Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kwak, B. R.
Right arrow Articles by Meda, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kwak, B. R.
Right arrow Articles by Meda, P.

Vol. 12, Issue 4, 831-845, April 2001

Inhibition of Endothelial Wound Repair by Dominant Negative Connexin Inhibitors

Brenda R. Kwak,*dagger || Michael S. Pepper,* Daniel B. Gros,Dagger and Paolo Meda*

 *Department of Morphology, University of Geneva Medical Center, Switzerland; and  Dagger LGPD-IBDM, Campus de Luminy, 13288 Marseille, France

Wounding of endothelial cells is associated with altered direct intercellular communication. To determine whether gap junctional communication participates to the wound repair process, we have compared connexin (Cx) expression, cell-to-cell coupling and kinetics of wound repair in monolayer cultures of PymT-transformed mouse endothelial cells (clone bEnd.3) and in bEnd.3 cells expressing different dominant negative Cx inhibitors. In parental bEnd.3 cells, mechanical wounding increased expression of Cx43 and decreased expression of Cx37 at the site of injury, whereas Cx40 expression was unaffected. These wound-induced changes in Cx expression were associated with functional changes in cell-to-cell coupling, as assessed with different fluorescent tracers. Stable transfection with cDNAs encoding for the chimeric connexin 3243H7 or the fusion protein Cx43-beta Gal resulted in perturbed gap junctional communication between bEnd.3 cells under both basal and wounded conditions. The time required for complete repair of a defined wound within a confluent monolayer was increased by ~50% in cells expressing the dominant negative Cx inhibitors, whereas other cell properties, such as proliferation rate, migration of single cells, cyst formation and extracellular proteolytic activity, were unaltered. These findings demonstrate that proper Cx expression is required for coordinated migration during repair of an endothelial wound.

dagger Corresponding author. E-mail address: brendakwak{at}hotmail.com.

|| Present address: University Hospital Geneva, Division of Cardiology, 1211 Geneva, Switzerland.

Molecular Biology of the Cell
Vol. 12, 831-845, April 2001
Copyright © 2001 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Makino, O. Platoshyn, J. Suarez, J. X.-J. Yuan, and W. H. Dillmann
Downregulation of connexin40 is associated with coronary endothelial cell dysfunction in streptozotocin-induced diabetic mice
Am J Physiol Cell Physiol, July 1, 2008; 295(1): C221 - C230.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
C. E. Chadjichristos, S. Morel, J.-P. Derouette, E. Sutter, I. Roth, A. C. Brisset, M.-L. Bochaton-Piallat, and B. R. Kwak
Targeting Connexin 43 Prevents Platelet-Derived Growth Factor-BB-Induced Phenotypic Change in Porcine Coronary Artery Smooth Muscle Cells
Circ. Res., March 28, 2008; 102(6): 653 - 660.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
R. J. Anand, S. Dai, C. Rippel, C. Leaphart, F. Qureshi, S. C. Gribar, J. W. Kohler, J. Li, D. B. Stolz, C. Sodhi, et al.
Activated macrophages inhibit enterocyte gap junctions via the release of nitric oxide
Am J Physiol Gastrointest Liver Physiol, January 1, 2008; 294(1): G109 - G119.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Lan, B. Liu, H. Yao, F. Li, T. Weng, G. Yang, W. Li, X. Cheng, N. Mao, and X. Yang
Essential Role of Endothelial Smad4 in Vascular Remodeling and Integrity
Mol. Cell. Biol., November 1, 2007; 27(21): 7683 - 7692.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
L. van Zeijl, B. Ponsioen, B. N.G. Giepmans, A. Ariaens, F. R. Postma, P. Varnai, T. Balla, N. Divecha, K. Jalink, and W. H. Moolenaar
Regulation of connexin43 gap junctional communication by phosphatidylinositol 4,5-bisphosphate
J. Cell Biol., June 21, 2007; 177(5): 881 - 891.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
Y. Liao, C. P. Regan, I. Manabe, G. K. Owens, K. H. Day, D. N. Damon, and B. R. Duling
Smooth Muscle-Targeted Knockout of Connexin43 Enhances Neointimal Formation in Response to Vascular Injury
Arterioscler. Thromb. Vasc. Biol., May 1, 2007; 27(5): 1037 - 1042.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. Mori, K. T. Power, C. M. Wang, P. Martin, and D. L. Becker
Acute downregulation of connexin43 at wound sites leads to a reduced inflammatory response, enhanced keratinocyte proliferation and wound fibroblast migration
J. Cell Sci., December 15, 2006; 119(24): 5193 - 5203.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
C. E. Chadjichristos, C. M. Matter, I. Roth, E. Sutter, G. Pelli, T. F. Luscher, M. Chanson, and B. R. Kwak
Reduced Connexin43 Expression Limits Neointima Formation After Balloon Distension Injury in Hypercholesterolemic Mice
Circulation, June 20, 2006; 113(24): 2835 - 2843.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
E. E. Ebong, S. Kim, and N. DePaola
Flow regulates intercellular communication in HAEC by assembling functional Cx40 and Cx37 gap junctional channels
Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H2015 - H2023.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. Mehta and A. B. Malik
Signaling Mechanisms Regulating Endothelial Permeability
Physiol Rev, January 1, 2006; 86(1): 279 - 367.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
T. S. Richards, C. A. Dunn, W. G. Carter, M. L. Usui, J. E. Olerud, and P. D. Lampe
Protein kinase C spatially and temporally regulates gap junctional communication during human wound repair via phosphorylation of connexin43 on serine368
J. Cell Biol., November 8, 2004; 167(3): 555 - 562.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
X. F. Figueroa, B. E. Isakson, and B. R. Duling
Connexins: Gaps in Our Knowledge of Vascular Function
Physiology, October 1, 2004; 19(5): 277 - 284.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
J.-A. Haefliger, P. Nicod, and P. Meda
Contribution of connexins to the function of the vascular wall
Cardiovasc Res, May 1, 2004; 62(2): 345 - 356.
[Abstract] [Full Text] [PDF]

Home page
 StrokeHome page
Y. Zhu, Y. Sun, L. Xie, K. Jin, N. Sheibani, and D. A. Greenberg
Hypoxic Induction of Endoglin via Mitogen-Activated Protein Kinases in Mouse Brain Microvascular Endothelial Cells
Stroke, October 1, 2003; 34(10): 2483 - 2488.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Koval
Sharing signals: connecting lung epithelial cells with gap junction channels
Am J Physiol Lung Cell Mol Physiol, November 1, 2002; 283(5): L875 - L893.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
B. R. Kwak, F. Mulhaupt, N. Veillard, D. B. Gros, and F. Mach
Altered Pattern of Vascular Connexin Expression in Atherosclerotic Plaques
Arterioscler. Thromb. Vasc. Biol., February 1, 2002; 22(2): 225 - 230.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]