![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 13, Issue 4, 1175-1189, April 2002
and§
*Mario Negri Institute for Pharmacological Research, 20157 Milan,
Italy; Previously published reports support the concept that, besides
promoting homotypic intercellular adhesion, cadherins may transfer intracellular signals. However, the signaling pathways triggered by cadherin clustering and their biological significance are still poorly understood. We report herein that transfection of VE-cadherin (VEC) cDNA in VEC null endothelial cells induces actin
rearrangement and increases the number of vinculin positive adhesion
plaques. VEC expression augments the level of active Rac but decreases active Rho. Microinjection of a dominant negative Rac mutant altered stress fiber organization, whereas inhibition of Rho was ineffective. VEC expression increased protein and mRNA levels of the Rac-specific guanosine exchange factor Tiam-1 and induced its localization at
intercellular junctions. In addition, in the presence of VEC, the
amounts of Tiam, Rac, and the Rac effector PAK as well as the level of
PAK phosphorylation were found increased in the membrane/cytoskeletal fraction. These observations are consistent with a role of VEC in
localizing Rac and its signaling partners in the same membrane compartment, facilitating their reciprocal interaction. Through this
mechanism VEC may influence the constitutive organization of the actin cytoskeleton.
Fondatione Italiana Ricerco sul Cancro (FIRC)
Institute of Molecular Oncology, 20139 Milan, Italy;
Imperial College, 5W7 2AZ London, United Kingdom; and
§Dipartimento di Scienze Cliniche e Biologiche, Facolta'
di Medicina e Chirurgia, Universita' dell'Insubria, 21100 Varese,
Italy
Corresponding author. E-mail address:
lampugnani{at}ifom-firc.it.
This article has been cited by other articles:
|
|
 |
|
  Y. Liu, D. T. Sweet, M. Irani-Tehrani, N. Maeda, and E. Tzima Shc coordinates signals from intercellular junctions and integrins to regulate flow-induced inflammation J. Cell Biol., July 14, 2008; 182(1): 185 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Dejana, F. Orsenigo, and M. G. Lampugnani The role of adherens junctions and VE-cadherin in the control of vascular permeability J. Cell Sci., July 1, 2008; 121(13): 2115 - 2122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Wheelock, Y. Shintani, M. Maeda, Y. Fukumoto, and K. R. Johnson Cadherin switching J. Cell Sci., March 15, 2008; 121(6): 727 - 735. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Vestweber VE-Cadherin: The Major Endothelial Adhesion Molecule Controlling Cellular Junctions and Blood Vessel Formation Arterioscler. Thromb. Vasc. Biol., February 1, 2008; 28(2): 223 - 232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Turowski, R. Martinelli, R. Crawford, D. Wateridge, A.-P. Papageorgiou, M. G. Lampugnani, A. C. Gamp, D. Vestweber, P. Adamson, E. Dejana, et al. Phosphorylation of vascular endothelial cadherin controls lymphocyte emigration J. Cell Sci., January 1, 2008; 121(1): 29 - 37. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. A. Komarova, D. Mehta, and A. B. Malik Dual Regulation of Endothelial Junctional Permeability Sci. Signal., November 13, 2007; 2007(412): re8 - re8. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. F. Liu, C. M. Nelson, J. L. Tan, and C. S. Chen Cadherins, RhoA, and Rac1 Are Differentially Required for Stretch-Mediated Proliferation in Endothelial Versus Smooth Muscle Cells Circ. Res., August 31, 2007; 101(5): e44 - e52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Mammoto, S. M. Parikh, A. Mammoto, D. Gallagher, B. Chan, G. Mostoslavsky, D. E. Ingber, and V. P. Sukhatme Angiopoietin-1 Requires p190 RhoGAP to Protect against Vascular Leakage in Vivo J. Biol. Chem., August 17, 2007; 282(33): 23910 - 23918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. J. Marshall Rho Family GTPase Signaling in Tumor Cell Movement and Angiogenesis Am. Assoc. Cancer Res. Educ. Book, April 14, 2007; 2007(1): 103 - 108. [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Shasby Cell-cell adhesion in lung endothelium Am J Physiol Lung Cell Mol Physiol, March 1, 2007; 292(3): L593 - L607. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kraemer, M. Goodwin, S. Verma, A. S. Yap, and R. G. Ali Rac is a dominant regulator of cadherin-directed actin assembly that is activated by adhesive ligation independently of Tiam1 Am J Physiol Cell Physiol, March 1, 2007; 292(3): C1061 - C1069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Lampugnani, F. Orsenigo, M. C. Gagliani, C. Tacchetti, and E. Dejana Vascular endothelial cadherin controls VEGFR-2 internalization and signaling from intracellular compartments J. Cell Biol., August 14, 2006; 174(4): 593 - 604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Liebner, U. Cavallaro, and E. Dejana The Multiple Languages of Endothelial Cell-to-Cell Communication Arterioscler. Thromb. Vasc. Biol., July 1, 2006; 26(7): 1431 - 1438. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zeng, S. Zhuang, J. Gloddek, C.-C. Tseng, G. R. Boss, and R. B. Pilz Regulation of cGMP-dependent Protein Kinase Expression by Rho and Kruppel-like Transcription Factor-4 J. Biol. Chem., June 23, 2006; 281(25): 16951 - 16961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Wojciak-Stothard, L. Y. F. Tsang, E. Paleolog, S. M. Hall, and S. G. Haworth Rac1 and RhoA as regulators of endothelial phenotype and barrier function in hypoxia-induced neonatal pulmonary hypertension Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1173 - L1182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Eisen, S. Walid, D. R. Ratcliffe, and G. K. Ojakian Regulation of epithelial tubule formation by Rho family GTPases Am J Physiol Cell Physiol, May 1, 2006; 290(5): C1297 - C1309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mehta and A. B. Malik Signaling Mechanisms Regulating Endothelial Permeability Physiol Rev, January 1, 2006; 86(1): 279 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Potter, S. Barbero, and D. A. Cheresh Tyrosine Phosphorylation of VE-cadherin Prevents Binding of p120- and {beta}-Catenin and Maintains the Cellular Mesenchymal State J. Biol. Chem., September 9, 2005; 280(36): 31906 - 31912. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-T. Huang, J. C. Mason, G. M. Birdsey, V. Amsellem, N. Gerwin, D. O. Haskard, A. J. Ridley, and A. M. Randi Endothelial intercellular adhesion molecule (ICAM)-2 regulates angiogenesis Blood, September 1, 2005; 106(5): 1636 - 1643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Tzima, J. S. Reader, M. Irani-Tehrani, K. L. Ewalt, M. A. Schwartz, and P. Schimmel VE-cadherin Links tRNA Synthetase Cytokine to Anti-angiogenic Function J. Biol. Chem., January 28, 2005; 280(4): 2405 - 2408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. E. Nwariaku, Z. Liu, X. Zhu, D. Nahari, C. Ingle, R. F. Wu, Y. Gu, G. Sarosi, and L. S. Terada NADPH oxidase mediates vascular endothelial cadherin phosphorylation and endothelial dysfunction Blood, November 15, 2004; 104(10): 3214 - 3220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Stockton, E. Schaefer, and M. A. Schwartz p21-activated Kinase Regulates Endothelial Permeability through Modulation of Contractility J. Biol. Chem., November 5, 2004; 279(45): 46621 - 46630. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. M. Jaffer and J. Chernoff The Cross-Rho'ds of Cell-Cell Adhesion J. Biol. Chem., August 20, 2004; 279(34): 35123 - 35126. [Full Text] [PDF]
|
|
|
|
|
|
S. Liebner, A. Cattelino, R. Gallini, N. Rudini, M. Iurlaro, S. Piccolo, and E. Dejana {beta}-Catenin is required for endothelial-mesenchymal transformation during heart cushion development in the mouse J. Cell Biol., August 2, 2004; 166(3): 359 - 367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Johnson, C. S. Theisen, K. R. Johnson, and M. J. Wheelock R-cadherin Influences Cell Motility via Rho Family GTPases J. Biol. Chem., July 23, 2004; 279(30): 31041 - 31049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bazzoni and E. Dejana Endothelial Cell-to-Cell Junctions: Molecular Organization and Role in Vascular Homeostasis Physiol Rev, July 1, 2004; 84(3): 869 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Iurlaro, F. Demontis, M. Corada, L. Zanetta, C. Drake, M. Gariboldi, S. Peiro, A. Cano, P. Navarro, A. Cattelino, et al. VE-Cadherin Expression and Clustering Maintain Low Levels of Survivin in Endothelial Cells Am. J. Pathol., July 1, 2004; 165(1): 181 - 189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Nelson, D. M. Pirone, J. L. Tan, and C. S. Chen Vascular Endothelial-Cadherin Regulates Cytoskeletal Tension, Cell Spreading, and Focal Adhesions by Stimulating RhoA Mol. Biol. Cell, June 1, 2004; 15(6): 2943 - 2953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Vincent, K. Xiao, K. M. Buckley, and A. P. Kowalczyk VE-cadherin: adhesion at arm's length Am J Physiol Cell Physiol, May 1, 2004; 286(5): C987 - C997. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Brantley-Sieders, J. Caughron, D. Hicks, A. Pozzi, J. C. Ruiz, and J. Chen EphA2 receptor tyrosine kinase regulates endothelial cell migration and vascular assembly through phosphoinositide 3-kinase-mediated Rac1 GTPase activation J. Cell Sci., April 15, 2004; 117(10): 2037 - 2049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Spagnuolo, M. Corada, F. Orsenigo, L. Zanetta, U. Deuschle, P. Sandy, C. Schneider, C. J. Drake, F. Breviario, and E. Dejana Gas1 is induced by VE-cadherin and vascular endothelial growth factor and inhibits endothelial cell apoptosis Blood, April 15, 2004; 103(8): 3005 - 3012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kouklis, M. Konstantoulaki, S. Vogel, M. Broman, and A. B. Malik Cdc42 Regulates the Restoration of Endothelial Barrier Function Circ. Res., February 6, 2004; 94(2): 159 - 166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Waschke, W. Baumgartner, R. H. Adamson, M. Zeng, K. Aktories, H. Barth, C. Wilde, F. E. Curry, and D. Drenckhahn Requirement of Rac activity for maintenance of capillary endothelial barrier properties Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H394 - H401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lee, S. Lee, A. D. Zadeh, and P. A. Kolodziej Distinct sites in E-cadherin regulate different steps in Drosophila tracheal tube fusion Development, December 15, 2003; 130(24): 5989 - 5999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cattelino, S. Liebner, R. Gallini, A. Zanetti, G. Balconi, A. Corsi, P. Bianco, H. Wolburg, R. Moore, B. Oreda, et al. The conditional inactivation of the {beta}-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility J. Cell Biol., September 15, 2003; 162(6): 1111 - 1122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Nelson and C. S. Chen VE-cadherin simultaneously stimulates and inhibits cell proliferation by altering cytoskeletal structure and tension J. Cell Sci., September 1, 2003; 116(17): 3571 - 3581. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Goodwin, E. M. Kovacs, M. A. Thoreson, A. B. Reynolds, and A. S. Yap Minimal Mutation of the Cytoplasmic Tail Inhibits the Ability of E-cadherin to Activate Rac but Not Phosphatidylinositol 3-Kinase: DIRECT EVIDENCE OF A ROLE FOR CADHERIN-ACTIVATED Rac SIGNALING IN ADHESION AND CONTACT FORMATION J. Biol. Chem., May 30, 2003; 278(23): 20533 - 20539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Lampugnani, A. Zanetti, M. Corada, T. Takahashi, G. Balconi, F. Breviario, F. Orsenigo, A. Cattelino, R. Kemler, T. O. Daniel, et al. Contact inhibition of VEGF-induced proliferation requires vascular endothelial cadherin, {beta}-catenin, and the phosphatase DEP-1/CD148 J. Cell Biol., May 26, 2003; 161(4): 793 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. K. Noren, W. T. Arthur, and K. Burridge Cadherin Engagement Inhibits RhoA via p190RhoGAP J. Biol. Chem., April 11, 2003; 278(16): 13615 - 13618. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Yap and E. M. Kovacs Direct cadherin-activated cell signaling: a view from the plasma membrane J. Cell Biol., January 2, 2003; 160(1): 11 - 16. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Corada, L. Zanetta, F. Orsenigo, F. Breviario, M. G. Lampugnani, S. Bernasconi, F. Liao, D. J. Hicklin, P. Bohlen, and E. Dejana A monoclonal antibody to vascular endothelial-cadherin inhibits tumor angiogenesis without side effects on endothelial permeability Blood, July 18, 2002; 100(3): 905 - 911. [Abstract] [Full Text] [PDF]
|
|
