Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Originally published as MBC in Press, 10.1091/mbc.E02-04-0216 on July 16, 2002
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
E02-04-0216v1
13/9/3192    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bachand, F.
Right arrow Articles by Autexier, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bachand, F.
Right arrow Articles by Autexier, C.

Vol. 13, Issue 9, 3192-3202, September 2002

The Product of the Survival of Motor Neuron (SMN) Gene is a Human Telomerase-associated Protein

François Bachand,*dagger François-Michel Boisvert,*Dagger Jocelyn Côté,*Dagger Stéphane Richard,*Dagger and Chantal Autexier*dagger §

 *Bloomfield Centre for Research in Aging, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, Montréal, Québec, H3T 1E2, Canada;  dagger Department of Anatomy and Cell Biology, McGill University, Montréal, Québec, H3A 2B2, Canada; and  Dagger Terry Fox Molecular Oncology Group and Departments of Oncology, Medicine, and Microbiology and Immunology, McGill University, Montréal, Québec, H3A 2B2, Canada

Telomerase is a ribonucleoprotein (RNP) complex that is minimally composed of a protein catalytic subunit, the telomerase reverse transcriptase (TERT), and an RNA component, the telomerase RNA. The survival of motor neuron (SMN) gene codes for a protein involved in the biogenesis of certain RNPs. Here, we report that SMN is a telomerase-associated protein. Using in vitro binding assays and immunoprecipitation experiments, we demonstrate an association between SMN and the telomerase RNP in vitro and in human cells. The specific immunopurification of SMN from human 293 cells copurified telomerase activity, suggesting that SMN associates with a subset of the functional telomerase holoenzyme. Our results also indicate that the human telomerase RNA and the human (h) TERT are not associated with Sm proteins, in contrast to Saccharomyces cerevisiae telomerase. Immunofluorescence analysis showed that hTERT does not specifically colocalize with wild-type SMN in gems or Cajal bodies. However, a dominant-negative mutant of SMN (SMNDelta N27) previously characterized to elicit the cellular reorganization of small nuclear RNPs caused the accumulation of hTERT in specific SMNDelta N27-induced cellular bodies. Furthermore, coexpression of SMNDelta N27 and hTERT in rabbit reticulocyte lysates decreased the efficiency of human telomerase reconstitution in vitro. Our results establish SMN as a novel telomerase-associated protein that is likely to function in human telomerase biogenesis.

§ Corresponding author. E-mail address: chantal.autexier{at}mcgill.ca.

Molecular Biology of the Cell
Vol. 13, 3192-3202, September 2002
Copyright © 2002 by The American Society for Cell Biology


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
C. Girard, H. Neel, E. Bertrand, and R. Bordonne
Depletion of SMN by RNA interference in HeLa cells induces defects in Cajal body formation
Nucleic Acids Res., May 31, 2006; 34(10): 2925 - 2932.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
C. Hoareau-Aveilla, M. Bonoli, M. Caizergues-Ferrer, and Y. Henry
hNaf1 is required for accumulation of human box H/ACA snoRNPs, scaRNPs, and telomerase
RNA, May 1, 2006; 12(5): 832 - 840.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
D. Fu and K. Collins
Human telomerase and Cajal body ribonucleoproteins share a unique specificity of Sm protein association
Genes & Dev., March 1, 2006; 20(5): 531 - 536.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. L. Tomlinson, T. D. Ziegler, T. Supakorndej, R. M. Terns, and M. P. Terns
Cell Cycle-regulated Trafficking of Human Telomerase to Telomeres
Mol. Biol. Cell, February 1, 2006; 17(2): 955 - 965.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Cote and S. Richard
Tudor Domains Bind Symmetrical Dimethylated Arginines
J. Biol. Chem., August 5, 2005; 280(31): 28476 - 28483.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
B. E. Jady, E. Bertrand, and T. Kiss
Human telomerase RNA and box H/ACA scaRNAs share a common Cajal body-specific localization signal
J. Cell Biol., March 1, 2004; 164(5): 647 - 652.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Zhu, R. L. Tomlinson, A. A. Lukowiak, R. M. Terns, and M. P. Terns
Telomerase RNA Accumulates in Cajal Bodies in Human Cancer Cells
Mol. Biol. Cell, January 1, 2004; 15(1): 81 - 90.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. E. Sleeman, L. Trinkle-Mulcahy, A. R. Prescott, S. C. Ogg, and A. I. Lamond
Cajal body proteins SMN and Coilin show differential dynamic behaviour in vivo
J. Cell Sci., May 15, 2003; 116(10): 2039 - 2050.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]