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Originally published as MBC in Press, 10.1091/mbc.01-11-0539 on March 21, 2002
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Vol. 13, Issue 6, 1871-1880, June 2002

Centrosome Reorientation in Wound-Edge Cells Is Cell Type Specific

Anne-Marie C. Yvon,* Jonathan W. Walker,* Barbara Danowski,dagger Carey Fagerstrom,* Alexey Khodjakov,Dagger and Patricia Wadsworth*§

 *Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01002;  dagger Department of Biology, Union College, Schenectady, NY;  Dagger Wadsworth Center, N.Y. State Dept. of Health, Albany, N.Y.

The reorientation of the microtubule organizing center during cell migration into a wound in the monolayer was directly observed in living wound-edge cells expressing gamma -tubulin tagged with green fluorescent protein. Our results demonstrate that in CHO cells, the centrosome reorients to a position in front of the nucleus, toward the wound edge, whereas in PtK cells, the centrosome lags behind the nucleus during migration into the wound. In CHO cells, the average rate of centrosome motion was faster than that of the nucleus; the converse was true in PtK cells. In both cell lines, centrosome motion was stochastic, with periods of rapid motion interspersed with periods of slower motion. Centrosome reorientation in CHO cells required dynamic microtubules and cytoplasmic dynein/dynactin activity and could be prevented by altering cell-to-cell or cell-to-substrate adhesion. Microtubule marking experiments using photoactivation of caged tubulin demonstrate that microtubules are transported in the direction of cell motility in both cell lines but that in PtK cells, microtubules move individually, whereas their movement is more coherent in CHO cells. Our data demonstrate that centrosome reorientation is not required for directed migration and that diverse cells use distinct mechanisms for remodeling the microtubule array during directed migration.

§ Corresponding author. E-mail address: patw{at}bio.umass.edu.

Molecular Biology of the Cell
Vol. 13, 1871-1880, June 2002
Copyright © 2002 by The American Society for Cell Biology


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