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Originally published as MBC in Press, 10.1091/mbc.E02-02-0117 on June 6, 2002
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Vol. 13, Issue 8, 2681-2691, August 2002

Yarrowia lipolytica Cells Mutant for the PEX24 Gene Encoding a Peroxisomal Membrane Peroxin Mislocalize Peroxisomal Proteins and Accumulate Membrane Structures Containing Both Peroxisomal Matrix and Membrane Proteins

Yuen Yi C. Tam, and Richard A. Rachubinski*

Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada

Peroxins are proteins required for peroxisome assembly and are encoded by the PEX genes. Functional complementation of the oleic acid-nonutilizing strain mut1-1 of the yeast Yarrowia lipolytica has identified the novel gene, PEX24. PEX24 encodes Pex24p, a protein of 550 amino acids (61,100 Da). Pex24p is an integral membrane protein of peroxisomes that exhibits high sequence homology to two hypothetical proteins encoded by the open reading frames YHR150W and YDR479C of the Saccharomyces cerevisiae genome. Pex24p is detectable in wild-type cells grown in glucose-containing medium, and its levels are significantly increased by incubation of cells in oleic acid-containing medium, the metabolism of which requires intact peroxisomes. pex24 mutants are compromised in the targeting of both matrix and membrane proteins to peroxisomes. Although pex24 mutants fail to assemble functional peroxisomes, they do harbor membrane structures that contain subsets of peroxisomal proteins.

* Corresponding author. E-mail address: rick.rachubinski{at}ualberta.ca.

Molecular Biology of the Cell
Vol. 13, 2681-2691, August 2002
Copyright © 2002 by The American Society for Cell Biology


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