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Vol. 13, Issue 4, 1203-1214, April 2002
HHMI and Department of Cell Biology, Vanderbilt University School
of Medicine, Nashville, Tennessee 37232
The Schizosaccharomyces pombe septation initiation
network (SIN) signals the onset of cell division from the spindle pole body (SPB) and is regulated by the small GTPase Spg1p. The localization of SIN components including Spg1p to the SPB is required for
cytokinesis and is dependent on Sid4p, a constitutive resident of SPBs.
However, a direct interaction between Sid4p and other members of the
SIN has not been detected. To understand how Sid4p is linked to other SIN components, we have begun to characterize an S.
pombe homolog of the Saccharomyces cerevisiae
SPB protein Nud1p. We have determined that this S. pombe
Nud1p homolog corresponds to Cdc11p, a previously uncharacterized SIN
element. We report that Cdc11p is present constitutively at SPBs and
that its function appears to be required for the localization of all
other SIN components to SPBs with the exception of Sid4p. The Cdc11p C
terminus localizes the protein to SPBs in a Sid4p-dependent manner, and
we demonstrate a direct Cdc11p-Sid4p interaction. The N-terminus of
Cdc11p is required for Spg1p binding to SPBs. Our studies indicate that
Cdc11p provides a physical link between Sid4p and the Spg1p signaling pathway.
Corresponding author. E-mail address:
kathy.gould{at}mcmail.vanderbilt.edu.
*
Both authors contributed equally to this work.
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