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Vol. 13, Issue 4, 1338-1351, April 2002
*Departments of Medicine and Biochemistry and Molecular Biology,
University of Calgary, Calgary, Alberta AB T2N 4N1, Canada;
A novel human cellular structure has been identified that contains
a unique autoimmune antigen and multiple messenger RNAs. This complex
was discovered using an autoimmune serum from a patient with motor and
sensory neuropathy and contains a protein of 182 kDa. The gene and cDNA
encoding the protein indicated an open reading frame with
glycine-tryptophan (GW) repeats and a single RNA recognition motif.
Both the patient's serum and a rabbit serum raised against the
recombinant GW protein costained discrete cytoplasmic speckles
designated as GW bodies (GWBs) that do not overlap with the Golgi
complex, endosomes, lysosomes, or peroxisomes. The mRNAs associated
with GW182 represent a clustered set of transcripts that are presumed
to reside within the GW complexes. We propose that the GW
ribonucleoprotein complex is involved in the posttranscriptional regulation of gene expression by sequestering a specific subset of gene
transcripts involved in cell growth and homeostasis.
W.M. Keck Autoimmune Disease Center, Department of
Molecular and Experimental Medicine, The Scripps Research Institute,
La Jolla, California 92037; §Department of Microbiology,
Duke University Medical Center, Durham, North Carolina 27710
Corresponding author. E-mail address:
fritzler{at}ucalgary.ca.
Alternate corresponding author. E-mail
address: echan{at}scripps.edu
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