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Vol. 13, Issue 8, 2732-2746, August 2002
Department of Molecular Genetics and Microbiology, State
University of New York, Stony Brook, New York 11794-5222
The septin proteins function in the formation of septa,
mating projections, and spores in Saccharomyces
cerevisiae, as well as in cell division and other processes in
animal cells. Candida albicans septins were examined in
this study for their roles in morphogenesis of this multimorphic,
opportunistically pathogenic fungus, which can range from round budding
yeast to elongated hyphae. C. albicans green fluorescent
protein labeled septin proteins localized to a tight ring at the
bud and pseudohyphae necks and as a more diffuse array in emerging germ
tubes of hyphae. Deletion analysis demonstrated that the C.
albicans homologs of the S. cerevisiae CDC3 and
CDC12 septins are essential for viability. In contrast,
the C. albicans cdc10
and cdc11
mutants were viable but displayed conditional defects in cytokinesis,
localization of cell wall chitin, and bud morphology. The mutant
phenotypes were not identical, however, indicating that these septins
carry out distinct functions. The viable septin mutants could be
stimulated to undergo hyphal morphogenesis but formed hyphae with
abnormal curvature, and they differed from wild type in the selection
of sites for subsequent rounds of hyphal formation. The
cdc11 mutants were also defective for invasive growth
when embedded in agar. These results further extend the known roles of
the septins by demonstrating that they are essential for the proper
morphogenesis of C. albicans during both budding and
filamentous growth.
Online version of this article contains
supplemental tables. Online version available at www.molbiolcell.org.
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