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Originally published as MBC in Press, 10.1091/mbc.E02-03-0122 on June 6, 2002
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Vol. 13, Issue 8, 2771-2782, August 2002

Clastosome: A Subtype of Nuclear Body Enriched in 19S and 20S Proteasomes, Ubiquitin, and Protein Substrates of Proteasome

Miguel Lafarga,*dagger Maria Teresa Berciano,*dagger Emma Pena,* Isabel Mayo,Dagger Jose G. Castaño,Dagger Dirk Bohmann,§ João Pedro Rodrigues,|| João Paulo Tavanez,|| and Maria Carmo-Fonseca||

 *Department of Anatomy and Cell Biology, Faculty of Medicine, University of Cantabria, 39011 Santander, Spain;  Dagger Department of Biochemistry, Faculty of Medicine, Autonomous University of Madrid, 28029 Madrid, Spain;  §Center for Cancer Biology, University of Rochester Medical Center, Rochester, New York 14642; and  ||Institute of Molecular Medicine, Faculty of Medicine, University of Lisbon, Lisbon, Portugal

Nuclear bodies represent a heterogeneous class of nuclear structures. Herein, we describe that a subset of nuclear bodies is highly enriched in components of the ubiquitin-proteasome pathway of proteolysis. We coined the term clastosome (from the Greek klastos, broken and soma, body) to refer to this type of nuclear body. Clastosomes contain a high concentration of 1) ubiquitin conjugates, 2) the proteolytically active 20S core and the 19S regulatory complexes of the 26S proteasome, and 3) protein substrates of the proteasome. Although detected in a variety of cell types, clastosomes are scarce under normal conditions; however, they become more abundant when proteasomal activity is stimulated. In contrast, clastosomes disappear when cells are treated with proteasome inhibitors. Protein substrates of the proteasome that are found concentrated in clastosomes include the short-lived transcription factors c-Fos and c-Jun, adenovirus E1A proteins, and the PML protein. We propose that clastosomes are sites where proteolysis of a variety of protein substrates is taking place.

dagger These authors contributed equally to this work.

Corresponding author. E-mail address: carmo.fonseca{at}fm.ul.pt.

Molecular Biology of the Cell
Vol. 13, 2771-2782, August 2002
Copyright © 2002 by The American Society for Cell Biology


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