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Vol. 12, Issue 4, 821-830, April 2001

Mitochondrial Single-stranded DNA-binding Protein Is Required for Mitochondrial DNA Replication and Development in Drosophila melanogaster

Dieter Maier,^* Carol L. Farr, Burkhard Poeck,^* Anuradha Alahari, Marion Vogel,^* Susanne Fischer,^* Laurie S. Kaguni, and Stephan Schneuwly

 ^*Lehrstuhl für Entwicklungsbiologie, Universität Regensburg, Regensburg, Germany D-93040; and  Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824-1319

The discovery that several inherited human diseases are caused by mtDNA depletion has led to an increased interest in the replication and maintenance of mtDNA. We have isolated a new mutant in the lopo (low power) gene from Drosophila melanogaster affecting the mitochondrial single-stranded DNA-binding protein (mtSSB), which is one of the key components in mtDNA replication and maintenance. lopo¹ mutants die late in the third instar before completion of metamorphosis because of a failure in cell proliferation. Molecular, histochemical, and physiological experiments show a drastic decrease in mtDNA content that is coupled with the loss of respiration in these mutants. However, the number and morphology of mitochondria are not greatly affected. Immunocytochemical analysis shows that mtSSB is expressed in all tissues but is highly enriched in proliferating tissues and in the developing oocyte. lopo¹ is the first mtSSB mutant in higher eukaryotes, and its analysis demonstrates the essential function of this gene in development, providing an excellent model to study mitochondrial biogenesis in animals.

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Corresponding author. E-mail: Stephan.Schneuwly{at}biologie.uni-regensburg.de.

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Molecular Biology of the Cell
Vol. 12, 821-830, April 2001
Copyright © 2001 by The American Society for Cell Biology

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