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Vol. 13, Issue 1, 225-237, January 2002
*Department of Cell Biology and Anatomy and
The absence of a direct route to the apical plasma membrane (PM)
for single transmembrane domain (TMD) proteins in polarized hepatic cells has been inferred but never directly demonstrated. The
genes encoding three pairs of apical PM proteins, whose extracellular domains are targeted exclusively to the apical milieu in Madin-Darby canine kidney cells, were packaged into recombinant adenovirus and
delivered to WIF-B cells in vitro and liver hepatocytes in vivo.
By immunofluorescence and pulse-chase metabolic labeling, we found that
the soluble constructs were overwhelmingly secreted into the
basolateral milieu, which in vivo is the blood and in vitro is the
culture medium. The full-length proteins were first delivered to the
basolateral surface but then concentrated in the apical PM. Our results
imply that hepatic cells lack trans-Golgi network (TGN)-based
machinery for directly sorting single transmembrane domain apical
proteins and raise interesting questions about current models of PM
protein sorting in polarized and nonpolarized cells.
Department of Neurosurgery, Johns Hopkins University
School of Medicine, Baltimore, Maryland 21205
Corresponding author. E-mail address:
alh{at}jhmi.edu.
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