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Vol. 13, Issue 6, 2069-2079, June 2002

Human Chromosomes 9, 12, and 15 Contain the Nucleation Sites of Stress-Induced Nuclear Bodies

Marco Denegri,^* Daniela Moralli, Mariano Rocchi,^§ Marco Biggiogera, Elena Raimondi, Fabio Cobianchi,^* Luigi De Carli, Silvano Riva,^* and Giuseppe Biamonti^*

 ^*Istituto di Genetica Molecolare del Consiglio Nazionale delle Ricerche, 27100 Pavia, Italy;  Dipartimento di Genetica e Microbiologia "A. Buzzati-Traverso," and  Dipartimento di Biologia Animale, Laboratorio di Biologia Cellulare and Centro di Studio per l'Istochimica del Consiglio Nazionale delle Ricerche, Università di Pavia, 27100 Pavia, Italy; and  ^§Sezione di Genetica-Dipartimento di Anatomia Patologica e di Genetica, 70127 Bari, Italy

We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Serine-Arginine splicing factors. In this article, we show that stress-induced SNBs are detectable in human but not in hamster cells. By means of hamster>human cell hybrids, we have identified three human chromosomes (9, 12, and 15) that are individually able to direct the formation of stress bodies in hamster cells. Similarly to stress-induced SNB, these bodies are sites of accumulation of hnRNP A1-interacting protein and heat shock factor 1, are usually associated to nucleoli, and consist of clusters of perichromatin granules. We show that the p13-q13 region of human chromosome 9 is sufficient to direct the formation of stress bodies in hamster>human cell hybrids. Fluorescence in situ hybridization experiments demonstrate that the pericentromeric heterochromatic q12 band of chromosome 9 and the centromeric regions of chromosomes 12 and 15 colocalize with stress-induced SNBs in human cells. Our data indicate that human chromosomes 9, 12, and 15 contain the nucleation sites of stress bodies in heat-shocked HeLa cells.

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Corresponding author. E-mail address: biamonti{at}igbe.pv.cnr.it.

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Molecular Biology of the Cell
Vol. 13, 2069-2079, June 2002
Copyright © 2002 by The American Society for Cell Biology

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