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Vol. 12, Issue 9, 2858-2869, September 2001

MAP-1 and IAP-1, Two Novel AAA Proteases with Catalytic Sites on Opposite Membrane Surfaces in Mitochondrial Inner Membrane of Neurospora crassa

Carola Klanner,^* Holger Prokisch,^* and Thomas Langer^§

 ^*Institut für Physiologische Chemie, Universität München, 81377 München, Germany; and  Institut für Genetik, Universität zu Köln, 50674 Köln, Germany

Eukaryotic AAA proteases form a conserved family of membrane-embedded ATP-dependent proteases but have been analyzed functionally only in the yeast Saccharomyces cerevisiae. Here, we have identified two novel members of this protein family in the filamentous fungus Neurospora crassa, which were termed MAP-1 and IAP-1. Both proteins are localized to the inner membrane of mitochondria. They are part of two similar-sized high molecular mass complexes, but expose their catalytic sites to opposite membrane surfaces, namely, the intermembrane and the matrix space. Disruption of iap-1 by repeat-induced point mutation caused a slow growth phenotype at high temperature and stabilization of a misfolded inner membrane protein against degradation. IAP-1 could partially substitute for functions of its yeast homolog Yme1, demonstrating functional conservation. However, respiratory growth at 37°C was not restored. Our results identify two components of the quality control system of the mitochondrial inner membrane in N. crassa and suggest that AAA proteases with catalytic sites exposed to opposite membrane surfaces are present in mitochondria of all eukaryotic cells.

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These authors contributed equally to this work.

^§ Corresponding author. E-mail address: Thomas.Langer{at}uni-koeln.de.

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Molecular Biology of the Cell
Vol. 12, 2858-2869, September 2001
Copyright © 2001 by The American Society for Cell Biology

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