QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bajgier, B. K. Articles by Neiman, A. M. Search for Related Content PubMed PubMed Citation Articles by Bajgier, B. K. Articles by Neiman, A. M.

Vol. 12, Issue 6, 1611-1621, June 2001

SPO21 Is Required for Meiosis-specific Modification of the Spindle Pole Body in Yeast

Bethany K. Bajgier, Maria Malzone,^* Mark Nickas, and Aaron M. Neiman

Department of Biochemistry and Cell Biology and Institute for Cell and Developmental Biology, State University of New York at Stony Brook, Stony Brook, New York 11794-5215

During meiosis II in the yeast Saccharomyces cerevisiae, the cytoplasmic face of the spindle pole body changes from a site of microtubule initiation to a site of de novo membrane formation. These membranes are required to package the haploid meiotic products into spores. This functional change in the spindle pole body involves the expansion and modification of its cytoplasmic face, termed the outer plaque. We report here that SPO21 is required for this modification. The Spo21 protein localizes to the spindle pole in meiotic cells. In the absence of SPO21 the structure of the outer plaque is abnormal, and prospore membranes do not form. Further, decreased dosage of SPO21 leaves only two of the four spindle pole bodies competent to generate membranes. Mutation of CNM67, encoding a known component of the mitotic outer plaque, also results in a meiotic outer plaque defect but does not block membrane formation, suggesting that Spo21p may play a direct role in initiating membrane formation.

---

Corresponding author. E-mail address: Aaron.Neiman{at}sunysb.edu.

^* Present address: Department of Genetics, SK-50, University of Washington, Seattle, WA 98195.

---

Molecular Biology of the Cell
Vol. 12, 1611-1621, June 2001
Copyright © 2001 by The American Society for Cell Biology

This article has been cited by other articles:

				Y. Nakase, M. Nakamura-Kubo, Y. Ye, A. Hirata, C. Shimoda, and T. Nakamura Meiotic Spindle Pole Bodies Acquire the Ability to Assemble the Spore Plasma Membrane by Sequential Recruitment of Sporulation-specific Components in Fission Yeast Mol. Biol. Cell, June 1, 2008; 19(6): 2476 - 2487. [Abstract] [Full Text] [PDF]

				M. E. Pablo-Hernando, Y. Arnaiz-Pita, H. Nakanishi, D. Dawson, F. del Rey, A. M. Neiman, and C. R. V. de Aldana Cdc15 Is Required for Spore Morphogenesis Independently of Cdc14 in Saccharomyces cerevisiae Genetics, September 1, 2007; 177(1): 281 - 293. [Abstract] [Full Text] [PDF]

				H. Nakanishi, Y. Suda, and A. M. Neiman Erv14 family cargo receptors are necessary for ER exit during sporulation in Saccharomyces cerevisiae J. Cell Sci., March 1, 2007; 120(5): 908 - 916. [Abstract] [Full Text] [PDF]

				H. Nakanishi, M. Morishita, C. L. Schwartz, A. Coluccio, J. Engebrecht, and A. M. Neiman Phospholipase D and the SNARE Sso1p are necessary for vesicle fusion during sporulation in yeast. J. Cell Sci., April 1, 2006; 119(Pt 7): 1406 - 1415. [Abstract] [Full Text] [PDF]

				A. M. Neiman Ascospore Formation in the Yeast Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., December 1, 2005; 69(4): 565 - 584. [Abstract] [Full Text] [PDF]

				C. Taxis, P. Keller, Z. Kavagiou, L. J. Jensen, J. Colombelli, P. Bork, E. H.K. Stelzer, and M. Knop Spore number control and breeding in Saccharomyces cerevisiae: a key role for a self-organizing system J. Cell Biol., November 21, 2005; 171(4): 627 - 640. [Abstract] [Full Text] [PDF]

				C. G. Riedel, M. Mazza, P. Maier, R. Korner, and M. Knop Differential Requirement for Phospholipase D/Spo14 and Its Novel Interactor Sma1 for Regulation of Exocytotic Vesicle Fusion in Yeast Meiosis J. Biol. Chem., November 11, 2005; 280(45): 37846 - 37852. [Abstract] [Full Text] [PDF]

				M. A. Iwamoto, S. R. Fairclough, S. A. Rudge, and J. Engebrecht Saccharomyces cerevisiae Sps1p Regulates Trafficking of Enzymes Required for Spore Wall Synthesis Eukaryot. Cell, March 1, 2005; 4(3): 536 - 544. [Abstract] [Full Text] [PDF]

				M. E. Nickas, A. E. Diamond, M.-J. Yang, and A. M. Neiman Regulation of Spindle Pole Function by an Intermediary Metabolite Mol. Biol. Cell, June 1, 2004; 15(6): 2606 - 2616. [Abstract] [Full Text] [PDF]

				C. Shimoda Forespore membrane assembly in yeast: coordinating SPBs and membrane trafficking J. Cell Sci., January 22, 2004; 117(3): 389 - 396. [Abstract] [Full Text] [PDF]

				M. E. Nickas, C. Schwartz, and A. M. Neiman Ady4p and Spo74p Are Components of the Meiotic Spindle Pole Body That Promote Growth of the Prospore Membrane in Saccharomyces cerevisiae Eukaryot. Cell, June 1, 2003; 2(3): 431 - 445. [Abstract] [Full Text] [PDF]

				M. E. Nickas and A. M. Neiman Ady3p Links Spindle Pole Body Function to Spore Wall Synthesis in Saccharomyces cerevisiae Genetics, April 1, 2002; 160(4): 1439 - 1450. [Abstract] [Full Text] [PDF]

				T. Nakamura, M. Nakamura-Kubo, A. Hirata, and C. Shimoda The Schizosaccharomyces pombe spo3+ Gene Is Required for Assembly of the Forespore Membrane and Genetically Interacts with psy1+-encoding Syntaxin-like Protein Mol. Biol. Cell, December 1, 2001; 12(12): 3955 - 3972. [Abstract] [Full Text] [PDF]