Molecular Biology of the Cell click for CBE Life Science Education Page

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kondylis, V.
Right arrow Articles by Rabouille, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kondylis, V.
Right arrow Articles by Rabouille, C.

Vol. 12, Issue 8, 2308-2327, August 2001

Biogenesis of Golgi Stacks in Imaginal Discs of Drosophila melanogaster

Vangelis Kondylis, Sarah E. Goulding, Jonathan C. Dunne, and Catherine Rabouille*

The Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh, EH9 3JR, Scotland, United Kingdom

We provide a detailed description of Golgi stack biogenesis that takes place in vivo during one of the morphogenetic events in the lifespan of Drosophila melanogaster. In early third-instar larvae, small clusters consisting mostly of vesicles and tubules were present in epithelial imaginal disk cells. As larvae progressed through mid- and late-third instar, these larval clusters became larger but also increasingly formed cisternae, some of which were stacked. In white pupae, the typical Golgi stack was observed. We show that larval clusters are Golgi stack precursors by 1) localizing various Golgi-specific markers to the larval clusters by electron and immunofluorescence confocal microscopy, 2) driving this conversion in wild-type larvae incubated at 37°C for 2 h, and 3) showing that this conversion does not take place in an NSF1 mutant (comt 17). The biological significance of this conversion became clear when we found that the steroid hormone 20-hydroxyecdysone (ecdysone) is critically involved in this conversion. In its absence, Golgi stack biogenesis did not occur and the larval clusters remained unaltered. We showed that dGM130 and sec23p expression increases approximately three- and fivefold, respectively, when discs are exposed to ecdysone in vivo and in vitro. Taken together, these results suggest that we have developed an in vivo system to study the ecdysone-triggered Golgi stack biogenesis.

* Corresponding author. E-mail address: C.Rabouille{at}ed.ac.uk.

Molecular Biology of the Cell
Vol. 12, 2308-2327, August 2001
Copyright © 2001 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
S. Kametaka, N. Sawada, J. S. Bonifacino, and S. Waguri
Functional characterization of protein-sorting machineries at the trans-Golgi network in Drosophila melanogaster
J. Cell Sci., February 1, 2010; 123(3): 460 - 471.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Schotman, L. Karhinen, and C. Rabouille
Integrins mediate their unconventional, mechanical-stress-induced secretion via RhoA and PINCH in Drosophila
J. Cell Sci., August 1, 2009; 122(15): 2662 - 2672.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
Z. Jin, E. Homola, S. Tiong, and S. D. Campbell
Drosophila Myt1 Is the Major Cdk1 Inhibitory Kinase for Wing Imaginal Disc Development
Genetics, December 1, 2008; 180(4): 2123 - 2133.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Januschke, E. Nicolas, J. Compagnon, E. Formstecher, B. Goud, and A. Guichet
Rab6 and the secretory pathway affect oocyte polarity in Drosophila
Development, October 1, 2007; 134(19): 3419 - 3425.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J.-B. Coutelis and A. Ephrussi
Rab6 mediates membrane organization and determinant localization during Drosophila oogenesis
Development, April 1, 2007; 134(7): 1419 - 1430.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. C. Eisman, N. Stewart, D. Miller, and T. C. Kaufman
centrosomin's beautiful sister (cbs) encodes a GRIP-domain protein that marks Golgi inheritance and functions in the centrosome cycle inDrosophila
J. Cell Sci., August 15, 2006; 119(16): 3399 - 3412.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Yano, M. Yamamoto-Hino, M. Abe, R. Kuwahara, S. Haraguchi, I. Kusaka, W. Awano, A. Kinoshita-Toyoda, H. Toyoda, and S. Goto
Distinct functional units of the Golgi complex in Drosophila cells
PNAS, September 20, 2005; 102(38): 13467 - 13472.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
V. Kondylis, K. M. Spoorendonk, and C. Rabouille
dGRASP Localization and Function in the Early Exocytic Pathway in Drosophila S2 Cells
Mol. Biol. Cell, September 1, 2005; 16(9): 4061 - 4072.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
V. Kondylis and C. Rabouille
A novel role for dp115 in the organization of tER sites in Drosophila
J. Cell Biol., July 21, 2003; 162(2): 185 - 198.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]