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Originally published as MBC in Press, 10.1091/mbc.01-06-0291 on January 18, 2002
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Vol. 13, Issue 2, 393-401, February 2002

Post-Cytochrome c Protection from Apoptosis Conferred by a MAPK Pathway in Xenopus Egg Extracts

Jessica S. Tashker, Michael Olson, and Sally Kornbluth*

Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710

In response to many different apoptotic stimuli, cytochrome c is released from the intermembrane space of the mitochondria into the cytoplasm, where it serves as a cofactor in the activation of procaspase 9. Inhibition of this process can occur either by preventing cytochrome c release or by blocking caspase activation or activity. Experiments involving in vitro reconstitution of apoptosis in cell-free extracts of Xenopus laevis eggs have suggested that extracts arrested in interphase are susceptible to an endogenous apoptotic program leading to caspase activation, whereas extracts arrested in meiotic metaphase are not. We report here that Mos/MEK/MAPK pathways active in M phase-arrested eggs are responsible for rendering them refractory to apoptosis. Interestingly, M phase-arrested extracts are competent to release cytochrome c, yet still do not activate caspases. Concomitantly, we have also demonstrated that recombinant Mos, MEK, and ERK are sufficient to block cytochrome c-dependent caspase activation in purified Xenopus cytosol, which lacks both transcription and translation. These data indicate that the MAP kinase pathway can target and inhibit post-cytochrome c release apoptotic events in the absence of new mRNA/protein synthesis and that this biochemical pathway is responsible for the apoptotic inhibition observed in meiotic X. laevis egg extracts.

* Corresponding author. E-mail address: kornb001{at}mc.duke.edu.

Molecular Biology of the Cell
Vol. 13, 393-401, February 2002
Copyright © 2002 by The American Society for Cell Biology


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