![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 12, Issue 8, 2482-2496, August 2001
*Consiglio Nazionale delle Ricerche, Cellular and Molecular
Pharmacology Center and Department of Medical Pharmacology, University
of Milan, Milan, Italy; and Many mitochondrial outer membrane (MOM) proteins have a
transmembrane domain near the C terminus and an N-terminal cytosolic moiety. It is not clear how these tail-anchored (TA) proteins posttranslationally select their target, but C-terminal charged residues play an important role. To investigate how discrimination between MOM and endoplasmic reticulum (ER) occurs, we used mammalian cytochrome b5, a TA protein existing in two,
MOM or ER localized, versions. Substitution of the seven C-terminal
residues of the ER isoform or of green fluorescent protein reporter
constructs with one or two arginines resulted in MOM-targeted proteins,
whereas a single C-terminal threonine caused promiscuous localization. To investigate whether targeting to MOM occurs from the cytosol or
after transit through the ER, we tagged a MOM-directed construct with a
C-terminal N-glycosylation sequence. Although in vitro this construct was efficiently glycosylated by microsomes, the protein
expressed in vivo localized almost exclusively to MOM, and was nearly
completely unglycosylated. The small fraction of glycosylated protein
was in the ER and was not a precursor to the unglycosylated form. Thus,
targeting occurs directly from the cytosol. Moreover, ER and MOM
compete for the same polypeptide, explaining the dual localization of
some TA proteins.
Faculty of Pharmacy,
University of Catanzaro "Magna Graecia," Catanzaro, Italy
Corresponding author. E-mail address:
Nica{at}csfic.mi.cnr.it.
This article has been cited by other articles:
|
|
 |
|
  C. Kemper, S. J. Habib, G. Engl, P. Heckmeyer, K. S. Dimmer, and D. Rapaport Integration of tail-anchored proteins into the mitochondrial outer membrane does not require any known import components J. Cell Sci., June 15, 2008; 121(12): 1990 - 1998. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ma and S. S. Taylor A Molecular Switch for Targeting between Endoplasmic Reticulum (ER) and Mitochondria: CONVERSION OF A MITOCHONDRIA-TARGETING ELEMENT INTO AN ER-TARGETING SIGNAL IN DAKAP1 J. Biol. Chem., April 25, 2008; 283(17): 11743 - 11751. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Bozidis, C. D. Williamson, and A. M. Colberg-Poley Mitochondrial and Secretory Human Cytomegalovirus UL37 Proteins Traffic into Mitochondrion-Associated Membranes of Human Cells J. Virol., March 15, 2008; 82(6): 2715 - 2726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Abell, C. Rabu, P. Leznicki, J. C. Young, and S. High Post-translational integration of tail-anchored proteins is facilitated by defined molecular chaperones J. Cell Sci., May 15, 2007; 120(10): 1743 - 1751. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Maggio, A. Barbante, F. Ferro, L. Frigerio, and E. Pedrazzini Intracellular sorting of the tail-anchored protein cytochrome b5 in plants: a comparative study using different isoforms from rabbit and Arabidopsis J. Exp. Bot., April 1, 2007; 58(6): 1365 - 1379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Brambillasca, M. Yabal, M. Makarow, and N. Borgese Unassisted translocation of large polypeptide domains across phospholipid bilayers J. Cell Biol., December 4, 2006; 175(5): 767 - 777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Taghbalout, L. Ma, and L. Rothfield Role Of MinD-Membrane Association in Min Protein Interactions. J. Bacteriol., April 1, 2006; 188(8): 2993 - 3001. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. M. A. van Herpen, R. J. A. Oude Ophuis, M. Wijers, M. B. Bennink, F. A. J. van de Loo, J. Fransen, B. Wieringa, and D. G. Wansink Divergent Mitochondrial and Endoplasmic Reticulum Association of DMPK Splice Isoforms Depends on Unique Sequence Arrangements in Tail Anchors Mol. Cell. Biol., February 15, 2005; 25(4): 1402 - 1414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. T. Hwang, S. M. Pelitire, M. P.A. Henderson, D. W. Andrews, J. M. Dyer, and R. T. Mullen Novel Targeting Signals Mediate the Sorting of Different Isoforms of the Tail-Anchored Membrane Protein Cytochrome b5 to Either Endoplasmic Reticulum or Mitochondria PLANT CELL, November 1, 2004; 16(11): 3002 - 3019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. de Avalos, Y. Okamoto, and Y. A. Hannun Activation and Localization of Inositol Phosphosphingolipid Phospholipase C, Isc1p, to the Mitochondria during Growth of Saccharomyces cerevisiae J. Biol. Chem., March 19, 2004; 279(12): 11537 - 11545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Guillou, S. D'Andrea, V. Rioux, R. Barnouin, S. Dalaine, F. Pedrono, S. Jan, and P. Legrand Distinct roles of endoplasmic reticulum cytochrome b5 and fused cytochrome b5-like domain for rat {Delta}6-desaturase activity J. Lipid Res., January 1, 2004; 45(1): 32 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Snapp, R. S. Hegde, M. Francolini, F. Lombardo, S. Colombo, E. Pedrazzini, N. Borgese, and J. Lippincott-Schwartz Formation of stacked ER cisternae by low affinity protein interactions J. Cell Biol., October 27, 2003; 163(2): 257 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Horie, H. Suzuki, M. Sakaguchi, and K. Mihara Targeting and Assembly of Mitochondrial Tail-anchored Protein Tom5 to the TOM Complex Depend on a Signal Distinct from That of Tail-anchored Proteins Dispersed in the Membrane J. Biol. Chem., October 17, 2003; 278(42): 41462 - 41471. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Geuze, J. L. Murk, A. K. Stroobants, J. M. Griffith, M. J. Kleijmeer, A. J. Koster, A. J. Verkleij, B. Distel, and H. F. Tabak Involvement of the Endoplasmic Reticulum in Peroxisome Formation Mol. Biol. Cell, July 1, 2003; 14(7): 2900 - 2907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Borgese, S. Colombo, and E. Pedrazzini The tale of tail-anchored proteins: coming from the cytosol and looking for a membrane J. Cell Biol., June 23, 2003; 161(6): 1013 - 1019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Germain and G. C. Shore Cellular Distribution of Bcl-2 Family Proteins Sci. Signal., March 11, 2003; 2003(173): pe10 - pe10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Beilharz, B. Egan, P. A. Silver, K. Hofmann, and T. Lithgow Bipartite Signals Mediate Subcellular Targeting of Tail-anchored Membrane Proteins in Saccharomyces cerevisiae J. Biol. Chem., February 28, 2003; 278(10): 8219 - 8223. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Abell, M. Jung, J. D. Oliver, B. C. Knight, J. Tyedmers, R. Zimmermann, and S. High Tail-anchored and Signal-anchored Proteins Utilize Overlapping Pathways during Membrane Insertion J. Biol. Chem., February 14, 2003; 278(8): 5669 - 5678. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yabal, S. Brambillasca, P. Soffientini, E. Pedrazzini, N. Borgese, and M. Makarow Translocation of the C Terminus of a Tail-anchored Protein across the Endoplasmic Reticulum Membrane in Yeast Mutants Defective in Signal Peptide-driven Translocation J. Biol. Chem., January 24, 2003; 278(5): 3489 - 3496. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Horie, H. Suzuki, M. Sakaguchi, and K. Mihara Characterization of Signal That Directs C-Tail-anchored Proteins to Mammalian Mitochondrial Outer Membrane Mol. Biol. Cell, May 1, 2002; 13(5): 1615 - 1625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rojo, F. Legros, D. Chateau, and A. Lombes Membrane topology and mitochondrial targeting of mitofusins, ubiquitous mammalian homologs of the transmembrane GTPase Fzo J. Cell Sci., April 15, 2002; 115(8): 1663 - 1674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bulbarelli, T. Sprocati, M. Barberi, E. Pedrazzini, and N. Borgese Trafficking of tail-anchored proteins: transport from the endoplasmic reticulum to the plasma membrane and sorting between surface domains in polarised epithelial cells J. Cell Sci., April 15, 2002; 115(8): 1689 - 1702. [Abstract] [Full Text] [PDF]
|
|
