![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, Issue 8, 2469-2481, August 2001
-Tubulin Reveal Its Involvement in
Chromosome Segregation and Cytokinesis
and
Program in Biochemistry, Cell, and Developmental Biology,
*Departments of Cell Biology and Biochemistry, Emory
University School of Medicine, Atlanta, Georgia 30322
-Tubulin is a conserved essential protein required for assembly
and function of the mitotic spindle in humans and yeast. For example,
human -tubulin can replace the -tubulin gene in Schizosaccharomyces pombe. To understand the
structural/functional domains of -tubulin, we performed a systematic
alanine-scanning mutagenesis of human -tubulin
(TUBG1) and studied phenotypes of each mutant allele in
S. pombe. Our screen, both in the presence and absence
of the endogenous S. pombe -tubulin, resulted in 11 lethal mutations and 12 cold-sensitive mutations. Based on structural
mapping onto a homology model of human -tubulin generated by free
energy minimization, all deleterious mutations are found in residues
predicted to be located on the surface, some in positions to interact
with 
- and/or 
-tubulins in the microtubule lattice. As expected,
one class of tubg1 mutations has either an abnormal assembly or loss of the mitotic spindle. Surprisingly, a subset of
mutants with abnormal spindles does not arrest in M phase but proceeds
through anaphase followed by abnormal cytokinesis. These studies reveal
that in addition to its previously appreciated role in spindle
microtubule nucleation, -tubulin is involved in the coordination of
postmetaphase events, anaphase, and cytokinesis.
These authors contributed equally to this work.
§
Corresponding author. E-mail address:
joshi{at}cellbio.emory.edu.
This article has been cited by other articles:
|
|
 |
|
  C. Wiese Distinct Dgrip84 Isoforms Correlate with Distinct {gamma}-Tubulins in Drosophila Mol. Biol. Cell, January 1, 2008; 19(1): 368 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. A. Eckardt Function of {gamma}-Tubulin in Plants PLANT CELL, June 1, 2006; 18(6): 1327 - 1329. [Full Text] [PDF]
|
|
|
|
|
|
M. Pastuglia, J. Azimzadeh, M. Goussot, C. Camilleri, K. Belcram, J.-L. Evrard, A.-C. Schmit, P. Guerche, and D. Bouchez {gamma}-Tubulin Is Essential for Microtubule Organization and Development in Arabidopsis PLANT CELL, June 1, 2006; 18(6): 1412 - 1425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Binarova, V. Cenklova, J. Prochazkova, A. Doskocilova, J. Volc, M. Vrlik, and L. Bogre {gamma}-Tubulin Is Essential for Acentrosomal Microtubule Nucleation and Coordination of Late Mitotic Events in Arabidopsis PLANT CELL, May 1, 2006; 18(5): 1199 - 1212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Masuda, T. Toda, R. Miyamoto, T. Haraguchi, and Y. Hiraoka Modulation of Alp4 function in Schizosaccharomyces pombe induces novel phenotypes that imply distinct functions for nuclear and cytoplasmic {gamma}-tubulin complexes Genes Cells, April 1, 2006; 11(4): 319 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Colombie, C. Verollet, P. Sampaio, A. Moisand, C. Sunkel, H.-M. Bourbon, M. Wright, and B. Raynaud-Messina The Drosophila {gamma}-Tubulin Small Complex Subunit Dgrip84 Is Required for Structural and Functional Integrity of the Spindle Apparatus Mol. Biol. Cell, January 1, 2006; 17(1): 272 - 282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shang, C.-C. Tsao, and M. A. Gorovsky Mutational analyses reveal a novel function of the nucleotide-binding domain of {gamma}-tubulin in the regulation of basal body biogenesis J. Cell Biol., December 19, 2005; 171(6): 1035 - 1044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sankaran, L. M. Starita, A. C. Groen, M. J. Ko, and J. D. Parvin Centrosomal Microtubule Nucleation Activity Is Inhibited by BRCA1-Dependent Ubiquitination Mol. Cell. Biol., October 1, 2005; 25(19): 8656 - 8668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zimmerman and F. Chang Effects of {gamma}-Tubulin Complex Proteins on Microtubule Nucleation and Catastrophe in Fission Yeast Mol. Biol. Cell, June 1, 2005; 16(6): 2719 - 2733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tange, A. Fujita, T. Toda, and O. Niwa Functional Dissection of the {gamma}-Tubulin Complex by Suppressor Analysis of gtb1 and alp4 Mutations in Schizosaccharomyces pombe Genetics, July 1, 2004; 167(3): 1095 - 1107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Prigozhina, C. E. Oakley, A. M. Lewis, T. Nayak, S. A. Osmani, and B. R. Oakley {gamma}-Tubulin Plays an Essential Role in the Coordination of Mitotic Events Mol. Biol. Cell, March 1, 2004; 15(3): 1374 - 1386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Drykova, V. Cenklova, V. Sulimenko, J. Volc, P. Draber, and P. Binarova Plant {gamma}-Tubulin Interacts with {alpha}{beta}-Tubulin Dimers and Forms Membrane-Associated Complexes PLANT CELL, February 1, 2003; 15(2): 465 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shang, B. Li, and M. A. Gorovsky Tetrahymena thermophila contains a conventional {gamma}-tubulin that is differentially required for the maintenance of different microtubule-organizing centers J. Cell Biol., September 29, 2002; 158(7): 1195 - 1206. [Full Text] [PDF]
|
|
|
|
|
|
A. Fujita, L. Vardy, M. A. Garcia, and T. Toda A Fourth Component of the Fission Yeast gamma -Tubulin Complex, Alp16, Is Required for Cytoplasmic Microtubule Integrity and Becomes Indispensable When gamma -Tubulin Function Is Compromised Mol. Biol. Cell, July 1, 2002; 13(7): 2360 - 2373. [Abstract] [Full Text] [PDF]
|
|
