Molecular Biology of the Cell track citations

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Originally published as MBC in Press, 10.1091/mbc.01-08-0402 on February 4, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
01-08-0402v1
13/3/817    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grissom, P. M.
Right arrow Articles by McIntosh, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grissom, P. M.
Right arrow Articles by McIntosh, J. R.

Vol. 13, Issue 3, 817-829, March 2002

Identification of a Novel Light Intermediate Chain (D2LIC) for Mammalian Cytoplasmic Dynein 2

Paula M. Grissom,* Eugeni A. Vaisberg, and J. Richard McIntosh

Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347

The diversity of dynein's functions in mammalian cells is a manifestation of both the existence of multiple dynein heavy chain isoforms and an extensive set of associated protein subunits. In this study, we have identified and characterized a novel subunit of the mammalian cytoplasmic dynein 2 complex. The sequence similarity between this 33-kDa subunit and the light intermediate chains (LICs) of cytoplasmic dynein 1 suggests that this protein is a dynein 2 LIC (D2LIC). D2LIC contains a P-loop motif near its NH2 terminus, and it shares a short region of similarity to the yeast GTPases Spg1p and Tem1p. The D2LIC subunit interacts specifically with DHC2 (or cDhc1b) in both reciprocal immunoprecipitations and sedimentation assays. The expression of D2LIC also mirrors that of DHC2 in a variety of tissues. D2LIC colocalizes with DHC2 at the Golgi apparatus throughout the cell cycle. On brefeldin A-induced Golgi fragmentation, a fraction of D2LIC redistributes to the cytoplasm, leaving behind a subset of D2LIC that is localized around the centrosome. Our results suggest that D2LIC is a bona fide subunit of cytoplasmic dynein 2 that may play a role in maintaining Golgi organization by binding cytoplasmic dynein 2 to its Golgi-associated cargo.

* Corresponding author. E-mail address: grissom{at}Colorado.edu.

Molecular Biology of the Cell
Vol. 13, 817-829, March 2002
Copyright © 2002 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
P. Rompolas, L. B. Pedersen, R. S. Patel-King, and S. M. King
Chlamydomonas FAP133 is a dynein intermediate chain associated with the retrograde intraflagellar transport motor
J. Cell Sci., October 15, 2007; 120(20): 3653 - 3665.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. A. Follit, R. A. Tuft, K. E. Fogarty, and G. J. Pazour
The Intraflagellar Transport Protein IFT20 Is Associated with the Golgi Complex and Is Required for Cilia Assembly
Mol. Biol. Cell, September 1, 2006; 17(9): 3781 - 3792.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
K. K. Pfister, E. M.C. Fisher, I. R. Gibbons, T. S. Hays, E. L.F. Holzbaur, J. R. McIntosh, M. E. Porter, T. A. Schroer, K. T. Vaughan, G. B. Witman, et al.
Cytoplasmic dynein nomenclature
J. Cell Biol., November 7, 2005; 171(3): 411 - 413.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. A. Rana, J. P. M. Barbera, T. A. Rodriguez, D. Lynch, E. Hirst, J. C. Smith, and R. S. P. Beddington
Targeted deletion of the novel cytoplasmic dynein mD2LIC disrupts the embryonic organiser, formation of the body axes and specification of ventral cell fates
Development, October 15, 2004; 131(20): 4999 - 5007.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
G. J. Pazour
Intraflagellar Transport and Cilia-Dependent Renal Disease: The Ciliary Hypothesis of Polycystic Kidney Disease
J. Am. Soc. Nephrol., October 1, 2004; 15(10): 2528 - 2536.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Hou, G. J. Pazour, and G. B. Witman
A Dynein Light Intermediate Chain, D1bLIC, Is Required for Retrograde Intraflagellar Transport
Mol. Biol. Cell, October 1, 2004; 15(10): 4382 - 4394.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. Bonnafe, M. Touka, A. AitLounis, D. Baas, E. Barras, C. Ucla, A. Moreau, F. Flamant, R. Dubruille, P. Couble, et al.
The Transcription Factor RFX3 Directs Nodal Cilium Development and Left-Right Asymmetry Specification
Mol. Cell. Biol., May 15, 2004; 24(10): 4417 - 4427.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. A. Perrone, D. Tritschler, P. Taulman, R. Bower, B. K. Yoder, and M. E. Porter
A Novel Dynein Light Intermediate Chain Colocalizes with the Retrograde Motor for Intraflagellar Transport at Sites of Axoneme Assembly in Chlamydomonas and Mammalian Cells
Mol. Biol. Cell, May 1, 2003; 14(5): 2041 - 2056.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
J. C. Schafer, C. J. Haycraft, J. H. Thomas, B. K. Yoder, and P. Swoboda
XBX-1 Encodes a Dynein Light Intermediate Chain Required for Retrograde Intraflagellar Transport and Cilia Assembly in Caenorhabditis elegans
Mol. Biol. Cell, May 1, 2003; 14(5): 2057 - 2070.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Mikami, S. H. Tynan, T. Hama, K. Luby-Phelps, T. Saito, J. E. Crandall, J. C. Besharse, and R. B. Vallee
Molecular structure of cytoplasmic dynein 2 and its distribution in neuronal and ciliated cells
J. Cell Sci., March 14, 2003; 115(24): 4801 - 4808.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]