![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 13, Issue 1, 12-24, January 2002
Actin-binding Site Is Important for Neurite
Outgrowth during Neuronal Differentiation
Department of Laboratory Medicine, Molecular Medicine, Lund
University, Malmö University Hospital, 205 02 Malmö, Sweden
We have previously shown that protein kinase C
(PKC) induces
neurite outgrowth via its regulatory domain and independently of its
kinase activity. This study aimed at identifying mechanisms regulating
PKC-mediated neurite induction. We show an increased association of
PKC to the cytoskeleton during neuronal differentiation. Furthermore, neurite induction by overexpression of full-length PKC
is suppressed if serum is removed from the cultures or if an
actin-binding site is deleted from the protein. A peptide corresponding to the PKC actin-binding site suppresses neurite outgrowth during neuronal differentiation and outgrowth elicited by PKC
overexpression. Neither serum removal, deletion of the actin-binding
site, nor introduction of the peptide affects neurite induction by the
isolated regulatory domain. Membrane targeting by myristoylation
renders full-length PKC independent of both serum and the
actin-binding site, and PKC colocalized with F-actin at the cortical
cytoskeleton during neurite outgrowth. These results demonstrate that
the actin-binding site is of importance for signals acting on PKC in
a pathway leading to neurite outgrowth. Localization of PKC to the
plasma membrane and/or the cortical cytoskeleton is conceivably
important for its effect on neurite outgrowth.
This article has been cited by other articles:
|
|
 |
|
  R. Gopalakrishna, U. Gundimeda, J. E. Schiffman, and T. H. McNeill A Direct Redox Regulation of Protein Kinase C Isoenzymes Mediates Oxidant-induced Neuritogenesis in PC12 Cells J. Biol. Chem., May 23, 2008; 283(21): 14430 - 14444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Li, J. M. Urban, M. L. Cayer, H. K. Plummer III, and C. A. Heckman Actin-based features negatively regulated by protein kinase C-{epsilon} Am J Physiol Cell Physiol, November 1, 2006; 291(5): C1002 - C1013. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. V. Jerdeva, F. A. Yarber, M. D. Trousdale, C. J. Rhodes, C. T. Okamoto, D. A. Dartt, and S. F. Hamm-Alvarez Dominant-negative PKC-{epsilon} impairs apical actin remodeling in parallel with inhibition of carbachol-stimulated secretion in rabbit lacrimal acini Am J Physiol Cell Physiol, October 1, 2005; 289(4): C1052 - C1068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ling, U. Troller, R. Zeidman, H. Stensman, A. Schultz, and C. Larsson Identification of Conserved Amino Acids N-terminal of the PKC{epsilon}C1b Domain Crucial for Protein Kinase C{epsilon}-mediated Induction of Neurite Outgrowth J. Biol. Chem., May 6, 2005; 280(18): 17910 - 17919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. T. Egelhoff, D. Croft, and P. A. Steimle Actin Activation of Myosin Heavy Chain Kinase A in Dictyostelium: A BIOCHEMICAL MECHANISM FOR THE SPATIAL REGULATION OF MYOSIN II FILAMENT DISASSEMBLY J. Biol. Chem., January 28, 2005; 280(4): 2879 - 2887. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sundberg, C. K. Thodeti, M. Kveiborg, C. Larsson, P. Parker, R. Albrechtsen, and U. M. Wewer Regulation of ADAM12 Cell-surface Expression by Protein Kinase C {epsilon} J. Biol. Chem., December 3, 2004; 279(49): 51601 - 51611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schultz, M. Ling, and C. Larsson Identification of an Amino Acid Residue in the Protein Kinase C C1b Domain Crucial for Its Localization to the Golgi Network J. Biol. Chem., July 23, 2004; 279(30): 31750 - 31760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. K. Thodeti, R. Albrechtsen, M. Grauslund, M. Asmar, C. Larsson, Y. Takada, A. M. Mercurio, J. R. Couchman, and U. M. Wewer ADAM12/Syndecan-4 Signaling Promotes beta 1 Integrin-dependent Cell Spreading through Protein Kinase Calpha and RhoA J. Biol. Chem., March 7, 2003; 278(11): 9576 - 9584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Liedtke, M. Hubbard, and X. Wang Stability of actin cytoskeleton and PKC-delta binding to actin regulate NKCC1 function in airway epithelial cells Am J Physiol Cell Physiol, February 1, 2003; 284(2): C487 - C496. [Abstract] [Full Text] [PDF]
|
|
