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Vol. 13, Issue 11, 3859-3869, November 2002
-Tubulin Is an Essential Component of the Centriole
*Department of Genetics, §Department of Molecular
Microbiology,Washington University School of Medicine, St. Louis,
Missouri 63110; and Centrioles and basal bodies are cylinders composed of nine triplet
microtubule blades that play essential roles in the centrosome and in
flagellar assembly. Chlamydomonas cells with the
bld2-1 mutation fail to assemble doublet and triplet
microtubules and have defects in cleavage furrow placement and meiosis.
Using positional cloning, we have walked 720 kb and identified a
13.2-kb fragment that contains Molecular, Cellular, and
Developmental Biology, University of Colorado, Boulder, Colorado 80309
-tubulin and rescues the Bld2
defects. The bld2-1 allele has a premature stop codon
and intragenic revertants replace the stop codon with glutamine,
glutamate, or lysine. Polyclonal antibodies to -tubulin show
peripheral labeling of full-length basal bodies and centrioles. Thus,
-tubulin is encoded by the BLD2 allele and
-tubulin plays a role in basal body/centriole morphogenesis.
Corresponding author. E-mail address:
dutcher{at}genetics.wustl.edu.
The order of the authors is alphabetical because
the experimental contributions are equally distributed.
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