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Vol. 12, Issue 9, 2767-2775, September 2001
*Karolinska Institutet, Department of Biosciences Novum/University
College Sodertorn, Department of Natural Sciences, S-141 04 Huddinge,
Sweden; The centromeric DNA of fission yeast is arranged with a central
core flanked by repeated sequences. The centromere-associated proteins,
Mis6p and Cnp1p (SpCENP-A), associate exclusively with central core
DNA, whereas the Swi6 protein binds the surrounding repeats. Here,
electron microscopy and immunofluorescence light microscopy reveal that
the central core and flanking regions occupy distinct positions within
a heterochromatic domain. An "anchor" structure containing the
Ndc80 protein resides between this heterochromatic domain and the
spindle pole body. The organization of centromere-associated proteins
in fission yeast is reminiscent of the multilayered structures of human
kinetochores, indicating that such domain structure is conserved in eukaryotes.
3-D Laboratory for Fine Structure, University of
Colorado, Boulder, Colorado 80309; Medical Research
Council Human Genetics Unit, Edinburgh, United Kingdom EH4 2XU; and
§Huddinge Sjukhus, Clinical Research Center, Electron
Microscopy Unit, S-141 86 Huddinge, Sweden
Corresponding author. E-mail address:
karl.ekwall{at}cbt.ki.se.
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