![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, Issue 7, 2047-2060, July 2001
*Laboratoire de Biochimie et de Biologie Cellulaire, EA 1595, Faculté de Pharmacie, 92296 Châtenay-Malabry Cedex, France;
and We show that the Golgi complex can directly stimulate microtubule
nucleation in vivo and in vitro and thus behaves as a potent microtubule-organizing organelle in interphase cells. With the use of
nocodazole wash-out experiments in hepatic cells, we found that the
occurrence of noncentrosomal, early stabilized microtubules is highly
correlated with the subcellular localization of Golgi membranes. With
the use of in vitro reconstituted microtubule assembly systems with or
without cytosol, we also found that, in contrast to centrosomally
attached microtubules, the distal ends of Golgi-attached microtubules
are remotely stabilized in a way that requires additional cytosolic
component(s). Finally, we demonstrate that Golgi-based microtubule
nucleation is direct and involves a subset of Centre National de la Recherche Scientifique UMR 144, Institut Curie Section Recherche, 75248 Paris Cedex 05, France
-tubulin bound to the
cytoplasmic face of the organelle.
Corresponding author. E-mail address:
christian.pous{at}cep.u-psud.fr.
This article has been cited by other articles:
|
|
 |
|
  G. C. Rogers, N. M. Rusan, M. Peifer, and S. L. Rogers A Multicomponent Assembly Pathway Contributes to the Formation of Acentrosomal Microtubule Arrays in Interphase Drosophila Cells Mol. Biol. Cell, July 1, 2008; 19(7): 3163 - 3178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kodani and C. Sutterlin The Golgi Protein GM130 Regulates Centrosome Morphology and Function Mol. Biol. Cell, February 1, 2008; 19(2): 745 - 753. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hoppeler-Lebel, C. Celati, G. Bellett, M. M. Mogensen, L. Klein-Hitpass, M. Bornens, and A.-M. Tassin Centrosomal CAP350 protein stabilises microtubules associated with the Golgi complex J. Cell Sci., September 15, 2007; 120(18): 3299 - 3308. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-S. Kim, M. Takahashi, K. Matsuo, and Y. Ono Recruitment of CG-NAP to the Golgi apparatus through interaction with dynein-dynactin complex Genes Cells, March 1, 2007; 12(3): 421 - 434. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Sulimenko, E. Draberova, T. Sulimenko, L. Macurek, V. Richterova, P. Draber, and P. Draber Regulation of Microtubule Formation in Activated Mast Cells by Complexes of {gamma}-Tubulin with Fyn and Syk Kinases. J. Immunol., June 15, 2006; 176(12): 7243 - 7253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Binarova, V. Cenklova, J. Prochazkova, A. Doskocilova, J. Volc, M. Vrlik, and L. Bogre {gamma}-Tubulin Is Essential for Acentrosomal Microtubule Nucleation and Coordination of Late Mitotic Events in Arabidopsis PLANT CELL, May 1, 2006; 18(5): 1199 - 1212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Zhou, L. Cunningham, A. I. Marcus, Y. Li, and R. A. Kahn Arl2 and Arl3 Regulate Different Microtubule-dependent Processes Mol. Biol. Cell, May 1, 2006; 17(5): 2476 - 2487. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Hollande, C. Salvador-Cartier, L. Alvarez, and M. Fanjul Expression of a Wild-type CFTR Maintains the Integrity of the Biosynthetic/Secretory Pathway in Human Cystic Fibrosis Pancreatic Duct Cells J. Histochem. Cytochem., December 1, 2005; 53(12): 1539 - 1552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sutterlin, R. Polishchuk, M. Pecot, and V. Malhotra The Golgi-associated Protein GRASP65 Regulates Spindle Dynamics and Is Essential for Cell Division Mol. Biol. Cell, July 1, 2005; 16(7): 3211 - 3222. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Janson, T. G. Setty, A. Paoletti, and P.T. Tran Efficient formation of bipolar microtubule bundles requires microtubule-bound {gamma}-tubulin complexes J. Cell Biol., April 25, 2005; 169(2): 297 - 308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. Barr and J. Egerer Golgi positioning: are we looking at the right MAP? J. Cell Biol., March 28, 2005; 168(7): 993 - 998. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Jouvenet and T. Wileman African swine fever virus infection disrupts centrosome assembly and function J. Gen. Virol., March 1, 2005; 86(3): 589 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. BALUSKA, D. VOLKMANN, and P. W. BARLOW Eukaryotic Cells and their Cell Bodies: Cell Theory Revised Ann. Bot., July 1, 2004; 94(1): 9 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Merlot, R. Meili, D. J. Pagliarini, T. Maehama, J. E. Dixon, and R. A. Firtel A PTEN-related 5-Phosphatidylinositol Phosphatase Localized in the Golgi J. Biol. Chem., October 10, 2003; 278(41): 39866 - 39873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Magdalena, T. H. Millard, and L. M. Machesky Microtubule involvement in NIH 3T3 Golgi and MTOC polarity establishment J. Cell Sci., February 15, 2003; 116(4): 743 - 756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Drykova, V. Cenklova, V. Sulimenko, J. Volc, P. Draber, and P. Binarova Plant {gamma}-Tubulin Interacts with {alpha}{beta}-Tubulin Dimers and Forms Membrane-Associated Complexes PLANT CELL, February 1, 2003; 15(2): 465 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Straube, M. Brill, B. R. Oakley, T. Horio, and G. Steinberg Microtubule Organization Requires Cell Cycle-dependent Nucleation at Dispersed Cytoplasmic Sites: Polar and Perinuclear Microtubule Organizing Centers in the Plant Pathogen Ustilago maydis Mol. Biol. Cell, February 1, 2003; 14(2): 642 - 657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wang and W. Hong Interorganellar Regulation of Lysosome Positioning by the Golgi Apparatus through Rab34 Interaction with Rab-interacting Lysosomal Protein Mol. Biol. Cell, December 1, 2002; 13(12): 4317 - 4332. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Carre, N. Andre, G. Carles, H. Borghi, L. Brichese, C. Briand, and D. Braguer Tubulin Is an Inherent Component of Mitochondrial Membranes That Interacts with the Voltage-dependent Anion Channel J. Biol. Chem., September 6, 2002; 277(37): 33664 - 33669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. C. Steveson, G. C. Zhao, H. T. Keutmann, R. E. Mains, and B. A. Eipper Access of a Membrane Protein to Secretory Granules Is Facilitated by Phosphorylation J. Biol. Chem., October 19, 2001; 276(43): 40326 - 40337. [Abstract] [Full Text] [PDF]
|
|
