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Vol. 13, Issue 2, 632-645, February 2002
*Department of Cell Biology and Physiology, University of
California, San Francisco, San Francisco, California 94143; and
The budding yeast YCS4 gene encodes a conserved
regulatory subunit of the condensin complex. We isolated an allele of
this gene in a screen for mutants defective in sister chromatid
separation or segregation. The phenotype of the ycs4-1
mutant is similar to topoisomerase II mutants and distinct from the
esp1-1 mutant: the topological resolution of sister
chromatids is compromised in ycs4-1 despite normal
removal of cohesins from mitotic chromosomes. Consistent with a role in
sister separation, YCS4 function is required to localize
DNA topoisomerase I and II to chromosomes. Unlike its homologs in
Xenopus and fission yeast, Ycs4p is associated with
chromatin throughout the cell cycle; the only change in localization occurs during anaphase when the protein is enriched at the nucleolus. This relocalization may reveal the specific challenge that segregation of the transcriptionally hyperactive, repetitive array of rDNA genes
can present during mitosis. Indeed, segregation of the nucleolus is
abnormal in ycs4-1 at the nonpermissive temperature.
Interrepeat recombination in the rDNA array is specifically elevated in
ycs4-1 at the permissive temperature, suggesting that
the Ycs4p plays a role at the array aside from its segregation.
Furthermore, ycs4-1 is defective in silencing at the
mating type loci at the permissive temperature. Taken together, our
data suggest that there are mitotic as well as nonmitotic chromosomal
abnormalities associated with loss of condensin function in budding yeast.
Department of Molecular and Cell Biology, Harvard
University, Cambridge, Massachusetts 02138
Corresponding author. E-mail address:
amurray{at}mcb.harvard.edu.
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