, K. Boyd, E. Claro, and S. Jackowski
Cytokine secretion requires phosphatidylcholine synthesis
J. Cell Biol., June 16, 2008; 181(6): 945 - 957.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Pineau, L. Bonifait, J.-M. Berjeaud, P. Alimardani-Theuil, T. Berges, and T. Ferreira
A Lipid-mediated Quality Control Process in the Golgi Apparatus in Yeast
Mol. Biol. Cell, March 1, 2008; 19(3): 807 - 821.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. G. Howe, G. D. Fairn, K. MacDonald, V. A. Bankaitis, and C. R. McMaster
Regulation of Phosphoinositide Levels by the Phospholipid Transfer Protein Sec14p Controls Cdc42p/p21-Activated Kinase-Mediated Cell Cycle Progression at Cytokinesis
Eukaryot. Cell, October 1, 2007; 6(10): 1814 - 1823.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. A. Wong, G. D. Fairn, P. P. Poon, M. Shmulevitz, C. R. McMaster, R. A. Singer, and G. C. Johnston
Membrane metabolism mediated by Sec14 family members influences Arf GTPase activating protein activity for transport from the trans-Golgi
PNAS, September 6, 2005; 102(36): 12777 - 12782.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. P. Fernandez Murray and C. R. McMaster
Nte1p-mediated Deacylation of Phosphatidylcholine Functionally Interacts with Sec14p
J. Biol. Chem., March 4, 2005; 280(9): 8544 - 8552.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
S. Kagiwada and R. Zen
Role of the Yeast VAP Homolog, Scs2p, in INO1 Expression and Phospholipid Metabolism
J. Biochem., April 1, 2003; 133(4): 515 - 522.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. G. Howe, V. Zaremberg, and C. R. McMaster
Cessation of Growth to Prevent Cell Death Due to Inhibition of Phosphatidylcholine Synthesis Is Impaired at 37 {degrees}C in Saccharomyces cerevisiae
J. Biol. Chem., November 8, 2002; 277(46): 44100 - 44107.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. Zaremberg and C. R. McMaster
Differential Partitioning of Lipids Metabolized by Separate Yeast Glycerol-3-phosphate Acyltransferases Reveals That Phospholipase D Generation of Phosphatidic Acid Mediates Sensitivity to Choline-containing Lysolipids and Drugs
J. Biol. Chem., October 4, 2002; 277(41): 39035 - 39044.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. L. Henneberry, M. M. Wright, and C. R. McMaster
The Major Sites of Cellular Phospholipid Synthesis and Molecular Determinants of Fatty Acid and Lipid Head Group Specificity
Mol. Biol. Cell, September 1, 2002; 13(9): 3148 - 3161.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. A. Rudge, T. R. Pettitt, C. Zhou, M. J. O. Wakelam, and J. Engebrecht
SPO14 Separation-of-Function Mutations Define Unique Roles for Phospholipase D in Secretion and Cellular Differentiation in Saccharomyces cerevisiae
Genetics, August 1, 2001; 158(4): 1431 - 1444.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]