![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 13, Issue 9, 3042-3054, September 2002
Department of Physiology, University of Texas Health Science Center
at San Antonio, San Antonio, Texas 78229-3900
Aldosterone in some tissues increases expression of the mRNA
encoding the small monomeric G protein Ki-RasA. Renal A6
epithelial cells were used to determine whether induction of
Ki-ras leads to concomitant increases in the total as
well as active levels of Ki-RasA and whether this then leads to
subsequent activation of its effector mitogen-activated protein kinase
(MAPK/extracellular signal-regulated kinase) cascade. The
molecular basis and cellular consequences of this action were
specifically investigated. We identified the intron 1-exon 1 region
(rasI/E1) of the mouse Ki-ras gene as sufficient to
reconstitute aldosterone responsiveness to a heterologous promotor.
Aldosterone increased reporter gene activity containing rasI/E1
threefold. Aldosterone increased the absolute and GTP-bound levels of
Ki-RasA by a similar extent, suggesting that activation resulted from
mass action and not effects on GTP binding/hydrolysis rates.
Aldosterone significantly increased Ki-RasA and MAPK activity as early
as 15 min with activation peaking by 2 h and waning after 4 h. Inhibitors of transcription, translation, and a glucocorticoid
receptor antagonist attenuated MAPK signaling. Similarly,
rasI/E1-driven luciferase expression was sensitive to glucocorticoid
receptor blockade. Overexpression of dominant-negative RasN17, addition
of antisense Ki-rasA and inhibition of mitogen-activated protein kinase kinase also attenuated steroid-dependent increases in
MAPK signaling. Thus, activation of MAPK by aldosterone is dependent,
in part, on a genomic mechanism involving induction of
Ki-ras transcription and subsequent activation of its
downstream effectors. This genomic mechanism has a distinct time course
from activation by traditional mitogens, such as serum, which affect the GTP-binding state and not absolute levels of Ras. The result of
such a genomic mechanism is that peak activation of the MAPK cascade by
adrenal corticosteroids is delayed but prolonged.
This article has been cited by other articles:
|
|
 |
|
  O. Pochynyuk, J. D. Stockand, and A. Staruschenko Ion Channel Regulation by Ras, Rho, and Rab Small GTPases Experimental Biology and Medicine, November 1, 2007; 232(10): 1258 - 1265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Otani, F. Otsuka, K. Inagaki, M. Takeda, T. Miyoshi, J. Suzuki, T. Mukai, T. Ogura, and H. Makino Antagonistic effects of bone morphogenetic protein-4 and -7 on renal mesangial cell proliferation induced by aldosterone through MAPK activation Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1513 - F1525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Holzman, L. Liu, B. J. Duke, A. E. Kemendy, and D. C. Eaton Transactivation of the IGF-1R by aldosterone Am J Physiol Renal Physiol, April 1, 2007; 292(4): F1219 - F1228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Pochynyuk, J. Medina, N. Gamper, H. Genth, J. D. Stockand, and A. Staruschenko Rapid Translocation and Insertion of the Epithelial Na+ Channel in Response to RhoA Signaling J. Biol. Chem., September 8, 2006; 281(36): 26520 - 26527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. E. Brennan and P. J. Fuller Mammalian K-ras2 Is a Corticosteroid-Induced Gene in Vivo Endocrinology, June 1, 2006; 147(6): 2809 - 2816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Pochynyuk, Q. Tong, A. Staruschenko, H.-P. Ma, and J. D. Stockand Regulation of the epithelial Na+ channel (ENaC) by phosphatidylinositides Am J Physiol Renal Physiol, May 1, 2006; 290(5): F949 - F957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. N. Helms, X.-J. Chen, S. Ramosevac, D. C. Eaton, and L. Jain Dopamine regulation of amiloride-sensitive sodium channels in lung cells Am J Physiol Lung Cell Mol Physiol, April 1, 2006; 290(4): L710 - L722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Soundararajan, T. T. Zhang, J. Wang, A. Vandewalle, and D. Pearce A Novel Role for Glucocorticoid-induced Leucine Zipper Protein in Epithelial Sodium Channel-mediated Sodium Transport J. Biol. Chem., December 2, 2005; 280(48): 39970 - 39981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Staruschenko, O. Pochynyuk, and J. D. Stockand Regulation of Epithelial Na+ Channel Activity by Conserved Serine/Threonine Switches within Sorting Signals J. Biol. Chem., November 25, 2005; 280(47): 39161 - 39167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Callera, A. C. I. Montezano, A. Yogi, R. C. Tostes, Y. He, E. L. Schiffrin, and R. M. Touyz c-Src-Dependent Nongenomic Signaling Responses to Aldosterone Are Increased in Vascular Myocytes From Spontaneously Hypertensive Rats Hypertension, October 1, 2005; 46(4): 1032 - 1038. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.-J. Min, M. Mogi, J.-M. Li, J. Iwanami, M. Iwai, and M. Horiuchi Aldosterone and Angiotensin II Synergistically Induce Mitogenic Response in Vascular Smooth Muscle Cells Circ. Res., September 2, 2005; 97(5): 434 - 442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Markadieu, R. Crutzen, D. Blero, C. Erneux, and R. Beauwens Hydrogen peroxide and epidermal growth factor activate phosphatidylinositol 3-kinase and increase sodium transport in A6 cell monolayers Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1201 - F1212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Tong and J. D. Stockand Receptor tyrosine kinases mediate epithelial Na+ channel inhibition by epidermal growth factor Am J Physiol Renal Physiol, January 1, 2005; 288(1): F150 - F161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Michlig, A. Mercier, A. Doucet, L. Schild, J.-D. Horisberger, B. C. Rossier, and D. Firsov ERK1/2 Controls Na,K-ATPase Activity and Transepithelial Sodium Transport in the Principal Cell of the Cortical Collecting Duct of the Mouse Kidney J. Biol. Chem., December 3, 2004; 279(49): 51002 - 51012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Niisato, D. C. Eaton, and Y. Marunaka Involvement of cytosolic Cl- in osmoregulation of {alpha}-ENaC gene expression Am J Physiol Renal Physiol, November 1, 2004; 287(5): F932 - F939. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Staruschenko, P. Patel, Q. Tong, J. L. Medina, and J. D. Stockand Ras Activates the Epithelial Na+ Channel through Phosphoinositide 3-OH Kinase Signaling J. Biol. Chem., September 3, 2004; 279(36): 37771 - 37778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Staruschenko, J. L. Medina, P. Patel, M. S. Shapiro, R. E. Booth, and J. D. Stockand Fluorescence Resonance Energy Transfer Analysis of Subunit Stoichiometry of the Epithelial Na+ Channel J. Biol. Chem., June 25, 2004; 279(26): 27729 - 27734. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Tong, R. E. Booth, R. T. Worrell, and J. D. Stockand Regulation of Na+ transport by aldosterone: signaling convergence and cross talk between the PI3-K and MAPK1/2 cascades Am J Physiol Renal Physiol, June 1, 2004; 286(6): F1232 - F1238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Booth and J. D. Stockand Targeted degradation of ENaC in response to PKC activation of the ERK1/2 cascade Am J Physiol Renal Physiol, May 1, 2003; 284(5): F938 - F947. [Abstract] [Full Text] [PDF]
|
|
