![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 13, Issue 12, 4355-4370, December 2002
and 
Are Essential for
Spindle Formation and Nuclear Envelope Assembly in Living
Caenorhabditis elegans Embryos
European Molecular Biology Laboratory, 69117 Heidelberg, Germany
The small GTPase Ran has been found to play pivotal roles in
several aspects of cell function. We have investigated the role of the
Ran GTPase cycle in spindle formation and nuclear envelope assembly in
dividing Caenorhabditis elegans embryos in real time. We
found that Ran and its cofactors RanBP2, RanGAP, and RCC1 are all
essential for reformation of the nuclear envelope after cell division.
Reducing the expression of any of these components of the Ran GTPase
cycle by RNAi leads to strong extranuclear clustering of integral
nuclear envelope proteins and nucleoporins. Ran, RanBP2, and RanGAP are
also required for building a mitotic spindle, whereas astral
microtubules are normal in the absence of these proteins. RCC1(RNAi)
embryos have similar abnormalities in the initial phase of spindle
formation but eventually recover to form a bipolar spindle. Irregular
chromatin structures and chromatin bridges due to spindle failure were
frequently observed in embryos where the Ran cycle was perturbed. In
addition, connection between the centrosomes and the male pronucleus,
and thus centrosome positioning, depends upon the Ran cycle components.
Finally, we have demonstrated that both IMA-2 and IMB-1, the homologues
of vertebrate importin 
and 
, are essential for both spindle
assembly and nuclear formation in early embryos.
Online version of this
article contains video material for some figures. Online version
available at www.molbiolcell.org.
Corresponding author. E-mail address:
mattaj{at}embl-heidelberg.de.
This article has been cited by other articles:
|
|
 |
|
  P. Kalab and R. Heald The RanGTP gradient - a GPS for the mitotic spindle J. Cell Sci., May 15, 2008; 121(10): 1577 - 1586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tegha-Dunghu, B. Neumann, S. Reber, R. Krause, H. Erfle, T. Walter, M. Held, P. Rogers, K. Hupfeld, T. Ruppert, et al. EML3 is a nuclear microtubule-binding protein required for the correct alignment of chromosomes in metaphase J. Cell Sci., May 15, 2008; 121(10): 1718 - 1726. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Adam, K. Sengupta, and R. D. Goldman Regulation of Nuclear Lamin Polymerization by Importin {alpha} J. Biol. Chem., March 28, 2008; 283(13): 8462 - 8468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Galy, W. Antonin, A. Jaedicke, M. Sachse, R. Santarella, U. Haselmann, and I. Mattaj A role for gp210 in mitotic nuclear-envelope breakdown J. Cell Sci., February 1, 2008; 121(3): 317 - 328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Anderson and M. W. Hetzer Shaping the endoplasmic reticulum into the nuclear envelope J. Cell Sci., January 15, 2008; 121(2): 137 - 142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Tedeschi, M. Ciciarello, R. Mangiacasale, E. Roscioli, W. M. Rensen, and P. Lavia RANBP1 localizes a subset of mitotic regulatory factors on spindle microtubules and regulates chromosome segregation in human cells J. Cell Sci., November 1, 2007; 120(21): 3748 - 3761. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Kemp, M. H. Song, M. K. Addepalli, G. Hunter, and K. O'Connell Suppressors of zyg-1 Define Regulators of Centrosome Duplication and Nuclear Association in Caenorhabditis elegans Genetics, May 1, 2007; 176(1): 95 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ma, S. Cai, Q. Lv, Q. Jiang, Q. Zhang, Sodmergen, Z. Zhai, and C. Zhang Lamin B receptor plays a role in stimulating nuclear envelope production and targeting membrane vesicles to chromatin during nuclear envelope assembly through direct interaction with importin beta J. Cell Sci., February 1, 2007; 120(3): 520 - 530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Dumont, S. Petri, F. Pellegrin, M.-E. Terret, M. T. Bohnsack, P. Rassinier, V. Georget, P. Kalab, O. J. Gruss, and M.-H. Verlhac A centriole- and RanGTP-independent spindle assembly pathway in meiosis I of vertebrate oocytes J. Cell Biol., January 29, 2007; 176(3): 295 - 305. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Orjalo, A. Arnaoutov, Z. Shen, Y. Boyarchuk, S. G. Zeitlin, B. Fontoura, S. Briggs, M. Dasso, and D. J. Forbes The Nup107-160 Nucleoporin Complex Is Required for Correct Bipolar Spindle Assembly Mol. Biol. Cell, September 1, 2006; 17(9): 3806 - 3818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. O'Brien and C. Wiese TPX2 is required for postmitotic nuclear assembly in cell-free Xenopus laevis egg extracts J. Cell Biol., June 5, 2006; 173(5): 685 - 694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. V. Silverman-Gavrila and A. Wilde Ran Is Required before Metaphase for Spindle Assembly and Chromosome Alignment and after Metaphase for Chromosome Segregation and Spindle Midbody Organization Mol. Biol. Cell, April 1, 2006; 17(4): 2069 - 2080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-K. Cao, Z.-S. Zhong, D.-Y. Chen, G.-X. Zhang, H. Schatten, and Q.-Y. Sun Cell cycle-dependent localization and possible roles of the small GTPase Ran in mouse oocyte maturation, fertilization and early cleavage Reproduction, October 1, 2005; 130(4): 431 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. R. Forsthoefel, K. Cutler, M. D. Port, T. Yamamoto, and D. M. Vernon PIRLs: A Novel Class of Plant Intracellular Leucine-rich Repeat Proteins Plant Cell Physiol., June 1, 2005; 46(6): 913 - 922. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Poteryaev, J. M. Squirrell, J. M. Campbell, J. G. White, and A. Spang Involvement of the Actin Cytoskeleton and Homotypic Membrane Fusion in ER Dynamics in Caenorhabditis elegans Mol. Biol. Cell, May 1, 2005; 16(5): 2139 - 2153. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Bhatti and W. J. Sullivan Jr. Histone Acetylase GCN5 Enters the Nucleus via Importin-{alpha} in Protozoan Parasite Toxoplasma gondii J. Biol. Chem., February 18, 2005; 280(7): 5902 - 5908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ciciarello, R. Mangiacasale, C. Thibier, G. Guarguaglini, E. Marchetti, B. Di Fiore, and P. Lavia Importin {beta} is transported to spindle poles during mitosis and regulates Ran-dependent spindle assembly factors in mammalian cells J. Cell Sci., December 15, 2004; 117(26): 6511 - 6522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Quensel, B. Friedrich, T. Sommer, E. Hartmann, and M. Kohler In Vivo Analysis of Importin {alpha} Proteins Reveals Cellular Proliferation Inhibition and Substrate Specificity Mol. Cell. Biol., December 1, 2004; 24(23): 10246 - 10255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Davies, A. H. Osmani, C. P. C. De Souza, C. Bachewich, and S. A. Osmani Potential Link between the NIMA Mitotic Kinase and Nuclear Membrane Fission during Mitotic Exit in Aspergillus nidulans Eukaryot. Cell, December 1, 2004; 3(6): 1433 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. J. Gruss and I. Vernos The mechanism of spindle assembly: functions of Ran and its target TPX2 J. Cell Biol., September 27, 2004; 166(7): 949 - 955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Loiodice, A. Alves, G. Rabut, M. van Overbeek, J. Ellenberg, J.-B. Sibarita, and V. Doye The Entire Nup107-160 Complex, Including Three New Members, Is Targeted as One Entity to Kinetochores in Mitosis Mol. Biol. Cell, July 1, 2004; 15(7): 3333 - 3344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Du, S. Ferro-Novick, and P. Novick Dynamics and inheritance of the endoplasmic reticulum J. Cell Sci., June 15, 2004; 117(14): 2871 - 2878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Ems-McClung, Y. Zheng, and C. E. Walczak Importin {alpha}/{beta} and Ran-GTP Regulate XCTK2 Microtubule Binding through a Bipartite Nuclear Localization Signal Mol. Biol. Cell, January 1, 2004; 15(1): 46 - 57. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Mason, E. Mathe, R. J. Fleming, and D. S. Goldfarb The Drosophila melanogaster importin {alpha}3 Locus Encodes an Essential Gene Required for the Development of Both Larval and Adult Tissues Genetics, December 1, 2003; 165(4): 1943 - 1958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Galy, I. W. Mattaj, and P. Askjaer Caenorhabditis elegans Nucleoporins Nup93 and Nup205 Determine the Limit of Nuclear Pore Complex Size Exclusion In Vivo Mol. Biol. Cell, December 1, 2003; 14(12): 5104 - 5115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Segbert, R. Barkus, J. Powers, S. Strome, W. M. Saxton, and O. Bossinger KLP-18, a Klp2 Kinesin, Is Required for Assembly of Acentrosomal Meiotic Spindles in Caenorhabditis elegans Mol. Biol. Cell, November 1, 2003; 14(11): 4458 - 4469. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Harel, R. C. Chan, A. Lachish-Zalait, E. Zimmerman, M. Elbaum, and D. J. Forbes Importin {beta} Negatively Regulates Nuclear Membrane Fusion and Nuclear Pore Complex Assembly Mol. Biol. Cell, November 1, 2003; 14(11): 4387 - 4396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Salina, P. Enarson, J.B. Rattner, and B. Burke Nup358 integrates nuclear envelope breakdown with kinetochore assembly J. Cell Biol., September 15, 2003; 162(6): 991 - 1001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Le Masson, D. Y. Yu, K. Jensen, A. Chevalier, R. Courbeyrette, Y. Boulard, M. M. Smith, and C. Mann Yaf9, a Novel NuA4 Histone Acetyltransferase Subunit, Is Required for the Cellular Response to Spindle Stress in Yeast Mol. Cell. Biol., September 1, 2003; 23(17): 6086 - 6102. [Abstract] [Full Text] [PDF]
|
|
