![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 13, Issue 3, 866-879, March 2002
*Departament de Biologia Cel.lular i Anatomia Patològica,
Facultat de Medicina, Institut d'Investigacions Biomèdiques
August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, E-08036
Barcelona, Spain; Actin is involved in the organization of the Golgi complex and
Golgi-to-ER protein transport in mammalian cells. Little, however, is
known about the regulation of the Golgi-associated actin cytoskeleton. We provide evidence that Cdc42, a small GTPase that regulates actin
dynamics, controls Golgi-to-ER protein transport. We located GFP-Cdc42
in the lateral portions of Golgi cisternae and in COPI-coated and
noncoated Golgi-associated transport intermediates. Overexpression of
Cdc42 and its activated form Cdc42V12 inhibited the retrograde transport of Shiga toxin from the Golgi complex to the ER, the redistribution of the KDEL receptor, and the ER accumulation of Golgi-resident proteins induced by the active GTP-bound mutant of Sar1
(Sar1[H79G]). Coexpression of wild-type or activated Cdc42 and N-WASP
also inhibited Golgi-to-ER transport, but this was not the case in
cells expressing Cdc42V12 and N-WASP(Departamento de Biología
Celular, Facultad de Medicina, Universidad de Murcia, E-30071 Murcia,
Spain; and §Imperial Cancer Research Fund, London Wc2A
3PX, England
WA), a mutant form of N-WASP
that lacks Arp2/3 binding. Furthermore, Cdc42V12 recruited GFP-N-WASP
to the Golgi complex. We therefore conclude that Cdc42 regulates
Golgi-to-ER protein transport in an N-WASP-dependent manner.
Corresponding author. E-mail address:
egea{at}medicina.ub.es.
Present address: DPC Dipesa, Dept.
Técnico-Científico, 08029 Barcelon, Spain.
This article has been cited by other articles:
|
|
 |
|
  M. Roh-Johnson and B. Goldstein In vivo roles for Arp2/3 in cortical actin organization during C. elegans gastrulation J. Cell Sci., November 1, 2009; 122(21): 3983 - 3993. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Hehnly, K. M. Longhini, J.-L. Chen, and M. Stamnes Retrograde Shiga Toxin Trafficking Is Regulated by ARHGAP21 and Cdc42 Mol. Biol. Cell, October 15, 2009; 20(20): 4303 - 4312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. V. Egorov, M. Capestrano, O. A. Vorontsova, A. Di Pentima, A. V. Egorova, S. Mariggio, M. I. Ayala, S. Tete, J. L. Gorski, A. Luini, et al. Faciogenital Dysplasia Protein (FGD1) Regulates Export of Cargo Proteins from the Golgi Complex via Cdc42 Activation Mol. Biol. Cell, May 1, 2009; 20(9): 2413 - 2427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kodani, I. Kristensen, L. Huang, and C. Sutterlin GM130-dependent Control of Cdc42 Activity at the Golgi Regulates Centrosome Organization Mol. Biol. Cell, February 1, 2009; 20(4): 1192 - 1200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Salvarezza, S. Deborde, R. Schreiner, F. Campagne, M. M. Kessels, B. Qualmann, A. Caceres, G. Kreitzer, and E. Rodriguez-Boulan LIM Kinase 1 and Cofilin Regulate Actin Filament Population Required for Dynamin-dependent Apical Carrier Fission from the Trans-Golgi Network Mol. Biol. Cell, January 1, 2009; 20(1): 438 - 451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-E. Caruso, S. Jenna, M. Bouchecareilh, D. L. Baillie, D. Boismenu, D. Halawani, M. Latterich, and E. Chevet GTPase-Mediated Regulation of the Unfolded Protein Response in Caenorhabditis elegans Is Dependent on the AAA+ ATPase CDC-48 Mol. Cell. Biol., July 1, 2008; 28(13): 4261 - 4274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nakamura, T. Hayashi, Y. Nasu-Nishimura, F. Sakaue, Y. Morishita, T. Okabe, S. Ohwada, K. Matsuura, and T. Akiyama PX-RICS mediates ER-to-Golgi transport of the N-cadherin/{beta}-catenin complex Genes & Dev., May 1, 2008; 22(9): 1244 - 1256. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. P. Banerjee, R. Pandey, R. Zheng, M. M. Suhoski, L. Monaco-Shawver, and J. S. Orange Cdc42-interacting protein 4 functionally links actin and microtubule networks at the cytolytic NK cell immunological synapse J. Exp. Med., October 1, 2007; 204(10): 2305 - 2320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Robert, N. Smadja-Lamere, M.-C. Landry, C. Champagne, R. Petrie, N. Lamarche-Vane, H. Hosoya, and J. N. Lavoie Adenovirus E4orf4 Hijacks Rho GTPase-dependent Actin Dynamics to Kill Cells: A Role for Endosome-associated Actin Assembly Mol. Biol. Cell, July 1, 2006; 17(7): 3329 - 3344. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Xu and M. Stamnes The Actin-depolymerizing Factor Homology and Charged/Helical Domains of Drebrin and mAbp1 Direct Membrane Binding and Localization via Distinct Interactions with Actin J. Biol. Chem., April 28, 2006; 281(17): 11826 - 11833. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-L. Chen, R. V. Fucini, L. Lacomis, H. Erdjument-Bromage, P. Tempst, and M. Stamnes Coatomer-bound Cdc42 regulates dynein recruitment to COPI vesicles J. Cell Biol., May 9, 2005; 169(3): 383 - 389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Parsons, J. Monypenny, S. M. Ameer-Beg, T. H. Millard, L. M. Machesky, M. Peter, M. D. Keppler, G. Schiavo, R. Watson, J. Chernoff, et al. Spatially Distinct Binding of Cdc42 to PAK1 and N-WASP in Breast Carcinoma Cells Mol. Cell. Biol., March 1, 2005; 25(5): 1680 - 1695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Ehre, A. H. Rossi, L. H. Abdullah, K. De Pestel, S. Hill, J. C. Olsen, and C. W. Davis Barrier role of actin filaments in regulated mucin secretion from airway goblet cells Am J Physiol Cell Physiol, January 1, 2005; 288(1): C46 - C56. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jaksits, W. Bauer, E. Kriehuber, M. Zeyda, T. M. Stulnig, G. Stingl, E. Fiebiger, and D. Maurer Lipid Raft-Associated GTPase Signaling Controls Morphology and CD8+ T Cell Stimulatory Capacity of Human Dendritic Cells J. Immunol., August 1, 2004; 173(3): 1628 - 1639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kanzaki, M. Furukawa, W. Raab, and J. E. Pessin Phosphatidylinositol 4,5-Bisphosphate Regulates Adipocyte Actin Dynamics and GLUT4 Vesicle Recycling J. Biol. Chem., July 16, 2004; 279(29): 30622 - 30633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Kessels and B. Qualmann The syndapin protein family: linking membrane trafficking with the cytoskeleton J. Cell Sci., July 1, 2004; 117(15): 3077 - 3086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. T. Watson, M. Kanzaki, and J. E. Pessin Regulated Membrane Trafficking of the Insulin-Responsive Glucose Transporter 4 in Adipocytes Endocr. Rev., April 1, 2004; 25(2): 177 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Percival, J. A. I. Hughes, D. L. Brown, G. Schevzov, K. Heimann, B. Vrhovski, N. Bryce, J. L. Stow, and P. W. Gunning Targeting of a Tropomyosin Isoform to Short Microfilaments Associated with the Golgi Complex Mol. Biol. Cell, January 1, 2004; 15(1): 268 - 280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. de Toledo, F. Senic-Matuglia, J. Salamero, G. Uze, F. Comunale, P. Fort, and A. Blangy The GTP/GDP Cycling of Rho GTPase TCL Is an Essential Regulator of the Early Endocytic Pathway Mol. Biol. Cell, December 1, 2003; 14(12): 4846 - 4856. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Li, L. Blanchoin, Z. Yang, and E. M. Lord The Putative Arabidopsis Arp2/3 Complex Controls Leaf Cell Morphogenesis Plant Physiology, August 1, 2003; 132(4): 2034 - 2044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Riento, R. M. Guasch, R. Garg, B. Jin, and A. J. Ridley RhoE Binds to ROCK I and Inhibits Downstream Signaling Mol. Cell. Biol., June 15, 2003; 23(12): 4219 - 4229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Duran, F. Valderrama, S. Castel, J. Magdalena, M. Tomas, H. Hosoya, J. Renau-Piqueras, V. Malhotra, and G. Egea Myosin Motors and Not Actin Comets Are Mediators of the Actin-based Golgi-to-Endoplasmic Reticulum Protein Transport Mol. Biol. Cell, February 1, 2003; 14(2): 445 - 459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Magdalena, T. H. Millard, S. Etienne-Manneville, S. Launay, H. K. Warwick, and L. M. Machesky Involvement of the Arp2/3 Complex and Scar2 in Golgi Polarity in Scratch Wound Models Mol. Biol. Cell, February 1, 2003; 14(2): 670 - 684. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Freyberg, S. Bourgoin, and D. Shields Phospholipase D2 Is Localized to the Rims of the Golgi Apparatus in Mammalian Cells Mol. Biol. Cell, November 1, 2002; 13(11): 3930 - 3942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Chen, J. P. Ahluwalia, and M. Stamnes Selective Effects of Calcium Chelators on Anterograde and Retrograde Protein Transport in the Cell J. Biol. Chem., September 13, 2002; 277(38): 35682 - 35687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Nickel, B. Brugger, and F. T. Wieland Vesicular transport: the core machinery of COPI recruitment and budding J. Cell Sci., August 15, 2002; 115(16): 3235 - 3240. [Abstract] [Full Text] [PDF]
|
|
