![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 13, Issue 9, 3107-3122, September 2002
and
*Department of Molecular Medicine, National Public Health
Institute, Biomedicum Helsinki, 00251 Helsinki, Finland;
To analyze the contribution of vesicular trafficking pathways in
cellular cholesterol transport we examined the effects of selected
endosomal Rab proteins on cholesterol distribution by filipin staining.
Transient overexpression of Rab11 resulted in prominent accumulation of
free cholesterol in Rab11-positive organelles that sequestered
transferrin receptors and internalized transferrin. Sphingolipids were
selectively redistributed as pyrene-sphingomyelin and sulfatide
cosequestered with Rab11-positive endosomes, whereas globotriaosyl
ceramide and GM2 ganglioside did not. Rab11 overexpression did not
perturb the transport of
1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine-perchlorate-labeled low-density lipoprotein (LDL) to late endosomes or the Niemann-Pick type C1 (NPC1)-induced late endosomal cholesterol clearance in NPC
patient cells. However, Rab11 overexpression inhibited cellular cholesterol esterification in an LDL-independent manner. This effect
could be overcome by introducing cholesterol to the plasma membrane by
using cyclodextrin as a carrier. These results suggest that in
Rab11-overexpressing cells, deposition of cholesterol in recycling
endosomes results in its impaired esterification, presumably due to
defective recycling of cholesterol to the plasma membrane. The findings
point to the importance of the recycling endosomes in regulating
cholesterol and sphingolipid trafficking and cellular cholesterol homeostasis.
Department of Cell Biology, University Medical Center
Utrecht and Center for Biomedical Genetics, 3584 CX Utrecht, The
Netherlands; and Institute of Biomedicine, University of
Helsinki, Helsinki, Finland
This article has been cited by other articles:
|
|
 |
|
  M. Koivusalo, M. Jansen, P. Somerharju, and E. Ikonen Endocytic Trafficking of Sphingomyelin Depends on Its Acyl Chain Length Mol. Biol. Cell, December 1, 2007; 18(12): 5113 - 5123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Uzan-Gafsou, H. Bausinger, F. Proamer, S. Monier, D. Lipsker, J.-P. Cazenave, B. Goud, H. de la Salle, D. Hanau, and J. Salamero Rab11A Controls the Biogenesis of Birbeck Granules by Regulating Langerin Recycling and Stability Mol. Biol. Cell, August 1, 2007; 18(8): 3169 - 3179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Takahashi, M. Murate, M. Fukuda, S. B. Sato, A. Ohta, and T. Kobayashi Cholesterol Controls Lipid Endocytosis through Rab11 Mol. Biol. Cell, July 1, 2007; 18(7): 2667 - 2677. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Llorente-Cortes, T. Royo, M. Otero-Vinas, M. Berrozpe, and L. Badimon Sterol regulatory element binding proteins downregulate LDL receptor-related protein (LRP1) expression and LRP1-mediated aggregated LDL uptake by human macrophages Cardiovasc Res, June 1, 2007; 74(3): 526 - 536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kulinski and J. E. Vance Lipid Homeostasis and Lipoprotein Secretion in Niemann-Pick C1-deficient Hepatocytes J. Biol. Chem., January 19, 2007; 282(3): 1627 - 1637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Linder, R.-L. Uronen, M. Holtta-Vuori, P. van der Sluijs, J. Peranen, and E. Ikonen Rab8-dependent Recycling Promotes Endosomal Cholesterol Removal in Normal and Sphingolipidosis Cells Mol. Biol. Cell, January 1, 2007; 18(1): 47 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. G. Ganley and S. R. Pfeffer Cholesterol Accumulation Sequesters Rab9 and Disrupts Late Endosome Function in NPC1-deficient Cells J. Biol. Chem., June 30, 2006; 281(26): 17890 - 17899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Bradley, C. Ward, S. J. Cheng, D. L. Alexander, S. Coller, G. H. Coombs, J. D. Dunn, D. J. Ferguson, S. J. Sanderson, J. M. Wastling, et al. Proteomic Analysis of Rhoptry Organelles Reveals Many Novel Constituents for Host-Parasite Interactions in Toxoplasma gondii J. Biol. Chem., October 7, 2005; 280(40): 34245 - 34258. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Murray, M. Mavrakis, N. J. McDonald, M. Yilla, J. Sheng, W. J. Bellini, L. Zhao, J. M. Le Doux, M. W. Shaw, C.-C. Luo, et al. Rab9 GTPase Is Required for Replication of Human Immunodeficiency Virus Type 1, Filoviruses, and Measles Virus J. Virol., September 15, 2005; 79(18): 11742 - 11751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Holtta-Vuori, F. Alpy, K. Tanhuanpaa, E. Jokitalo, A.-L. Mutka, and E. Ikonen MLN64 Is Involved in Actin-mediated Dynamics of Late Endocytic Organelles Mol. Biol. Cell, August 1, 2005; 16(8): 3873 - 3886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-L. Mutka, S. Lusa, M. D. Linder, E. Jokitalo, O. Kopra, M. Jauhiainen, and E. Ikonen Secretion of Sterols and the NPC2 Protein from Primary Astrocytes J. Biol. Chem., November 19, 2004; 279(47): 48654 - 48662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Du, Y. H. Pham, and A. J. Brown Effects of 25-Hydroxycholesterol on Cholesterol Esterification and Sterol Regulatory Element-binding Protein Processing Are Dissociable: IMPLICATIONS FOR CHOLESTEROL MOVEMENT TO THE REGULATORY POOL IN THE ENDOPLASMIC RETICULUM J. Biol. Chem., November 5, 2004; 279(45): 47010 - 47016. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Choudhury, D. K. Sharma, D. L. Marks, and R. E. Pagano Elevated Endosomal Cholesterol Levels in Niemann-Pick Cells Inhibit Rab4 and Perturb Membrane Recycling Mol. Biol. Cell, October 1, 2004; 15(10): 4500 - 4511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Naslavsky, R. Weigert, and J. G. Donaldson Characterization of a Nonclathrin Endocytic Pathway: Membrane Cargo and Lipid Requirements Mol. Biol. Cell, August 1, 2004; 15(8): 3542 - 3552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Soccio and J. L. Breslow Intracellular Cholesterol Transport Arterioscler. Thromb. Vasc. Biol., July 1, 2004; 24(7): 1150 - 1160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Pasqualato, F. Senic-Matuglia, L. Renault, B. Goud, J. Salamero, and J. Cherfils The Structural GDP/GTP Cycle of Rab11 Reveals a Novel Interface Involved in the Dynamics of Recycling Endosomes J. Biol. Chem., March 19, 2004; 279(12): 11480 - 11488. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. del Pozo, N. B. Alderson, W. B. Kiosses, H.-H. Chiang, R. G. W. Anderson, and M. A. Schwartz Integrins Regulate Rac Targeting by Internalization of Membrane Domains Science, February 6, 2004; 303(5659): 839 - 842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Rzomp, L. D. Scholtes, B. J. Briggs, G. R. Whittaker, and M. A. Scidmore Rab GTPases Are Recruited to Chlamydial Inclusions in Both a Species-Dependent and Species-Independent Manner Infect. Immun., October 1, 2003; 71(10): 5855 - 5870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Heeren, T. Grewal, A. Laatsch, D. Rottke, F. Rinninger, C. Enrich, and U. Beisiegel Recycling of Apoprotein E Is Associated with Cholesterol Efflux and High Density Lipoprotein Internalization J. Biol. Chem., April 11, 2003; 278(16): 14370 - 14378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. S. Blom, M. D. Linder, K. Snow, H. Pihko, M. W. Hess, E. Jokitalo, V. Veckman, A.-C. Syvanen, and E. Ikonen Defective endocytic trafficking of NPC1 and NPC2 underlying infantile Niemann-Pick type C disease Hum. Mol. Genet., February 1, 2003; 12(3): 257 - 272. [Abstract] [Full Text] [PDF]
|
|
