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Vol. 12, Issue 8, 2534-2545, August 2001
and
Actin microfilaments, which are prominent in pollen tubes, have
been implicated in the growth process; however, their mechanism of
action is not well understood. In the present work we have used
profilin and DNAse I injections, as well as latrunculin B and
cytochalasin D treatments, under quantitatively controlled conditions,
to perturb actin microfilament structure and assembly in an attempt to
answer this question. We found that a ~50% increase in the total
profilin pool was necessary to half-maximally inhibit pollen tube
growth, whereas a ~100% increase was necessary for half-maximal
inhibition of cytoplasmic streaming. DNAse I showed a similar
inhibitory activity but with a threefold more pronounced effect on
growth than streaming. Latrunculin B, at only 1-4 nM in the growth
medium, has a similar proportion of inhibition of growth over
streaming to that of profilin. The fact that tip growth is more
sensitive than streaming to the inhibitory substances and that there is
no correlation between streaming and growth rates suggests that tip
growth requires actin assembly in a process independent of cytoplasmic streaming.
Biology Department, Molecular and Cellular
Biology Program, University of Massachusetts, Amherst, Massachusetts
01003; Biology Department, Long Island University,
Brooklyn, New York 11201
Online version of this article contains video material for
Figures 7 and 9. Online version is available at www.molbiolcell.org.
*
Corresponding author. E-mail address:
lvidali{at}bio.umass.edu.
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