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Vol. 12, Issue 9, 2590-2600, September 2001
and
*Division of Biology, Cell-type specific genes were recognized by interrogating
microarrays carrying Dictyostelium gene fragments with
probes prepared from fractions enriched in prestalk and prespore
cells. Cell-type specific accumulation of mRNA from 17 newly
identified genes was confirmed by Northern analyses. DNA microarrays
carrying 690 targets were used to determine expression profiles during
development. The profiles were fit to a biologically based kinetic
equation to extract the times of transcription onset and cessation.
Although the majority of the genes that were cell-type enriched at the slug stage were first expressed as the prespore and prestalk cells sorted out in aggregates, some were found to be expressed earlier before the cells had even aggregated. These early genes may have been
initially expressed in all cells and then preferentially turned over in
one or the other cell type. Alternatively, cell type divergence may
start soon after the initiation of development.
Department of Physics,
University of California at San Diego, La Jolla, California 92093; and
Department of Cell and Developmental Biology, Stanford
Medical School, Stanford, California 94305
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