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Vol. 12, Issue 6, 1611-1621, June 2001

SPO21 Is Required for Meiosis-specific Modification of the Spindle Pole Body in Yeast

Bethany K. Bajgier, Maria Malzone,^* Mark Nickas, and Aaron M. Neiman

Department of Biochemistry and Cell Biology and Institute for Cell and Developmental Biology, State University of New York at Stony Brook, Stony Brook, New York 11794-5215

During meiosis II in the yeast Saccharomyces cerevisiae, the cytoplasmic face of the spindle pole body changes from a site of microtubule initiation to a site of de novo membrane formation. These membranes are required to package the haploid meiotic products into spores. This functional change in the spindle pole body involves the expansion and modification of its cytoplasmic face, termed the outer plaque. We report here that SPO21 is required for this modification. The Spo21 protein localizes to the spindle pole in meiotic cells. In the absence of SPO21 the structure of the outer plaque is abnormal, and prospore membranes do not form. Further, decreased dosage of SPO21 leaves only two of the four spindle pole bodies competent to generate membranes. Mutation of CNM67, encoding a known component of the mitotic outer plaque, also results in a meiotic outer plaque defect but does not block membrane formation, suggesting that Spo21p may play a direct role in initiating membrane formation.

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Corresponding author. E-mail address: Aaron.Neiman{at}sunysb.edu.

^* Present address: Department of Genetics, SK-50, University of Washington, Seattle, WA 98195.

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Molecular Biology of the Cell
Vol. 12, 1611-1621, June 2001
Copyright © 2001 by The American Society for Cell Biology

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