![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 13, Issue 2, 670-682, February 2002
Departments of Microbiology and Urology and The Kaplan
Comprehensive Cancer Center, New York University School of Medicine,
New York, New York 10016
The androgen receptor (AR) is a ligand-regulated transcription
factor that stimulates cell growth and differentiation in
androgen-responsive tissues. The AR N terminus contains two activation
functions (AF-1a and AF-1b) that are necessary for maximal
transcriptional enhancement by the receptor; however, the mechanisms
and components regulating AR transcriptional activation are not fully
understood. We sought to identify novel factors that interact with the
AR N terminus from an androgen-stimulated human prostate cancer cell
library using a yeast two-hybrid approach designed to identify proteins that interact with transcriptional activation domains. A 157-amino acid
protein termed ART-27 was cloned and shown to interact predominantly with the AR153-336, containing AF-1a and a part of AF-1b, localize to the nucleus and increase the transcriptional activity of AR
when overexpressed in cultured mammalian cells. ART-27 also enhanced
the transcriptional activation by AR153-336 fused to the
LexA DNA-binding domain but not other AR N-terminal subdomains, suggesting that ART-27 exerts its effect via an interaction with a
defined region of the AR N terminus. ART-27 interacts with AR in
nuclear extracts from LNCaP cells in a ligand-independent manner. Interestingly, velocity gradient sedimentation of HeLa nuclear extracts
suggests that native ART-27 is part of a multiprotein complex. ART-27
is expressed in a variety of human tissues, including sites of androgen
action such as prostate and skeletal muscle, and is conserved
throughout evolution. Thus, ART-27 is a novel cofactor that interacts
with the AR N terminus and plays a role in facilitating
receptor-induced transcriptional activation.
This article has been cited by other articles:
|
|
 |
|
  J. C. Nwachukwu, P. Mita, R. Ruoff, S. Ha, Q. Wang, S. J. Huang, S. S. Taneja, M. Brown, W. L. Gerald, M. J. Garabedian, et al. Genome-Wide Impact of Androgen Receptor Trapped clone-27 Loss on Androgen-Regulated Transcription in Prostate Cancer Cells Cancer Res., April 1, 2009; 69(7): 3140 - 3147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Suh, E.-Y. Gong, J. B. Kim, I.-K. Lee, H.-S. Choi, and K. Lee Sterol Regulatory Element-Binding Protein-1c Represses the Transactivation of Androgen Receptor and Androgen-Dependent Growth of Prostatic Cells Mol. Cancer Res., February 1, 2008; 6(2): 314 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Suh, M. Shong, H.-S. Choi, and K. Lee CR6-Interacting Factor 1 Represses the Transactivation of Androgen Receptor by Direct Interaction Mol. Endocrinol., January 1, 2008; 22(1): 33 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. V. Heemers and D. J. Tindall Androgen Receptor (AR) Coregulators: A Diversity of Functions Converging on and Regulating the AR Transcriptional Complex Endocr. Rev., December 1, 2007; 28(7): 778 - 808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Nwachukwu, W. Li, I. Pineda-Torra, H. Y. Huang, R. Ruoff, E. Shapiro, S. S. Taneja, S. K. Logan, and M. J. Garabedian Transcriptional Regulation of the Androgen Receptor Cofactor Androgen Receptor Trapped Clone-27 Mol. Endocrinol., December 1, 2007; 21(12): 2864 - 2876. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sun, Y. Tang, X. Lou, L. Zhu, K. Yang, B. Zhang, H. Shi, and C. Wang UXT is a novel and essential cofactor in the NF-{kappa}B transcriptional enhanceosome J. Cell Biol., July 10, 2007; 178(2): 231 - 244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-Y. Chen, G. Wulf, X. Z. Zhou, M. A. Rubin, K. P. Lu, and S. P. Balk Activation of {beta}-Catenin Signaling in Prostate Cancer by Peptidyl-Prolyl Isomerase Pin1-Mediated Abrogation of the Androgen Receptor-{beta}-Catenin Interaction Mol. Cell. Biol., February 1, 2006; 26(3): 929 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zhao, Q. Wang, H. Zhang, Q. Liu, X. Du, M. Richter, and M. I. Greene UXT Is a Novel Centrosomal Protein Essential for Cell Viability Mol. Biol. Cell, December 1, 2005; 16(12): 5857 - 5865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Li, C. N. Cavasotto, T. Cardozo, S. Ha, T. Dang, S. S. Taneja, S. K. Logan, and M. J. Garabedian Androgen Receptor Mutations Identified in Prostate Cancer and Androgen Insensitivity Syndrome Display Aberrant ART-27 Coactivator Function Mol. Endocrinol., September 1, 2005; 19(9): 2273 - 2282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Masiello, S.-Y. Chen, Y. Xu, M. C. Verhoeven, E. Choi, A. N. Hollenberg, and S. P. Balk Recruitment of {beta}-Catenin by Wild-Type or Mutant Androgen Receptors Correlates with Ligand-Stimulated Growth of Prostate Cancer Cells Mol. Endocrinol., October 1, 2004; 18(10): 2388 - 2401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Taneja, S. Ha, N. K. Swenson, I. P. Torra, S. Rome, P. D. Walden, H. Y. Huang, E. Shapiro, M. J. Garabedian, and S. K. Logan ART-27, an Androgen Receptor Coactivator Regulated in Prostate Development and Cancer J. Biol. Chem., April 2, 2004; 279(14): 13944 - 13952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, Y. Yang, S. Yeh, and C. Chang ARA67/PAT1 Functions as a Repressor To Suppress Androgen Receptor Transactivation Mol. Cell. Biol., February 1, 2004; 24(3): 1044 - 1057. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Rayala, F. Kendirgi, D. M. Barry, P. W. Majerus, and S. R. Wente The mRNA Export Factor Human Gle1 Interacts with the Nuclear Pore Complex Protein Nup155 Mol. Cell. Proteomics, February 1, 2004; 3(2): 145 - 155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Oberley, D. R. Inman, and P. J. Farnham E2F6 Negatively Regulates BRCA1 in Human Cancer Cells without Methylation of Histone H3 on Lysine 9 J. Biol. Chem., October 24, 2003; 278(43): 42466 - 42476. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Reid, I. Murray, K. Watt, R. Betney, and I. J. McEwan The Androgen Receptor Interacts with Multiple Regions of the Large Subunit of General Transcription Factor TFIIF J. Biol. Chem., October 18, 2002; 277(43): 41247 - 41253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhao, K. Goto, M. Saitoh, T. Yanase, M. Nomura, T. Okabe, R. Takayanagi, and H. Nawata Activation Function-1 Domain of Androgen Receptor Contributes to the Interaction between Subnuclear Splicing Factor Compartment and Nuclear Receptor Compartment. IDENTIFICATION OF THE p102 U5 SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE-BINDING PROTEIN AS A COACTIVATOR FOR THE RECEPTOR J. Biol. Chem., August 9, 2002; 277(33): 30031 - 30039. [Abstract] [Full Text] [PDF]
|
|
