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Vol. 13, Issue 7, 2448-2460, July 2002
Istituto Pasteur-Fondazione Cenci Bolognetti and Centro di Genetica
Evoluzionistica del Consiglio Nazionale delle Richerche, Dipartimento
di Genetica e Biologia Molecolare, Università di Roma "La
Sapienza," 00185 Rome, Italy
We have used double-stranded RNA-mediated interference (RNAi) to
study Drosophila cytokinesis. We show that
double-stranded RNAs for anillin, acGAP,
pavarotti, rho1, pebble,
spaghetti squash, syntaxin1A, and
twinstar all disrupt cytokinesis in S2 tissue culture cells, causing gene-specific phenotypes. Our phenotypic analyses identify genes required for different aspects of cytokinesis, such as central spindle formation, actin accumulation at the cell equator, contractile ring assembly or disassembly, and membrane behavior. Moreover, the cytological phenotypes elicited by RNAi reveal
simultaneous disruption of multiple aspects of cytokinesis. These
phenotypes suggest interactions between central spindle microtubules,
the actin-based contractile ring, and the plasma membrane, and lead us
to propose that the central spindle and the contractile ring are
interdependent structures. Finally, our results indicate that RNAi in
S2 cells is a highly efficient method to detect cytokinetic genes, and
predict that genome-wide studies using this method will permit
identification of the majority of genes involved in
Drosophila mitotic cytokinesis.
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