![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 13, Issue 1, 276-284, January 2002
and
*Department of Cell and Molecular Biology,
Northwestern University Medical School, Chicago, IL 60611;
The recruitment of TATA binding protein (TBP)
to gene promoters is a critical rate-limiting step in
transcriptional
regulation for all three eukaryotic RNA polymerases.
However, little is known regarding the dynamics of TBP in
live mammalian cells. In this report, we examined the
distribution and dynamic behavior of green fluorescence
protein (GFP)-tagged TBP in live HeLa cells using fluorescence recovery after photobleaching (FRAP) analyses.
We observed that GFP-TBP associates with condensed
chromosomes throughout mitosis without any FRAP. These
results suggest that TBP stably associates with the
condensed chromosomes during mitosis. In addition, endogenous TBP and TBP-associated factors (TAFs), specific
for RNA polymerase II and III transcription, cofractionated
with mitotic chromatin, suggesting that TBP is retained as
a TBP-TAF complex on transcriptionally silent chromatin
throughout mitosis. In interphase cells, GFP-TBP
distributes throughout the nucleoplasm and shows a FRAP
that is 100-fold slower than the general transcription factor GFP-TFIIB. This difference supports the idea that
TBP and, most likely, TBP-TAF complexes, remain promoter-
bound for multiple rounds of transcription. Altogether, our
observations demonstrate that there are cell cycle specific
characteristics in the dynamic behavior of TBP. We propose
a novel model in which the association of TBP-TAF complexes
with chromatin during mitosis marks genes for rapid
transcriptional activation as cells emerge from mitosis.
Department of Biochemistry and Molecular
Biology, Michigan State University, East Lansing, MI 48824
This article has been cited by other articles:
|
|
 |
|
  Y.-X. Xu and J. L. Manley Pin1 modulates RNA polymerase II activity during the transcription cycle Genes & Dev., November 15, 2007; 21(22): 2950 - 2962. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-i. Komura, H. Ikehata, and T. Ono Chromatin fine structure of the c-MYC insulator element/DNase I-hypersensitive site I is not preserved during mitosis PNAS, October 2, 2007; 104(40): 15741 - 15746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. van den Boom, S. M. Kooistra, M. Boesjes, B. Geverts, A. B. Houtsmuller, K. Monzen, I. Komuro, J. Essers, L. J. Drenth-Diephuis, and B. J.L. Eggen UTF1 is a chromatin-associated protein involved in ES cell differentiation J. Cell Biol., September 7, 2007; 178(6): 913 - 924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Das, K. Hizume, K. Batta, B. R. P. Kumar, S. S. Gadad, S. Ganguly, S. Lorain, A. Verreault, P. P. Sadhale, K. Takeyasu, et al. Transcriptional Coactivator PC4, a Chromatin-Associated Protein, Induces Chromatin Condensation Mol. Cell. Biol., November 15, 2006; 26(22): 8303 - 8315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Marr II, Y. Isogai, K. J. Wright, and R. Tjian Coactivator cross-talk specifies transcriptional output Genes & Dev., June 1, 2006; 20(11): 1458 - 1469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. He and J. R. Davie Sp1 and Sp3 foci distribution throughout mitosis J. Cell Sci., March 15, 2006; 119(6): 1063 - 1070. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Matsumoto, S. Kim, and R. I. Morimoto Huntingtin and Mutant SOD1 Form Aggregate Structures with Distinct Molecular Properties in Human Cells J. Biol. Chem., February 17, 2006; 281(7): 4477 - 4485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Anobile, V. Arumugaswami, D. Downs, K. Czymmek, M. Parcells, and C. J. Schmidt Nuclear Localization and Dynamic Properties of the Marek's Disease Virus Oncogene Products Meq and Meq/vIL8 J. Virol., February 1, 2006; 80(3): 1160 - 1166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yan, L. Xu, G. Crawford, Z. Wang, and S. M. Burgess The Forkhead Transcription Factor FoxI1 Remains Bound to Condensed Mitotic Chromosomes and Stably Remodels Chromatin Structure Mol. Cell. Biol., January 1, 2006; 26(1): 155 - 168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-i. Komura and T. Ono Disappearance of Nucleosome Positioning in Mitotic Chromatin in Vivo J. Biol. Chem., April 15, 2005; 280(15): 14530 - 14535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Chen, M. Dundr, C. Wang, A. Leung, A. Lamond, T. Misteli, and S. Huang Condensed mitotic chromatin is accessible to transcription factors and chromatin structural proteins J. Cell Biol., January 3, 2005; 168(1): 41 - 54. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Zaidi, D. W. Young, J.-Y. Choi, J. Pratap, A. Javed, M. Montecino, J. L. Stein, J. B. Lian, A. J. van Wijnen, and G. S. Stein Intranuclear Trafficking: Organization and Assembly of Regulatory Machinery for Combinatorial Biological Control J. Biol. Chem., October 15, 2004; 279(42): 43363 - 43366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Kieffer-Kwon, I. Martianov, and I. Davidson Cell-specific Nucleolar Localization of TBP-related Factor 2 Mol. Biol. Cell, October 1, 2004; 15(10): 4356 - 4368. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Xie and J. N. Reeve Transcription by Methanothermobacter thermautotrophicus RNA Polymerase In Vitro Releases Archaeal Transcription Factor B but Not TATA-Box Binding Protein from the Template DNA J. Bacteriol., September 15, 2004; 186(18): 6306 - 6310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Phair, P. Scaffidi, C. Elbi, J. Vecerova, A. Dey, K. Ozato, D. T. Brown, G. Hager, M. Bustin, and T. Misteli Global Nature of Dynamic Protein-Chromatin Interactions In Vivo: Three-Dimensional Genome Scanning and Dynamic Interaction Networks of Chromatin Proteins Mol. Cell. Biol., July 15, 2004; 24(14): 6393 - 6402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fulco, A. Costanzo, P. Merlo, R. Mangiacasale, S. Strano, G. Blandino, C. Balsano, P. Lavia, and M. Levrero p73 Is Regulated by Phosphorylation at the G2/M Transition J. Biol. Chem., December 5, 2003; 278(49): 49196 - 49202. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Pallier, P. Scaffidi, S. Chopineau-Proust, A. Agresti, P. Nordmann, M. E. Bianchi, and V. Marechal Association of Chromatin Proteins High Mobility Group Box (HMGB) 1 and HMGB2 with Mitotic Chromosomes Mol. Biol. Cell, August 1, 2003; 14(8): 3414 - 3426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. T. Georgel, T. M. Fletcher, G. L. Hager, and J. C. Hansen Formation of higher-order secondary and tertiary chromatin structures by genomic mouse mammary tumor virus promoters Genes & Dev., July 1, 2003; 17(13): 1617 - 1629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. V. Prasanth, P. A. Sacco-Bubulya, S. G. Prasanth, and D. L. Spector Sequential Entry of Components of Gene Expression Machinery into Daughter Nuclei Mol. Biol. Cell, March 1, 2003; 14(3): 1043 - 1057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Handwerger, C. Murphy, and J. G. Gall Steady-state dynamics of Cajal body components in the Xenopus germinal vesicle J. Cell Biol., February 18, 2003; 160(4): 495 - 504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kimura, K. Sugaya, and P. R. Cook The transcription cycle of RNA polymerase II in living cells J. Cell Biol., December 9, 2002; 159(5): 777 - 782. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. I. Kumaran, B. Muralikrishna, and V. K. Parnaik Lamin A/C speckles mediate spatial organization of splicing factor compartments and RNA polymerase II transcription J. Cell Biol., December 9, 2002; 159(5): 783 - 793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Quadt, D. Mainz, R. Mans, A. Kremer, and D. Knebel-Morsdorf Baculovirus Infection Raises the Level of TATA-Binding Protein That Colocalizes with Viral DNA Replication Sites J. Virol., October 2, 2002; 76(21): 11123 - 11127. [Abstract] [Full Text] [PDF]
|
|
