![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 13, Issue 1, 119-133, January 2002
Department of Cell Biology, University of
Alberta, Edmonton, Alberta T6G 2H7, Canada
Activation of several ADP-ribosylation factors (ARFs)
by guanine nucleotide exchange factors (GEFs) regulates
recruitment of coat proteins (COPs) on the Golgi complex and
is generally assumed to be the target of brefeldin A (BFA).
The large ARF-GEFs Golgi-specific BFA resistance factor 1 (GBF1) and BFA-inhibited GEFs (BIGs) localize to this
organelle but catalyze exchange preferentially on class II
and class I ARFs, respectively. We now demonstrate using
quantitative confocal microscopy that these GEFs show a very
limited overlap with each other (15 and 23%). In contrast, GBF1 colocalizes with the cis-marker p115
(86%), whereas BIGs overlap extensively with TGN38
(83%). Consistent with these distributions, GBF1, but not
BIG1, partially relocalized to peripheral sites after
incubation at 15°C. The new GBF1 structures represent
peripheral vesicular tubular clusters (VTCs) because 88% of
structures analyzed stained for both GBF1 and p115.
Furthermore, as expected of VTCs, they rapidly reclustered
to the Golgi complex in a microtubule-dependent manner upon
warm-up. These observations suggest that GBF1 and BIGs
activate distinct subclasses of ARFs in specific locations to regulate different types of reactions. In agreement with
this possibility, COPI overlapped to a greater extent with
GBF1 (64%) than BIG1 (31%), whereas clathrin showed
limited overlap with BIG1, and virtually none with GBF1.
This article has been cited by other articles:
|
|
 |
|
  D. A. K. Dehring, A. S. Adler, A. Hosseini, and L. Hicke A C-terminal Sequence in the Guanine Nucleotide Exchange Factor Sec7 Mediates Golgi Association and Interaction with the Rsp5 Ubiquitin Ligase J. Biol. Chem., December 5, 2008; 283(49): 34188 - 34196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Shen, V. Meza-Carmen, E. Puxeddu, G. Wang, J. Moss, and M. Vaughan Interaction of brefeldin A-inhibited guanine nucleotide-exchange protein (BIG) 1 and kinesin motor protein KIF21A PNAS, December 2, 2008; 105(48): 18788 - 18793. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chun, Z. Shapovalova, S. Y. Dejgaard, J. F. Presley, and P. Melancon Characterization of Class I and II ADP-Ribosylation Factors (Arfs) in Live Cells: GDP-bound Class II Arfs Associate with the ER-Golgi Intermediate Compartment Independently of GBF1 Mol. Biol. Cell, August 1, 2008; 19(8): 3488 - 3500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ishizaki, H.-W. Shin, H. Mitsuhashi, and K. Nakayama Redundant Roles of BIG2 and BIG1, Guanine-Nucleotide Exchange Factors for ADP-Ribosylation Factors in Membrane Traffic between the trans-Golgi Network and Endosomes Mol. Biol. Cell, June 1, 2008; 19(6): 2650 - 2660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Citterio, A. Vichi, G. Pacheco-Rodriguez, A. M. Aponte, J. Moss, and M. Vaughan Unfolded protein response and cell death after depletion of brefeldin A-inhibited guanine nucleotide-exchange protein GBF1 PNAS, February 26, 2008; 105(8): 2877 - 2882. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Manolea, A. Claude, J. Chun, J. Rosas, and P. Melancon Distinct Functions for Arf Guanine Nucleotide Exchange Factors at the Golgi Complex: GBF1 and BIGs Are Required for Assembly and Maintenance of the Golgi Stack and trans-Golgi Network, Respectively Mol. Biol. Cell, February 1, 2008; 19(2): 523 - 535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Szul, R. Grabski, S. Lyons, Y. Morohashi, S. Shestopal, M. Lowe, and E. Sztul Dissecting the role of the ARF guanine nucleotide exchange factor GBF1 in Golgi biogenesis and protein trafficking J. Cell Sci., November 15, 2007; 120(22): 3929 - 3940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Ramaen, A. Joubert, P. Simister, N. Belgareh-Touze, M. C. Olivares-Sanchez, J.-C. Zeeh, S. Chantalat, M.-P. Golinelli-Cohen, C. L. Jackson, V. Biou, et al. Interactions between Conserved Domains within Homodimers in the BIG1, BIG2, and GBF1 Arf Guanine Nucleotide Exchange Factors J. Biol. Chem., September 28, 2007; 282(39): 28834 - 28842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yilmaz Dejgaard, A. Murshid, K. M. Dee, and J. F. Presley Confocal Microscopy-based Linescan Methodologies for Intra-Golgi Localization of Proteins J. Histochem. Cytochem., July 1, 2007; 55(7): 709 - 719. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Monetta, I. Slavin, N. Romero, and C. Alvarez Rab1b Interacts with GBF1 and Modulates both ARF1 Dynamics and COPI Association Mol. Biol. Cell, July 1, 2007; 18(7): 2400 - 2410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Wessels, D. Duijsings, K. H. W. Lanke, W. J. G. Melchers, C. L. Jackson, and F. J. M. van Kuppeveld Molecular Determinants of the Interaction between Coxsackievirus Protein 3A and Guanine Nucleotide Exchange Factor GBF1 J. Virol., May 15, 2007; 81(10): 5238 - 5245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Shen, M.-S. Hong, J. Moss, and M. Vaughan BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein, is required for correct glycosylation and function of integrin beta1 PNAS, January 23, 2007; 104(4): 1230 - 1235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhao, A. Claude, J. Chun, D. J. Shields, J. F. Presley, and P. Melancon GBF1, a cis-Golgi and VTCs-localized ARF-GEF, is implicated in ER-to-Golgi protein traffic J. Cell Sci., September 15, 2006; 119(18): 3743 - 3753. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ishizaki, H.-W. Shin, S. M. M. Iguchi-Ariga, H. Ariga, and K. Nakayama AMY-1 (associate of Myc-1) localization to the trans-Golgi network through interacting with BIG2, a guanine-nucleotide exchange factor for ADP-ribosylation factors. Genes Cells, August 1, 2006; 11(8): 949 - 959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Zeeh, M. Zeghouf, C. Grauffel, B. Guibert, E. Martin, A. Dejaegere, and J. Cherfils Dual Specificity of the Interfacial Inhibitor Brefeldin A for Arf Proteins and Sec7 Domains J. Biol. Chem., April 28, 2006; 281(17): 11805 - 11814. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Gillingham, J. R. C. Whyte, B. Panic, and S. Munro Mon2, a Relative of Large Arf Exchange Factors, Recruits Dop1 to the Golgi Apparatus J. Biol. Chem., January 27, 2006; 281(4): 2273 - 2280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-K. Park, L. M. Hartnell, and C. L. Jackson Mutations in a Highly Conserved Region of the Arf1p Activator GEA2 Block Anterograde Golgi Transport but Not COPI Recruitment to Membranes Mol. Biol. Cell, August 1, 2005; 16(8): 3786 - 3799. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Honda, O. S. Al-Awar, J. C. Hay, and J. G. Donaldson Targeting of Arf-1 to the early Golgi by membrin, an ER-Golgi SNARE J. Cell Biol., March 28, 2005; 168(7): 1039 - 1051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-K. Niu, A. C. Pfeifer, J. Lippincott-Schwartz, and C. L. Jackson Dynamics of GBF1, a Brefeldin A-Sensitive Arf1 Exchange Factor at the Golgi Mol. Biol. Cell, March 1, 2005; 16(3): 1213 - 1222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-F. Xu, X. Shen, H. Li, G. Pacheco-Rodriguez, J. Moss, and M. Vaughan Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70 PNAS, February 22, 2005; 102(8): 2784 - 2789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-W. Shin and K. Nakayama Guanine Nucleotide-Exchange Factors for Arf GTPases: Their Diverse Functions in Membrane Traffic J. Biochem., December 1, 2004; 136(6): 761 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Crespo, R. Iglesias-Bartolome, and J. L. Daniotti Ganglioside GD3 Traffics from the trans-Golgi Network to Plasma Membrane by a Rab11-independent and Brefeldin A-insensitive Exocytic Pathway J. Biol. Chem., November 12, 2004; 279(46): 47610 - 47618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Gillingham, A. H. Y. Tong, C. Boone, and S. Munro The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi J. Cell Biol., October 25, 2004; 167(2): 281 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Cox, R. J Mason-Gamer, C. L. Jackson, and N. Segev Phylogenetic Analysis of Sec7-Domain-containing Arf Nucleotide Exchangers Mol. Biol. Cell, April 1, 2004; 15(4): 1487 - 1505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chantalat, S.-K. Park, Z. Hua, K. Liu, R. Gobin, A. Peyroche, A. Rambourg, T. R. Graham, and C. L. Jackson The Arf activator Gea2p and the P-type ATPase Drs2p interact at the Golgi in Saccharomyces cerevisiae J. Cell Sci., February 15, 2004; 117(5): 711 - 722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chantalat, R. Courbeyrette, F. Senic-Matuglia, C. L. Jackson, B. Goud, and A. Peyroche A Novel Golgi Membrane Protein Is a Partner of the ARF Exchange Factors Gea1p and Gea2p Mol. Biol. Cell, June 1, 2003; 14(6): 2357 - 2371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Alvarez, R. Garcia-Mata, E. Brandon, and E. Sztul COPI Recruitment Is Modulated by a Rab1b-dependent Mechanism Mol. Biol. Cell, May 1, 2003; 14(5): 2116 - 2127. [Abstract] [Full Text] [PDF]
|
|
