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Vol. 13, Issue 12, 4179-4194, December 2002

Regulation of IB Expression in Testis

Lucy M. Budde,^* Chun Wu,^* Christopher Tilman, Iris Douglas,^* and Sankar Ghosh^*

 ^*Section of Immunobiology and Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520; and  Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710

IB and IB are regulators of the nuclear factor-B (NF-B) transcription factor family. Both IBs bind to the same NF-B dimers and are widely expressed in different cells and tissues. To better understand how these two IB isoforms differ biologically, we have characterized the expression of IB in testis, a tissue in which IB is only minimally expressed. We have found that IB expression is localized within the haploid spermatid stages of spermatogenesis and follows the expression of nuclear NF-B. IB expression in haploid spermatids is likely regulated by Sox family proteins, members of which are also expressed within spermatids. We have shown that both SRY and Sox-5 can bind to multiple Sox binding sites found within the IB promoter and can enhance transcription of a reporter gene in transient transfection assays. We also demonstrate that IB mRNA is strongly expressed in developing male gonads. These results therefore suggest that IB may be a novel target for transcription factors of the HMG-box SRY/Sox family and imply a potential role for NF-B/IB in spermatogenesis.

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Corresponding author. E-mail address: sankar.ghosh{at}yale.edu.

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Molecular Biology of the Cell
Vol. 13, 4179-4194, December 2002
Copyright © 2002 by The American Society for Cell Biology

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