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Vol. 12, Issue 4, 831-845, April 2001
and
*Department of Morphology, University of Geneva Medical Center,
Switzerland; and Wounding of endothelial cells is associated with altered direct
intercellular communication. To determine whether gap junctional communication participates to the wound repair process, we have compared connexin (Cx) expression, cell-to-cell coupling and kinetics of wound repair in monolayer cultures of PymT-transformed mouse endothelial cells (clone bEnd.3) and in bEnd.3 cells expressing different dominant negative Cx inhibitors. In parental bEnd.3 cells,
mechanical wounding increased expression of Cx43 and decreased expression of Cx37 at the site of injury, whereas Cx40 expression was
unaffected. These wound-induced changes in Cx expression were associated with functional changes in cell-to-cell coupling, as assessed with different fluorescent tracers. Stable transfection with
cDNAs encoding for the chimeric connexin 3243H7 or the fusion protein
Cx43-LGPD-IBDM, Campus de Luminy, 13288 Marseille, France
Gal resulted in perturbed gap junctional communication between
bEnd.3 cells under both basal and wounded conditions. The time required
for complete repair of a defined wound within a confluent monolayer was
increased by ~50% in cells expressing the dominant negative Cx
inhibitors, whereas other cell properties, such as proliferation rate,
migration of single cells, cyst formation and extracellular proteolytic
activity, were unaltered. These findings demonstrate that proper Cx
expression is required for coordinated migration during repair of an
endothelial wound.
Corresponding author. E-mail address:
brendakwak{at}hotmail.com.
Present address: University Hospital Geneva,
Division of Cardiology, 1211 Geneva, Switzerland.
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