![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 13, Issue 12, 4279-4295, December 2002
*Departments of Surgery and Orthopedics, Regenerative
Bioengineering and Repair Laboratory, UCLA School of Medicine, Los
Angeles, California 90095; and Much of the work conducted on adult stem cells has focused
on mesenchymal stem cells (MSCs) found within the bone marrow stroma. Adipose tissue, like bone marrow, is derived from the embryonic mesenchyme and contains a stroma that is easily isolated. Preliminary studies have recently identified a putative stem cell population within
the adipose stromal compartment. This cell population, termed processed
lipoaspirate (PLA) cells, can be isolated from human lipoaspirates and,
like MSCs, differentiate toward the osteogenic, adipogenic, myogenic,
and chondrogenic lineages. To confirm whether adipose tissue contains
stem cells, the PLA population and multiple clonal isolates were
analyzed using several molecular and biochemical approaches. PLA cells
expressed multiple CD marker antigens similar to those observed on
MSCs. Mesodermal lineage induction of PLA cells and clones resulted in
the expression of multiple lineage-specific genes and proteins.
Furthermore, biochemical analysis also confirmed lineage-specific
activity. In addition to mesodermal capacity, PLA cells and clones
differentiated into putative neurogenic cells, exhibiting a
neuronal-like morphology and expressing several proteins consistent
with the neuronal phenotype. Finally, PLA cells exhibited unique
characteristics distinct from those seen in MSCs, including differences
in CD marker profile and gene expression.
Department of Medicine
and the Jonsson Comprehensive Cancer Center, Division of Hematology and
Oncology, UCLA School of Medicine, Los Angeles, California 90095
Online version of this article contains supplemental figures and
tables. Online version is available at www.molbiolcell.org.
Corresponding author. E-mail address:
zukpat{at}yahoo.com.
This article has been cited by other articles:
|
|
 |
|
  R. Atoui, D. Shum-Tim, and R. C.J. Chiu Myocardial regenerative therapy: immunologic basis for the potential "universal donor cells". Ann. Thorac. Surg., July 1, 2008; 86(1): 327 - 334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. K. Rebelatto, A. M. Aguiar, M. P. Moretao, A. C. Senegaglia, P. Hansen, F. Barchiki, J. Oliveira, J. Martins, C. Kuligovski, F. Mansur, et al. Dissimilar Differentiation of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, and Adipose Tissue Experimental Biology and Medicine, July 1, 2008; 233(7): 901 - 913. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Dimmeler and A. Leri Aging and Disease as Modifiers of Efficacy of Cell Therapy Circ. Res., June 6, 2008; 102(11): 1319 - 1330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Izadpanah, D. Kaushal, C. Kriedt, F. Tsien, B. Patel, J. Dufour, and B. A. Bunnell Long-term In vitro Expansion Alters the Biology of Adult Mesenchymal Stem Cells Cancer Res., June 1, 2008; 68(11): 4229 - 4238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Gong and L. E. Niklason Small-diameter human vessel wall engineered from bone marrow-derived mesenchymal stem cells (hMSCs) FASEB J, June 1, 2008; 22(6): 1635 - 1648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Rider, C. Dombrowski, A. A. Sawyer, G. H. B. Ng, D. Leong, D. W. Hutmacher, V. Nurcombe, and S. M. Cool Autocrine Fibroblast Growth Factor 2 Increases the Multipotentiality of Human Adipose-Derived Mesenchymal Stem Cells Stem Cells, June 1, 2008; 26(6): 1598 - 1608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. K. Hsu, J. C. Wang, N. Q. Liu, L. Krenek, P. A. Zuk, M. H. Hedrick, P. Benhaim, and J. R. Lieberman Stem Cells from Human Fat as Cellular Delivery Vehicles in an Athymic Rat Posterolateral Spine Fusion Model J. Bone Joint Surg. Am., May 1, 2008; 90(5): 1043 - 1052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K.E. Schwab, P. Hutchinson, and C.E. Gargett Identification of surface markers for prospective isolation of human endometrial stromal colony-forming cells Hum. Reprod., April 1, 2008; 23(4): 934 - 943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Sheyn, G. Pelled, Y. Zilberman, F. Talasazan, J. M. Frank, D. Gazit, and Z. Gazit Nonvirally Engineered Porcine Adipose Tissue-Derived Stem Cells: Use in Posterior Spinal Fusion Stem Cells, April 1, 2008; 26(4): 1056 - 1064. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Skupin, H. Kettenmann, U. Winkler, M. Wartenberg, H. Sauer, S. C. Tovey, C. W. Taylor, and M. Falcke How Does Intracellular Ca2+ Oscillate: By Chance or by the Clock? Biophys. J., March 15, 2008; 94(6): 2404 - 2411. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Gandia, A. Arminan, J. M. Garcia-Verdugo, E. Lledo, A. Ruiz, M D. Minana, J. Sanchez-Torrijos, R. Paya, V. Mirabet, F. Carbonell-Uberos, et al. Human Dental Pulp Stem Cells Improve Left Ventricular Function, Induce Angiogenesis, and Reduce Infarct Size in Rats with Acute Myocardial Infarction Stem Cells, March 1, 2008; 26(3): 638 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Dimmeler, J. Burchfield, and A. M. Zeiher Cell-Based Therapy of Myocardial Infarction Arterioscler. Thromb. Vasc. Biol., February 1, 2008; 28(2): 208 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Tomiyama, N. Murase, D. B. Stolz, H. Toyokawa, D. R. O'Donnell, D. M. Smith, J. R. Dudas, J. P. Rubin, and K. G. Marra Characterization of Transplanted Green Fluorescent Protein+ Bone Marrow Cells into Adipose Tissue Stem Cells, February 1, 2008; 26(2): 330 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. O. Traktuev, S. Merfeld-Clauss, J. Li, M. Kolonin, W. Arap, R. Pasqualini, B. H. Johnstone, and K. L. March A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks Circ. Res., January 4, 2008; 102(1): 77 - 85. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Madonna, J. T. Willerson, and Y.-J. Geng Myocardin A Enhances Telomerase Activities in Adipose Tissue Mesenchymal Cells and Embryonic Stem Cells Undergoing Cardiovascular Myogenic Differentiation Stem Cells, January 1, 2008; 26(1): 202 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Secco, E. Zucconi, N. M. Vieira, L. L.Q. Fogaca, A. Cerqueira, M. D. F. Carvalho, T. Jazedje, O. K. Okamoto, A. R. Muotri, and M. Zatz Multipotent Stem Cells from Umbilical Cord: Cord Is Richer than Blood! Stem Cells, January 1, 2008; 26(1): 146 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Valina, K. Pinkernell, Y.-H. Song, X. Bai, S. Sadat, R. J. Campeau, T. H. Le Jemtel, and E. Alt Intracoronary administration of autologous adipose tissue-derived stem cells improves left ventricular function, perfusion, and remodelling after acute myocardial infarction Eur. Heart J., November 1, 2007; 28(21): 2667 - 2677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.E. Schwab and C.E. Gargett Co-expression of two perivascular cell markers isolates mesenchymal stem-like cells from human endometrium Hum. Reprod., November 1, 2007; 22(11): 2903 - 2911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Chamberlain, J. Fox, B. Ashton, and J. Middleton Concise Review: Mesenchymal Stem Cells: Their Phenotype, Differentiation Capacity, Immunological Features, and Potential for Homing Stem Cells, November 1, 2007; 25(11): 2739 - 2749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Miyazaki, M. Hardjo, T. Masaka, K. Tomiyama, N. Mahmut, R. J. Medina, A. Niida, H. Sonegawa, G. Du, R. Yong, et al. Isolation of a Bone Marrow-Derived Stem Cell Line with High Proliferation Potential and Its Application for Preventing Acute Fatal Liver Failure Stem Cells, November 1, 2007; 25(11): 2855 - 2863. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Kim, Y. Wu, and C. M. Smas Characterization of ScAP-23, a new cell line from murine subcutaneous adipose tissue, identifies genes for the molecular definition of preadipocytes Physiol Genomics, October 19, 2007; 31(2): 328 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-S. Tsai, S.-M. Hwang, K.-D. Chen, Y.-S. Lee, L.-W. Hsu, Y.-J. Chang, C.-N. Wang, H.-H. Peng, Y.-L. Chang, A.-S. Chao, et al. Functional Network Analysis of the Transcriptomes of Mesenchymal Stem Cells Derived from Amniotic Fluid, Amniotic Membrane, Cord Blood, and Bone Marrow Stem Cells, October 1, 2007; 25(10): 2511 - 2523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wagner, C. Roderburg, F. Wein, A. Diehlmann, M. Frankhauser, R. Schubert, V. Eckstein, and A. D. Ho Molecular and Secretory Profiles of Human Mesenchymal Stromal Cells and Their Abilities to Maintain Primitive Hematopoietic Progenitors Stem Cells, October 1, 2007; 25(10): 2638 - 2647. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Medina, R. T. Kilani, N. Carr, E. Brown, and A. Ghahary Transdifferentiation of Peripheral Blood Mononuclear Cells into Epithelial-Like Cells Am. J. Pathol., October 1, 2007; 171(4): 1140 - 1152. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jones, N. Horwood, A. Cope, and F. Dazzi The Antiproliferative Effect of Mesenchymal Stem Cells Is a Fundamental Property Shared by All Stromal Cells J. Immunol., September 1, 2007; 179(5): 2824 - 2831. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. G. Chen, W. J. Zhang, D. Bi, W. Liu, X. Wei, F. F. Chen, L. Zhu, L. Cui, and Y. Cao Clonal analysis of nestin vimentin+ multipotent fibroblasts isolated from human dermis J. Cell Sci., August 15, 2007; 120(16): 2875 - 2883. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kucerova, V. Altanerova, M. Matuskova, S. Tyciakova, and C. Altaner Adipose Tissue-Derived Human Mesenchymal Stem Cells Mediated Prodrug Cancer Gene Therapy Cancer Res., July 1, 2007; 67(13): 6304 - 6313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sudo, M. Kanno, K. Miharada, S. Ogawa, T. Hiroyama, K. Saijo, and Y. Nakamura Mesenchymal Progenitors Able to Differentiate into Osteogenic, Chondrogenic, and/or Adipogenic Cells In Vitro Are Present in Most Primary Fibroblast-Like Cell Populations Stem Cells, July 1, 2007; 25(7): 1610 - 1617. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. T. Hennrick, A. G. Keeton, S. Nanua, T. G. Kijek, A. M. Goldsmith, U. S. Sajjan, J. K. Bentley, V. N. Lama, B. B. Moore, R. E. Schumacher, et al. Lung Cells from Neonates Show a Mesenchymal Stem Cell Phenotype Am. J. Respir. Crit. Care Med., June 1, 2007; 175(11): 1158 - 1164. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nishimura, I. Manabe, M. Nagasaki, Y. Hosoya, H. Yamashita, H. Fujita, M. Ohsugi, K. Tobe, T. Kadowaki, R. Nagai, et al. Adipogenesis in Obesity Requires Close Interplay Between Differentiating Adipocytes, Stromal Cells, and Blood Vessels Diabetes, June 1, 2007; 56(6): 1517 - 1526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Gimble, A. J. Katz, and B. A. Bunnell Adipose-Derived Stem Cells for Regenerative Medicine Circ. Res., May 11, 2007; 100(9): 1249 - 1260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Yamada, S.-i. Yokoyama, X.-D. Wang, N. Fukuda, and N. Takakura Cardiac Stem Cells in Brown Adipose Tissue Express CD133 and Induce Bone Marrow Nonhematopoietic Cells to Differentiate into Cardiomyocytes Stem Cells, May 1, 2007; 25(5): 1326 - 1333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. De Becker, P. Van Hummelen, M. Bakkus, I. Vande Broek, J. De Wever, M. De Waele, and I. Van Riet Migration of culture-expanded human mesenchymal stem cells through bone marrow endothelium is regulated by matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-3 Haematologica, April 1, 2007; 92(4): 440 - 449. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schaffler and C. Buchler Concise Review: Adipose Tissue-Derived Stromal Cells--Basic and Clinical Implications for Novel Cell-Based Therapies Stem Cells, April 1, 2007; 25(4): 818 - 827. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. E. Rice, E. W. Hsu, H. Sheng, D. A. Evenson, A. J. Freemerman, K. M. Safford, J. M. Provenzale, D. S. Warner, and G. A. Johnson Superparamagnetic Iron Oxide Labeling and Transplantation of Adipose-Derived Stem Cells in Middle Cerebral Artery Occlusion-Injured Mice Am. J. Roentgenol., April 1, 2007; 188(4): 1101 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-H. Cho, Y. Jun Koh, J. Han, H.-K. Sung, H. Jong Lee, T. Morisada, R. A. Schwendener, R. A. Brekken, G. Kang, Y. Oike, et al. Angiogenic Role of LYVE-1-Positive Macrophages in Adipose Tissue Circ. Res., March 2, 2007; 100(4): e47 - e57. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Liu, M. Martina, D. W. Hutmacher, J. H. P. Hui, E. H. Lee, and B. Lim Identification of Common Pathways Mediating Differentiation of Bone Marrow- and Adipose Tissue-Derived Human Mesenchymal Stem Cells into Three Mesenchymal Lineages Stem Cells, March 1, 2007; 25(3): 750 - 760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Koga, T. Muneta, Y.-J. Ju, T. Nagase, A. Nimura, T. Mochizuki, S. Ichinose, K. von der Mark, and I. Sekiya Synovial Stem Cells Are Regionally Specified According to Local Microenvironments After Implantation for Cartilage Regeneration Stem Cells, March 1, 2007; 25(3): 689 - 696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-E. Zaragosi, N. Billon, G. Ailhaud, and C. Dani Nucleofection Is a Valuable Transfection Method for Transient and Stable Transgene Expression in Adipose Tissue-Derived Stem Cells Stem Cells, March 1, 2007; 25(3): 790 - 797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Halaschek-Wiener and A. Brooks-Wilson Progeria of Stem Cells: Stem Cell Exhaustion in Hutchinson-Gilford Progeria Syndrome J. Gerontol. A Biol. Sci. Med. Sci., January 1, 2007; 62(1): 3 - 8. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.E. Gargett Uterine stem cells: What is the evidence? Hum. Reprod. Update, January 1, 2007; 13(1): 87 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. He, C. Wan, and G. Li Concise Review: Multipotent Mesenchymal Stromal Cells in Blood Stem Cells, January 1, 2007; 25(1): 69 - 77. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Trujillo and P. E. Scherer Adipose Tissue-Derived Factors: Impact on Health and Disease Endocr. Rev., December 1, 2006; 27(7): 762 - 778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Rink, S. Roy, S. Khanna, T. Rink, D. Bagchi, and C. K. Sen Transcriptome of the subcutaneous adipose tissue in response to oral supplementation of type 2 Leprdb obese diabetic mice with niacin-bound chromium Physiol Genomics, November 21, 2006; 27(3): 370 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. H. Wu, Y. L. Liu, B. Zhou, and Z. C. Han Cellular therapy and myocardial tissue engineering: the role of adult stem and progenitor cells Eur. J. Cardiothorac. Surg., November 1, 2006; 30(5): 770 - 781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.J. Mao, W.V. Giannobile, J.A. Helms, S.J. Hollister, P.H. Krebsbach, M.T. Longaker, and S. Shi Craniofacial tissue engineering by stem cells. J. Dent. Res., November 1, 2006; 85(11): 966 - 979. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-E. Zaragosi, G. Ailhaud, and C. Dani Autocrine Fibroblast Growth Factor 2 Signaling Is Critical for Self-Renewal of Human Multipotent Adipose-Derived Stem Cells Stem Cells, November 1, 2006; 24(11): 2412 - 2419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Yanez, M. L. Lamana, J. Garcia-Castro, I. Colmenero, M. Ramirez, and J. A. Bueren Adipose Tissue-Derived Mesenchymal Stem Cells Have In Vivo Immunosuppressive Properties Applicable for the Control of the Graft-Versus-Host Disease Stem Cells, November 1, 2006; 24(11): 2582 - 2591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Tjabringa, P. S. Vezeridis, B. Zandieh-Doulabi, M. N. Helder, P. I.J.M. Wuisman, and J. Klein-Nulend Polyamines Modulate Nitric Oxide Production and Cox-2 Gene Expression in Response to Mechanical Loading in Human Adipose Tissue-Derived Mesenchymal Stem Cells Stem Cells, October 1, 2006; 24(10): 2262 - 2269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Wan, Y.-Y. Shi, R. P. Nacamuli, N. Quarto, K. M. Lyons, and M. T. Longaker Osteogenic differentiation of mouse adipose-derived adult stromal cells requires retinoic acid and bone morphogenetic protein receptor type IB signaling PNAS, August 15, 2006; 103(33): 12335 - 12340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. V. Rodriguez, Z. Alfonso, R. Zhang, J. Leung, B. Wu, and L. J. Ignarro Clonogenic multipotent stem cells in human adipose tissue differentiate into functional smooth muscle cells PNAS, August 8, 2006; 103(32): 12167 - 12172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang, F. Li, and C. Niyibizi Progenitors Systemically Transplanted into Neonatal Mice Localize to Areas of Active Bone Formation In Vivo: Implications of Cell Therapy for Skeletal Diseases Stem Cells, August 1, 2006; 24(8): 1869 - 1878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chan, K. O'Donoghue, M. Gavina, Y. Torrente, N. Kennea, H. Mehmet, H. Stewart, D. J. Watt, J. E. Morgan, and N. M. Fisk Galectin-1 Induces Skeletal Muscle Differentiation in Human Fetal Mesenchymal Stem Cells and Increases Muscle Regeneration Stem Cells, August 1, 2006; 24(8): 1879 - 1891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-C. Chien, B. L. Yen, F.-K. Lee, T.-H. Lai, Y.-C. Chen, S.-H. Chan, and H.-I Huang In Vitro Differentiation of Human Placenta-Derived Multipotent Cells into Hepatocyte-Like Cells Stem Cells, July 1, 2006; 24(7): 1759 - 1768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sarig, Z. Baruchi, O. Fuchs, U. Nudel, and D. Yaffe Regeneration and Transdifferentiation Potential of Muscle-Derived Stem Cells Propagated as Myospheres Stem Cells, July 1, 2006; 24(7): 1769 - 1778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Aslan, Y. Zilberman, L. Kandel, M. Liebergall, R. J. Oskouian, D. Gazit, and Z. Gazit Osteogenic Differentiation of Noncultured Immunoisolated Bone Marrow-Derived CD105+ Cells Stem Cells, July 1, 2006; 24(7): 1728 - 1737. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kern, H. Eichler, J. Stoeve, H. Kluter, and K. Bieback Comparative Analysis of Mesenchymal Stem Cells from Bone Marrow, Umbilical Cord Blood, or Adipose Tissue Stem Cells, May 1, 2006; 24(5): 1294 - 1301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. McIntosh, S. Zvonic, S. Garrett, J. B. Mitchell, Z. E. Floyd, L. Hammill, A. Kloster, Y. Di Halvorsen, J. P. Ting, R. W. Storms, et al. The Immunogenicity of Human Adipose-Derived Cells: Temporal Changes In Vitro Stem Cells, May 1, 2006; 24(5): 1246 - 1253. [Abstract] |
