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Vol. 13, Issue 11, 3915-3929, November 2002
Department of Cell Biology, University of Texas Southwestern
Medical School, Dallas, Texas 75235-9039
When fibroblasts are placed within a three-dimensional collagen
matrix, cell locomotion results in translocation of the flexible collagen fibrils of the matrix, a remodeling process that has been
implicated in matrix morphogenesis during development and wound repair.
In the current experiments, we studied formation and maturation of
cell-matrix interactions under conditions in which we could
distinguish local from global matrix remodeling. Local remodeling was
measured by the movement of collagen-embedded beads towards the cells.
Global remodeling was measured by matrix contraction. Our observations
show that no direct relationship occurs between protrusion and
retraction of cell extensions and collagen matrix remodeling. As
fibroblasts globally remodel the collagen matrix, however, their
overall morphology changes from dendritic to stellate/bipolar, and
cell-matrix interactions mature from punctate to focal adhesion
organization. The less well organized sites of cell-matrix interaction
are sufficient for translocating collagen fibrils, and focal adhesions
only form after a high degree of global remodeling occurs in the
presence of growth factors. Rho kinase activity is required for
maturation of fibroblast morphology and formation of focal adhesions
but not for translocation of collagen fibrils.
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