![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, Issue 6, 1791-1799, June 2001
Howard Hughes Medical Institute and Department of Pharmacology,
University of Colorado School of Medicine, Denver, Colorado 80262
In the Xenopus oocyte system mitogen treatment
triggers the G2/M transition by transiently inhibiting the
cAMP-dependent protein kinase (PKA); subsequently, other signal
transduction pathways are activated, including the mitogen-activated
protein kinase (MAPK) and polo-like kinase pathways. To study the
interactions between these pathways, we have utilized a cell-free
oocyte extract that carries out the signaling events of oocyte
maturation after addition of the heat-stable inhibitor of PKA, PKI. PKI
stimulated the synthesis of Mos and activation of both the MAPK pathway
and the Plx1/Cdc25C/cyclin B-Cdc2 pathway. Activation of the MAPK pathway alone by glutathione S-transferase (GST)-Mos did
not lead to activation of Plx1 or cyclin B-Cdc2. Inhibition of the MAPK pathway in the extract by the MEK1 inhibitor U0126 delayed, but did not
prevent, activation of the Plx1 pathway, and inhibition of Mos
synthesis by cycloheximide had a similar effect, suggesting that MAPK
activation is the only relevant function of Mos. Immunodepletion of
Plx1 completely inhibited activation of Cdc25C and cyclin B-Cdc2 by
PKI, indicating that Plx1 is necessary for Cdc25C activation. In
extracts containing fully activated Plx1 and Cdc25C, inhibition of
cyclin B-Cdc2 by p21Cip1 had no significant effect on
either the phosphorylation of Cdc25C or the activity of Plx1. These
results demonstrate that maintenance of Plx1 and Cdc25C activity during
mitosis does not require cyclin B-Cdc2 activity.
Corresponding author. E-mail address:
Jim.Maller{at}uchsc.edu.
This article has been cited by other articles:
|
|
 |
|
  A. Seki, J. A. Coppinger, C.-Y. Jang, J. R. Yates, and G. Fang Bora and the Kinase Aurora A Cooperatively Activate the Kinase Plk1 and Control Mitotic Entry Science, June 20, 2008; 320(5883): 1655 - 1658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Budirahardja and P. Gonczy PLK-1 asymmetry contributes to asynchronous cell division of C. elegans embryos Development, April 1, 2008; 135(7): 1303 - 1313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhao, O. Haccard, R. Wang, J. Yu, J. Kuang, C. Jessus, and M. L. Goldberg Roles of Greatwall Kinase in the Regulation of Cdc25 Phosphatase Mol. Biol. Cell, April 1, 2008; 19(4): 1317 - 1327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Pal, M. T.Z. Paraz, and D. R. Kellogg Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1 J. Cell Biol., March 5, 2008; 180(5): 931 - 945. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-G. Liang, Y.-Q. Su, H.-Y. Fan, H. Schatten, and Q.-Y. Sun Mechanisms Regulating Oocyte Meiotic Resumption: Roles of Mitogen-Activated Protein Kinase Mol. Endocrinol., September 1, 2007; 21(9): 2037 - 2055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Stegmeier, M. E. Sowa, G. Nalepa, S. P. Gygi, J. W. Harper, and S. J. Elledge The tumor suppressor CYLD regulates entry into mitosis PNAS, May 22, 2007; 104(21): 8869 - 8874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Josefsberg Ben-Yehoshua, A. L. Lewellyn, P. Thomas, and J. L. Maller The Role of Xenopus Membrane Progesterone Receptor {beta} in Mediating the Effect of Progesterone on Oocyte Maturation Mol. Endocrinol., March 1, 2007; 21(3): 664 - 673. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liu, N. Jiang, J. Wu, W. Dai, and J. S. Rosenblum Polo-like Kinases Inhibited by Wortmannin: LABELING SITE AND DOWNSTREAM EFFECTS J. Biol. Chem., January 26, 2007; 282(4): 2505 - 2511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mori, Y. Yano, K. Toyo-oka, N. Yoshida, M. Yamada, M. Muramatsu, D. Zhang, H. Saya, Y. Y. Toyoshima, K. Kinoshita, et al. NDEL1 Phosphorylation by Aurora-A Kinase Is Essential for Centrosomal Maturation, Separation, and TACC3 Recruitment Mol. Cell. Biol., January 1, 2007; 27(1): 352 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Liu, M. Lei, and R. L. Erikson Normal cells, but not cancer cells, survive severe plk1 depletion. Mol. Cell. Biol., March 1, 2006; 26(6): 2093 - 2108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Zhang, L. Fletcher, and R. J. Muschel The Role of Polo-like Kinase 1 in the Inhibition of Centrosome Separation after Ionizing Radiation J. Biol. Chem., December 30, 2005; 280(52): 42994 - 42999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Liu, C.-Y. Lin, M. Lei, S. Yan, T. Zhou, and R. L. Erikson CCT Chaperonin Complex Is Required for the Biogenesis of Functional Plk1 Mol. Cell. Biol., June 15, 2005; 25(12): 4993 - 5010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Ferguson, L. S. White, P. J. Donovan, and H. Piwnica-Worms Normal Cell Cycle and Checkpoint Responses in Mice and Cells Lacking Cdc25B and Cdc25C Protein Phosphatases Mol. Cell. Biol., April 1, 2005; 25(7): 2853 - 2860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. B. Gadea and J. V. Ruderman Aurora Kinase Inhibitor ZM447439 Blocks Chromosome-induced Spindle Assembly, the Completion of Chromosome Condensation, and the Establishment of the Spindle Integrity Checkpoint in Xenopus Egg Extracts Mol. Biol. Cell, March 1, 2005; 16(3): 1305 - 1318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bachmann, H. Hennemann, P. X. Xing, I. Hoffmann, and T. Moroy The Oncogenic Serine/Threonine Kinase Pim-1 Phosphorylates and Inhibits the Activity of Cdc25C-associated Kinase 1 (C-TAK1): A NOVEL ROLE FOR Pim-1 AT THE G2/M CELL CYCLE CHECKPOINT J. Biol. Chem., November 12, 2004; 279(46): 48319 - 48328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. T. M. van Vugt, B. C. M. van de Weerdt, G. Vader, H. Janssen, J. Calafat, R. Klompmaker, R. M. F. Wolthuis, and R. H. Medema Polo-like Kinase-1 Is Required for Bipolar Spindle Formation but Is Dispensable for Anaphase Promoting Complex/Cdc20 Activation and Initiation of Cytokinesis J. Biol. Chem., August 27, 2004; 279(35): 36841 - 36854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhang, X. Shi, H. Paddon, M. Hampong, W. Dai, and S. Pelech B23/Nucleophosmin Serine 4 Phosphorylation Mediates Mitotic Functions of Polo-like Kinase 1 J. Biol. Chem., August 20, 2004; 279(34): 35726 - 35734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Erikson, T. A. J. Haystead, Y.-W. Qian, and J. L. Maller A Feedback Loop in the Polo-like Kinase Activation Pathway J. Biol. Chem., July 30, 2004; 279(31): 32219 - 32224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Di Agostino, F. Botti, A. Di Carlo, C. Sette, and R. Geremia Meiotic progression of isolated mouse spermatocytes under simulated microgravity Reproduction, July 1, 2004; 128(1): 25 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. W. Verschuren, N. Jones, and G. I. Evan The cell cycle and how it is steered by Kaposi's sarcoma-associated herpesvirus cyclin J. Gen. Virol., June 1, 2004; 85(6): 1347 - 1361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Liu, A. L. Lewellyn, L. G. Chen, and J. L. Maller The Polo Box Is Required for Multiple Functions of Plx1 in Mitosis J. Biol. Chem., May 14, 2004; 279(20): 21367 - 21373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Kellogg Wee1-dependent mechanisms required for coordination of cell growth and cell division J. Cell Sci., December 15, 2003; 116(24): 4883 - 4890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ma, J. Charron, and R. L. Erikson Role of Plk2 (Snk) in Mouse Development and Cell Proliferation Mol. Cell. Biol., October 1, 2003; 23(19): 6936 - 6943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nakajima, F. Toyoshima-Morimoto, E. Taniguchi, and E. Nishida Identification of a Consensus Motif for Plk (Polo-like Kinase) Phosphorylation Reveals Myt1 as a Plk1 Substrate J. Biol. Chem., July 3, 2003; 278(28): 25277 - 25280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. Tunquist and J. L. Maller Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs Genes & Dev., March 15, 2003; 17(6): 683 - 710. [Full Text] [PDF]
|
|
|
|
|
|
B. C. Duckworth, J. S. Weaver, and J. V. Ruderman G2 arrest in Xenopus oocytes depends on phosphorylation of cdc25 by protein kinase A PNAS, December 24, 2002; 99(26): 16794 - 16799. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Spankuch-Schmitt, J. Bereiter-Hahn, M. Kaufmann, and K. Strebhardt Effect of RNA Silencing of Polo-Like Kinase-1 (PLK1) on Apoptosis and Spindle Formation in Human Cancer Cells J Natl Cancer Inst, December 18, 2002; 94(24): 1863 - 1877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Taniguchi, F. Toyoshima-Morimoto, and E. Nishida Nuclear Translocation of Plk1 Mediated by Its Bipartite Nuclear Localization Signal J. Biol. Chem., December 6, 2002; 277(50): 48884 - 48888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. B. Deming, K. G. Flores, C. S. Downes, R. S. Paules, and W. K. Kaufmann ATR Enforces the Topoisomerase II-dependent G2 Checkpoint through Inhibition of Plk1 Kinase J. Biol. Chem., September 20, 2002; 277(39): 36832 - 36838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Kelm, M. Wind, W. D. Lehmann, and E. A. Nigg Cell Cycle-regulated Phosphorylation of the Xenopus Polo-like Kinase Plx1 J. Biol. Chem., July 5, 2002; 277(28): 25247 - 25256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Di Agostino, P. Rossi, R. Geremia, and C. Sette The MAPK pathway triggers activation of Nek2 during chromosome condensation in mouse spermatocytes Development, January 4, 2002; 129(7): 1715 - 1727. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Gross, A. L. Lewellyn, and J. L. Maller A Constitutively Active Form of the Protein Kinase p90Rsk1 Is Sufficient to Trigger the G2/M Transition in Xenopus Oocytes J. Biol. Chem., November 30, 2001; 276(49): 46099 - 46103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Machaca and S. Haun Induction of maturation-promoting factor during Xenopus oocyte maturation uncouples Ca2+ store depletion from store-operated Ca2+ entry J. Cell Biol., January 7, 2002; 156(1): 75 - 86. [Abstract] [Full Text] [PDF]
|
|
