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Vol. 13, Issue 1, 285-301, January 2002
Centre de Recherche de Biochimie Macromoléculaire, Centre
National de la Recherche Scientifique Unité Propre de Recherche
1086, 34293 Montpellier, France
Cadherin-mediated cell-cell adhesion is a dynamic process that is
regulated during embryonic development, cell migration, and
differentiation. Different cadherins are expressed in specific tissues
consistent with their roles in cell type recognition. In this study, we
examine the formation of N-cadherin-dependent cell-cell contacts in
fibroblasts and myoblasts. In contrast to E-cadherin, both endogenous
and ectopically expressed N-cadherin shuttles between an intracellular
and a plasma membrane pool. Initial formation of N-cadherin-dependent
cell-cell contacts results from the recruitment of the intracellular
pool of N-cadherin to the plasma membrane. N-cadherin also localizes to
the Golgi apparatus and both secretory and endocytotic vesicles. We
demonstrate that the intracellular pool of N-cadherin is tightly
associated with the microtubule (MT) network and that junction
formation requires MTs. In addition, localization of N-cadherin to the
cortex is dependent on an intact F-actin cytoskeleton. We show that
N-cadherin transport requires the MT network as well as the activity of
the MT-associated motor kinesin. In conclusion, we propose that
N-cadherin distribution is a regulated process promoted by cell-cell
contact formation, which controls the biogenesis and turnover of the
junctions through the MT network.
Online version of this article contains video material for
Figures 4, 5, 8, and 9. Online version available at
www.molbiolcell.org.
*
Corresponding author. E-mail address:
gauthier{at}crbm.cnrs-mop.fr.
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