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Vol. 12, Issue 9, 2688-2698, September 2001
Max-Planck-Institute for Evolutionary Anthropology, D-04105
Leipzig, Germany
The mitochondrial tRNA gene for lysine was analyzed in 11 different
marsupial mammals. Whereas its location is conserved when compared with
other vertebrate mitochondrial genomes, its primary sequence and
inferred secondary structure are highly unusual and variable. For
example, eight species lack the expected anticodon. Because the
corresponding transcripts are not altered by any RNA-editing mechanism,
the lysyl-tRNA gene seems to represent a mitochondrial pseudogene.
Purification of marsupial mitochondria and in vitro aminoacylation of
isolated tRNAs with lysine, followed by analysis of aminoacylated
tRNAs, show that a nuclear-encoded tRNALys is associated
with marsupial mitochondria. We conclude that a functional
tRNALys encoded in the nuclear genome is imported into
mitochondria in marsupials. Thus, tRNA import is not restricted to
plant, yeast, and protozoan mitochondria but also occurs also in mammals.
GSF-Haematologikum, Institut für Klinische
Molekularbiologie, Marchioninistrasse 25, 81377 Munich, Germany.
Corresponding author. E-mail address:
moerl{at}eva.mpg.de.
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