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Vol. 12, Issue 6, 1595-1609, June 2001

-Catenin-independent Recruitment of ZO-1 to Nectin-based Cell-Cell Adhesion Sites through Afadin

Shigekazu Yokoyama,^* Kouichi Tachibana,^* Hiroyuki Nakanishi,^* Yasunori Yamamoto,^* Kenji Irie,^* Kenji Mandai,^* Akira Nagafuchi,^§ Morito Monden, and Yoshimi Takai^*

 ^*Department of Molecular Biology and Biochemistry and  Department of Surgery and Clinical Oncology, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita 565-0871, Japan; and  ^§Department of Cell Biology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan

ZO-1 is an actin filament (F-actin)-binding protein that localizes to tight junctions and connects claudin to the actin cytoskeleton in epithelial cells. In nonepithelial cells that have no tight junctions, ZO-1 localizes to adherens junctions (AJs) and may connect cadherin to the actin cytoskeleton indirectly through - and -catenins as one of many F-actin-binding proteins. Nectin is an immunoglobulin-like adhesion molecule that localizes to AJs and is associated with the actin cytoskeleton through afadin, an F-actin-binding protein. Ponsin is an afadin- and vinculin-binding protein that also localizes to AJs. The nectin-afadin complex has a potency to recruit the E-cadherin--catenin complex through -catenin in a manner independent of ponsin. By the use of cadherin-deficient L cell lines stably expressing various components of the cadherin-catenin and nectin-afadin systems, and -catenin-deficient F9 cell lines, we examined here whether nectin recruits ZO-1 to nectin-based cell-cell adhesion sites. Nectin showed a potency to recruit not only -catenin but also ZO-1 to nectin-based cell-cell adhesion sites. This recruitment of ZO-1 was dependent on afadin but independent of -catenin and ponsin. These results indicate that ZO-1 localizes to cadherin-based AJs through interactions not only with -catenin but also with the nectin-afadin system.

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These authors contributed equally to this work.

Corresponding author. E-mail address: ytakai{at}molbio.med.osaka-u.ac.jp.

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Molecular Biology of the Cell
Vol. 12, 1595-1609, June 2001
Copyright © 2001 by The American Society for Cell Biology

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