![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 13, Issue 12, 4167-4178, December 2002
Cancer Research UK London Research Institute, Lincoln's Inn Fields
Laboratories, Transcription Laboratory, London WC2A 3PX, United Kingdom
Signal-induced activation of the transcription factor serum
response factor (SRF) requires alterations in actin dynamics. SRF
activity can be inhibited by ectopic expression of 
-actin, either
because actin itself participates in SRF regulation or as a consequence
of cytoskeletal perturbations. To distinguish between these
possibilities, we studied actin mutants. Three mutant actins, G13R,
R62D, and a C-terminal VP16 fusion protein, were shown not to
polymerize in vivo, as judged by two-hybrid, immunofluorescence, and
cell fractionation studies. These actins effectively inhibited SRF
activation, as did wild-type actin, which increased the G-actin level
without altering the F:G-actin ratio. Physical interaction between SRF
and actin was not detectable by mammalian or yeast two-hybrid assays,
suggesting that SRF regulation involves an unidentified cofactor. SRF
activity was not blocked upon inhibition of CRM1-mediated nuclear
export by leptomycin B. Two actin mutants were identified, V159N and
S14C, whose expression favored F-actin formation and which strongly
activated SRF in the absence of external signals. These mutants seemed
unable to inhibit SRF activity, because their expression did not reduce
the absolute level of G-actin as assessed by DNase I binding. Taken
together, these results provide strong evidence that G-actin, or a
subpopulation of it, plays a direct role in signal transduction to SRF.
Cancer Research UK London Research Institute
comprises the Lincoln's Inn Fields and Clare Hall Laboratories of the
former Imperial Cancer Research Fund after the merger of the Imperial Cancer Research Fund with the Cancer Research Campaign in February 2002.
Corresponding author. E-mail address:
richard.treisman{at}cancer.org.uk.
This article has been cited by other articles:
|
|
 |
|
  T. Q. P. Noguchi, Y. Gomibuchi, K. Murakami, H. Ueno, K. Hirose, T. Wakabayashi, and T. Q. P. Uyeda Dominant Negative Mutant Actins Identified in Flightless Drosophila Can Be Classified into Three Classes J. Biol. Chem., February 12, 2010; 285(7): 4337 - 4347. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Han, G. Liu, J. Profirovic, J. Niu, and T. Voyno-Yasenetskaya Zyxin is involved in thrombin signaling via interaction with PAR-1 receptor FASEB J, December 1, 2009; 23(12): 4193 - 4206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Kumar and A. B. Lassar The Transcriptional Activity of Sox9 in Chondrocytes Is Regulated by RhoA Signaling and Actin Polymerization Mol. Cell. Biol., August 1, 2009; 29(15): 4262 - 4273. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. A. Hofmann, A. Arduini, S. M. Nicol, C. J. Camacho, J. L. Lessard, F. V. Fuller-Pace, and P. de Lanerolle SUMOylation of nuclear actin J. Cell Biol., July 27, 2009; 186(2): 193 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. P. Makarenkova, K. N. Gonzalez, W. B. Kiosses, and R. Meech Barx2 Controls Myoblast Fusion and Promotes MyoD-mediated Activation of the Smooth Muscle{alpha}-Actin Gene J. Biol. Chem., May 29, 2009; 284(22): 14866 - 14874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Stern, E. Debre, C. Stritt, J. Berger, G. Posern, and B. Knoll A Nuclear Actin Function Regulates Neuronal Motility by Serum Response Factor-Dependent Gene Transcription J. Neurosci., April 8, 2009; 29(14): 4512 - 4518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Vaillant, S. J. Copeland, C. Davis, S. F. Thurston, N. Abdennur, and J. W. Copeland Interaction of the N- and C-terminal Autoregulatory Domains of FRL2 Does Not Inhibit FRL2 Activity J. Biol. Chem., November 28, 2008; 283(48): 33750 - 33762. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Descot, M. Rex-Haffner, G. Courtois, D. Bluteau, A. Menssen, T. Mercher, O. A. Bernard, R. Treisman, and G. Posern OTT-MAL Is a Deregulated Activator of Serum Response Factor-Dependent Gene Expression Mol. Cell. Biol., October 15, 2008; 28(20): 6171 - 6181. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Higashida, S. Suetsugu, T. Tsuji, J. Monypenny, S. Narumiya, and N. Watanabe G-actin regulates rapid induction of actin nucleation by mDia1 to restore cellular actin polymers J. Cell Sci., October 15, 2008; 121(20): 3403 - 3412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Verdoni, N. Aoyama, A. Ikeda, and S. Ikeda Effect of destrin mutations on the gene expression profile in vivo Physiol Genomics, June 1, 2008; 34(1): 9 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Busche, A. Descot, S. Julien, H. Genth, and G. Posern Epithelial cell-cell contacts regulate SRF-mediated transcription via Rac-actin-MAL signalling J. Cell Sci., April 1, 2008; 121(7): 1025 - 1035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Pederson As functional nuclear actin comes into view, is it globular, filamentous, or both? J. Cell Biol., March 24, 2008; 180(6): 1061 - 1064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xie, E-J. Tan, S. Wee, E. Manser, L. Lim, and C.-G. Koh Functional interactions between phosphatase POPX2 and mDia modulate RhoA pathways J. Cell Sci., February 15, 2008; 121(4): 514 - 521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ye, J. Zhao, U. Hoffmann-Rohrer, and I. Grummt Nuclear myosin I acts in concert with polymeric actin to drive RNA polymerase I transcription Genes & Dev., February 1, 2008; 22(3): 322 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Guettler, M. K. Vartiainen, F. Miralles, B. Larijani, and R. Treisman RPEL Motifs Link the Serum Response Factor Cofactor MAL but Not Myocardin to Rho Signaling via Actin Binding Mol. Cell. Biol., January 15, 2008; 28(2): 732 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Su, D. Kondrikov, and E. R. Block beta-Actin: A Regulator of NOS-3 Sci. Signal., September 18, 2007; 2007(404): pe52 - pe52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Vartiainen, S. Guettler, B. Larijani, and R. Treisman Nuclear Actin Regulates Dynamic Subcellular Localization and Activity of the SRF Cofactor MAL Science, June 22, 2007; 316(5832): 1749 - 1752. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Muehlich, I. Cicha, C. D. Garlichs, B. Krueger, G. Posern, and M. Goppelt-Struebe Actin-dependent regulation of connective tissue growth factor Am J Physiol Cell Physiol, May 1, 2007; 292(5): C1732 - C1738. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Staus, A. L. Blaker, J. M. Taylor, and C. P. Mack Diaphanous 1 and 2 Regulate Smooth Muscle Cell Differentiation by Activating the Myocardin-Related Transcription Factors Arterioscler Thromb Vasc Biol, March 1, 2007; 27(3): 478 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Fazal, M. Minhajuddin, K. M. Bijli, J. L. McGrath, and A. Rahman Evidence for Actin Cytoskeleton-dependent and -independent Pathways for RelA/p65 Nuclear Translocation in Endothelial Cells J. Biol. Chem., February 9, 2007; 282(6): 3940 - 3950. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Clement, M. Stouffs, E. Bettiol, S. Kampf, K.-H. Krause, C. Chaponnier, and M. Jaconi Expression and function of {alpha}-smooth muscle actin during embryonic-stem-cell-derived cardiomyocyte differentiation J. Cell Sci., January 15, 2007; 120(2): 229 - 238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Goulart da Silva, G. Giannocco, M. F. Santos, and M. T. Nunes Thyroid Hormone Induction of Actin Polymerization in Somatotrophs of Hypothyroid Rats: Potential Repercussions in Growth Hormone Synthesis and Secretion Endocrinology, December 1, 2006; 147(12): 5777 - 5785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schonichen, M. Alexander, J. E. Gasteier, F. E. Cuesta, O. T. Fackler, and M. Geyer Biochemical Characterization of the Diaphanous Autoregulatory Interaction in the Formin Homology Protein FHOD1 J. Biol. Chem., February 24, 2006; 281(8): 5084 - 5093. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. McDonald, G. Carrero, C. Andrin, G. de Vries, and M. J. Hendzel Nucleoplasmic {beta}-actin exists in a dynamic equilibrium between low-mobility polymeric species and rapidly diffusing populations. J. Cell Biol., February 13, 2006; 172(4): 541 - 552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Favot, S. M. Hall, S. G. Haworth, and P. R. Kemp Cytoplasmic YY1 Is Associated with Increased Smooth Muscle-Specific Gene Expression: Implications for Neonatal Pulmonary Hypertension Am. J. Pathol., December 1, 2005; 167(6): 1497 - 1509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. del Rio, C. J. DeCoste, and L.W. Enquist Actin Is a Component of the Compensation Mechanism in Pseudorabies Virus Virions Lacking the Major Tegument Protein VP22 J. Virol., July 1, 2005; 79(13): 8614 - 8619. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kuwahara, T. Barrientos, G. C. T. Pipes, S. Li, and E. N. Olson Muscle-Specific Signaling Mechanism That Links Actin Dynamics to Serum Response Factor Mol. Cell. Biol., April 15, 2005; 25(8): 3173 - 3181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Woods, G. Wang, and F. Beier RhoA/ROCK Signaling Regulates Sox9 Expression and Actin Organization during Chondrogenesis J. Biol. Chem., March 25, 2005; 280(12): 11626 - 11634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hu, S. Wu, and N. Hernandez A role for {beta}-actin in RNA polymerase III transcription Genes & Dev., December 15, 2004; 18(24): 3010 - 3015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Copeland, S. J. Copeland, and R. Treisman Homo-oligomerization Is Essential for F-actin Assembly by the Formin Family FH2 Domain J. Biol. Chem., November 26, 2004; 279(48): 50250 - 50256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Haller, I. Rambaldi, E. Daniels, and M. Featherstone Subcellular Localization of Multiple PREP2 Isoforms Is Regulated by Actin, Tubulin, and Nuclear Export J. Biol. Chem., November 19, 2004; 279(47): 49384 - 49394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ruegg, F. Holsboer, C. Turck, and T. Rein Cofilin 1 Is Revealed as an Inhibitor of Glucocorticoid Receptor by Analysis of Hormone-Resistant Cells Mol. Cell. Biol., November 1, 2004; 24(21): 9371 - 9382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhuang, G. T. Nguyen, Y. Chen, T. Gudi, M. Eigenthaler, T. Jarchau, U. Walter, G. R. Boss, and R. B. Pilz Vasodilator-stimulated Phosphoprotein Activation of Serum-response Element-dependent Transcription Occurs Downstream of RhoA and Is Inhibited by cGMP-dependent Protein Kinase Phosphorylation J. Biol. Chem., March 12, 2004; 279(11): 10397 - 10407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wang, H. Chen, A. Seth, and C. A. McCulloch Mechanical force regulation of myofibroblast differentiation in cardiac fibroblasts Am J Physiol Heart Circ Physiol, November 1, 2003; 285(5): H1871 - H1881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Cen, A. Selvaraj, R. C. Burgess, J. K. Hitzler, Z. Ma, S. W. Morris, and R. Prywes Megakaryoblastic Leukemia 1, a Potent Transcriptional Coactivator for Serum Response Factor (SRF), Is Required for Serum Induction of SRF Target Genes Mol. Cell. Biol., September 15, 2003; 23(18): 6597 - 6608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Nevins and D. C. Thurmond Glucose regulates the cortical actin network through modulation of Cdc42 cycling to stimulate insulin secretion Am J Physiol Cell Physiol, September 1, 2003; 285(3): C698 - C710. [Abstract] [Full Text] [PDF]
|
|
