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Vol. 13, Issue 11, 4100-4109, November 2002
and§
The notion of a "plurifunctional" nucleolus is now well
established. However, molecular mechanisms underlying the biological processes occurring within this nuclear domain remain only partially understood. As a first step in elucidating these mechanisms we have
carried out a proteomic analysis to draw up a list of proteins present
within nucleoli of HeLa cells. This analysis allowed the identification
of 213 different nucleolar proteins. This catalog complements that of
the 271 proteins obtained recently by others, giving a total of
~350 different nucleolar proteins. Functional classification of these
proteins allowed outlining several biological processes taking place
within nucleoli. Bioinformatic analyses permitted the assignment of
hypothetical functions for 43 proteins for which no functional
information is available. Notably, a role in ribosome biogenesis was
proposed for 31 proteins. More generally, this functional
classification reinforces the plurifunctional nature of nucleoli and
provides convincing evidence that nucleoli may play a central role in
the control of gene expression. Finally, this analysis supports the
recent demonstration of a coupling of transcription and translation in
higher eukaryotes.
Central Clinical Chemistry Laboratory, Geneva
University Hospital, 1211 Geneva 14, Switzerland; and
Institut National de la Santé et de la Recherche
Médicale U369, Faculté de Médecine Lyon-R.T.H.
Laennec, 69372 Lyon, France
Online version of this article contains supplementary material.
Online version is available at www.molbiolcell.org.
*
These authors contribute equally to this work.
§
Corresponding author. E-mail address:
diaz{at}laennec.univ-lyon1.fr.
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