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Vol. 13, Issue 10, 3646-3661, October 2002

Novel Proteins Linking the Actin Cytoskeleton to the Endocytic Machinery in Saccharomyces cerevisiae

H. Dewar,^* D. T. Warren,^* F. C. Gardiner,^* C. G. Gourlay,^* N. Satish,^* M. R. Richardson,^* P. D. Andrews, and K. R. Ayscough^*§

 ^*The Henry Wellcome Laboratory for Cell Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom; and  Wellcome Trust Biocentre, Division of Molecular Cell Biology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland

The importance of coupling the process of endocytosis to factors regulating actin dynamics has been clearly demonstrated in yeast, and many proteins involved in these mechanisms have been identified and characterized. Here we demonstrate the importance of two additional cortical components, Ysc84p and Lsb5p, which together are essential for the organization of the actin cytoskeleton and for fluid phase endocytosis. Both Ysc84p and Lsb5p were identified through two-hybrid screens with different domains of the adaptor protein Sla1p. Ysc84p colocalizes with cortical actin and requires the presence of an intact actin cytoskeleton for its cortical localization. Ycl034w/Lsb5p localizes to the cell cortex but does not colocalize with actin. The Lsb5 protein contains putative VHS and GAT domains as well as an NPF motif, which are all domains characteristic of proteins involved in membrane trafficking. Deletion of either gene alone does not confer any dramatic phenotype on cells. However, deletion of both genes is lethal at elevated temperatures. Furthermore, at all temperatures this double mutant has depolarized actin and an almost undetectable level of fluid phase endocytosis. Our data demonstrate that Ysc84p and Lsb5p are important components of complexes involved in overlapping pathways coupling endocytosis with the actin cytoskeleton in yeast.

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^§ Corresponding author. E-mail address: k.ayscough{at}bio.gla.ac.uk.

Both authors contributed equally to this work.

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Molecular Biology of the Cell
Vol. 13, 3646-3661, October 2002
Copyright © 2002 by The American Society for Cell Biology

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