![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, Issue 6, 1819-1833, June 2001
*Biochemie-Zentrum Heidelberg (BZH), University of Heidelberg, Im
Neuenheimer Feld 328, 69120 Heidelberg, Germany; and
Sphingomyelin- and cholesterol-enriched microdomains can be
isolated as detergent-resistant membranes from total cell extracts (total-DRM). It is generally believed that this total-DRM represents microdomains of the plasma membrane. Here we describe the purification and detailed characterization of microdomains from Golgi membranes. These Golgi-derived detergent-insoluble complexes (GICs) have a low
buoyant density and are highly enriched in lipids, containing 25% of
total Golgi phospholipids including 67% of Golgi-derived sphingomyelin, and 43% of Golgi-derived cholesterol. In contrast to
total-DRM, GICs contain only 10 major proteins, present in nearly
stoichiometric amounts, including the Max-Planck Institut für Biochemie, Martinsried,
Germany
- and 
-subunits of
heterotrimeric G proteins, flotillin-1, caveolin, and subunits of the
vacuolar ATPase. Morphological data show a brefeldin A-sensitive and
temperature-sensitive localization to the Golgi complex. Strikingly, the stability of GICs does not depend on its membrane environment, because, after addition of brefeldin A to cells, GICs can be isolated from a fused Golgi-endoplasmic reticulum organelle. This indicates that
GIC microdomains are not in a dynamic equilibrium with neighboring membrane proteins and lipids. After disruption of the microdomains by
cholesterol extraction with cyclodextrin, a subcomplex of several GIC
proteins including the B-subunit of the vacuolar ATPase,
flotillin-1, caveolin, and p17 could still be isolated by
immunoprecipitation. This indicates that several of the identified GIC
proteins localize to the same microdomains and that the microdomain
scaffold is not required for protein interactions between these GIC
proteins but instead might modulate their affinity.
Corresponding author. E-mail address:
helms{at}uni-hd.de.
This article has been cited by other articles:
|
|
 |
|
  J. Liu, H. Zhang, Z. Li, T. K. Hailemariam, M. Chakraborty, K. Jiang, D. Qiu, H. H. Bui, D. A. Peake, M.-S. Kuo, et al. Sphingomyelin Synthase 2 Is One of the Determinants for Plasma and Liver Sphingomyelin Levels in Mice Arterioscler. Thromb. Vasc. Biol., June 1, 2009; 29(6): 850 - 856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. Zaman, M. E. Resek, and S. M. Robbins Dual acylation and lipid raft association of Src-family protein tyrosine kinases are required for SDF-1/CXCL12-mediated chemotaxis in the Jurkat human T cell lymphoma cell line J. Leukoc. Biol., October 1, 2008; 84(4): 1082 - 1091. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Haberkant, O. Schmitt, F.-X. Contreras, C. Thiele, K. Hanada, H. Sprong, C. Reinhard, F. T. Wieland, and B. Brugger Protein-sphingolipid interactions within cellular membranes J. Lipid Res., January 1, 2008; 49(1): 251 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, D. Kaloyanova, M. van Eijk, R. Eerland, G. van der Goot, V. Oorschot, J. Klumperman, F. Lottspeich, V. Starkuviene, F. T. Wieland, et al. Involvement of a Golgi-resident GPI-anchored Protein in Maintenance of the Golgi Structure Mol. Biol. Cell, April 1, 2007; 18(4): 1261 - 1271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Laloi, A.-M. Perret, L. Chatre, S. Melser, C. Cantrel, M.-N. Vaultier, A. Zachowski, K. Bathany, J.-M. Schmitter, M. Vallet, et al. Insights into the Role of Specific Lipids in the Formation and Delivery of Lipid Microdomains to the Plasma Membrane of Plant Cells Plant Physiology, January 1, 2007; 143(1): 461 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. McNiven and H. M. Thompson Vesicle formation at the plasma membrane and trans-Golgi network: the same but different. Science, September 15, 2006; 313(5793): 1591 - 1594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. T. Browman, M. E. Resek, L. D. Zajchowski, and S. M. Robbins Erlin-1 and erlin-2 are novel members of the prohibitin family of proteins that define lipid-raft-like domains of the ER J. Cell Sci., August 1, 2006; 119(15): 3149 - 3160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Causeret, N. Taulet, F. Comunale, C. Favard, and C. Gauthier-Rouviere N-Cadherin Association with Lipid Rafts Regulates Its Dynamic Assembly at Cell-Cell Junctions in C2C12 Myoblasts Mol. Biol. Cell, May 1, 2005; 16(5): 2168 - 2180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Liu, S. M. DeYoung, M. Zhang, L. H. Dold, and A. R. Saltiel The Stomatin/Prohibitin/Flotillin/HflK/C Domain of Flotillin-1 Contains Distinct Sequences That Direct Plasma Membrane Localization and Protein Interactions in 3T3-L1 Adipocytes J. Biol. Chem., April 22, 2005; 280(16): 16125 - 16134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Pol, S. Martin, M. A. Fernandez, M. Ingelmo-Torres, C. Ferguson, C. Enrich, and R. G. Parton Cholesterol and Fatty Acids Regulate Dynamic Caveolin Trafficking through the Golgi Complex and between the Cell Surface and Lipid Bodies Mol. Biol. Cell, April 1, 2005; 16(4): 2091 - 2105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mielenz, C. Vettermann, M. Hampel, C. Lang, A. Avramidou, M. Karas, and H.-M. Jack Lipid Rafts Associate with Intracellular B Cell Receptors and Exhibit a B Cell Stage-Specific Protein Composition J. Immunol., March 15, 2005; 174(6): 3508 - 3517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Santamaria, E. Castellanos, V. Gomez, P. Benedit, J. Renau-Piqueras, J. Morote, J. Reventos, T. M. Thomson, and R. Paciucci PTOV1 Enables the Nuclear Translocation and Mitogenic Activity of Flotillin-1, a Major Protein of Lipid Rafts Mol. Cell. Biol., March 1, 2005; 25(5): 1900 - 1911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Crossthwaite, T. Seebacher, N. Masada, A. Ciruela, K. Dufraux, J. E. Schultz, and D. M. F. Cooper The Cytosolic Domains of Ca2+-sensitive Adenylyl Cyclases Dictate Their Targeting to Plasma Membrane Lipid Rafts J. Biol. Chem., February 25, 2005; 280(8): 6380 - 6391. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Cocucci, G. Racchetti, P. Podini, M. Rupnik, and J. Meldolesi Enlargeosome, an Exocytic Vesicle Resistant to Nonionic Detergents, Undergoes Endocytosis via a Nonacidic Route Mol. Biol. Cell, December 1, 2004; 15(12): 5356 - 5368. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Plenchette, S. Cathelin, C. Rebe, S. Launay, S. Ladoire, O. Sordet, T. Ponnelle, N. Debili, T.-H. Phan, R.-A. Padua, et al. Translocation of the inhibitor of apoptosis protein c-IAP1 from the nucleus to the Golgi in hematopoietic cells undergoing differentiation: a nuclear export signal-mediated event Blood, October 1, 2004; 104(7): 2035 - 2043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. W. Favoreel, T. C. Mettenleiter, and H. J. Nauwynck Copatching and Lipid Raft Association of Different Viral Glycoproteins Expressed on the Surfaces of Pseudorabies Virus-Infected Cells J. Virol., May 15, 2004; 78(10): 5279 - 5287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Evans, S. H. Gerber, D. Murray, and C. C. Leslie The Calcium Binding Loops of the Cytosolic Phospholipase A2 C2 Domain Specify Targeting to Golgi and ER in Live Cells Mol. Biol. Cell, January 1, 2004; 15(1): 371 - 383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Stuven, A. Porat, F. Shimron, E. Fass, D. Kaloyanova, B. Brugger, F. T. Wieland, Z. Elazar, and J. B. Helms Intra-Golgi Protein Transport Depends on a Cholesterol Balance in the Lipid Membrane J. Biol. Chem., December 26, 2003; 278(52): 53112 - 53122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Smythe, J. C. Eby, M.-H. Disatnik, and T. A. Rando A caveolin-3 mutant that causes limb girdle muscular dystrophy type 1C disrupts Src localization and activity and induces apoptosis in skeletal myotubes J. Cell Sci., December 1, 2003; 116(23): 4739 - 4749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Emery, R. G. Parton, M. Rojo, and J. Gruenberg The trans-membrane protein p25 forms highly specialized domains that regulate membrane composition and dynamics J. Cell Sci., December 1, 2003; 116(23): 4821 - 4832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Dagher, N. Donne, C. Klein, P. Ferre, and I. Dugail HDL-mediated cholesterol uptake and targeting to lipid droplets in adipocytes J. Lipid Res., October 1, 2003; 44(10): 1811 - 1820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. C. Bender, J. C. Whitbeck, M. Ponce de Leon, H. Lou, R. J. Eisenberg, and G. H. Cohen Specific Association of Glycoprotein B with Lipid Rafts during Herpes Simplex Virus Entry J. Virol., September 1, 2003; 77(17): 9542 - 9552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rodemer, T.-P. Thai, B. Brugger, T. Kaercher, H. Werner, K.-A. Nave, F. Wieland, K. Gorgas, and W. W. Just Inactivation of ether lipid biosynthesis causes male infertility, defects in eye development and optic nerve hypoplasia in mice Hum. Mol. Genet., August 1, 2003; 12(15): 1881 - 1895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Rubin and F. Ismail-Beigi Distribution of Glut1 in detergent-resistant membranes (DRMs) and non-DRM domains: effect of treatment with azide Am J Physiol Cell Physiol, August 1, 2003; 285(2): C377 - C383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hoekstra, O. Maier, J. M. van der Wouden, T. A. Slimane, and S. C. D. van IJzendoorn Membrane dynamics and cell polarity: the role of sphingolipids J. Lipid Res., May 1, 2003; 44(5): 869 - 877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. T. Shi, K.-J. Lee, H. Aizaki, S. B. Hwang, and M. M. C. Lai Hepatitis C Virus RNA Replication Occurs on a Detergent-Resistant Membrane That Cofractionates with Caveolin-2 J. Virol., April 1, 2003; 77(7): 4160 - 4168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Ratajczak, T. Damy, C. Heymes, P. Oliviero, F. Marotte, E. Robidel, R. Sercombe, J. Boczkowski, L. Rappaport, and J.-L. Samuel Caveolin-1 and -3 dissociations from caveolae to cytosol in the heart during aging and after myocardial infarction in rat Cardiovasc Res, February 1, 2003; 57(2): 358 - 369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. C. Morrow, S. Rea, S. Martin, I. A. Prior, R. Prohaska, J. F. Hancock, D. E. James, and R. G. Parton Flotillin-1/Reggie-2 Traffics to Surface Raft Domains via a Novel Golgi-independent Pathway. IDENTIFICATION OF A NOVEL MEMBRANE TARGETING DOMAIN AND A ROLE FOR PALMITOYLATION J. Biol. Chem., December 6, 2002; 277(50): 48834 - 48841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Feuk-Lagerstedt, M. Samuelsson, W. Mosgoeller, C. Movitz, A. Rosqvist, J. Bergstrom, T. Larsson, M. Steiner, R. Prohaska, and A. Karlsson The presence of stomatin in detergent-insoluble domains of neutrophil granule membranes J. Leukoc. Biol., November 1, 2002; 72(5): 970 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Engling, R. Backhaus, C. Stegmayer, C. Zehe, C. Seelenmeyer, A. Kehlenbach, B. Schwappach, S. Wegehingel, and W. Nickel Biosynthetic FGF-2 is targeted to non-lipid raft microdomains following translocation to the extracellular surface of CHO cells J. Cell Sci., September 15, 2002; 115(18): 3619 - 3631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mairhofer, M. Steiner, W. Mosgoeller, R. Prohaska, and U. Salzer Stomatin is a major lipid-raft component of platelet alpha granules Blood, July 18, 2002; 100(3): 897 - 904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. van Meer and Q. Lisman Sphingolipid Transport: Rafts and Translocators J. Biol. Chem., July 12, 2002; 277(29): 25855 - 25858. [Full Text] [PDF]
|
|
|
|
|
|
J. F. Kincer, A. Uittenbogaard, J. Dressman, T. M. Guerin, M. Febbraio, L. Guo, and E. J. Smart Hypercholesterolemia Promotes a CD36-dependent and Endothelial Nitric-oxide Synthase-mediated Vascular Dysfunction J. Biol. Chem., June 21, 2002; 277(26): 23525 - 23533. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Sotgia, B. Razani, G. Bonuccelli, W. Schubert, M. Battista, H. Lee, F. Capozza, A. L. Schubert, C. Minetti, J. T. Buckley, et al. Intracellular Retention of Glycosylphosphatidyl Inositol-Linked Proteins in Caveolin-Deficient Cells Mol. Cell. Biol., June 1, 2002; 22(11): 3905 - 3926. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. B. Eberle, R. L. Serrano, J. Fullekrug, A. Schlosser, W. D. Lehmann, F. Lottspeich, D. Kaloyanova, F. T. Wieland, and J. B. Helms Identification and characterization of a novel human plant pathogenesis-related protein that localizes to lipid-enriched microdomains in the Golgi complex J. Cell Sci., February 15, 2002; 115(4): 827 - 838. [Abstract] [Full Text] [PDF]
|
|
