![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Vol. 13, Issue 9, 3257-3267, September 2002
Division of Cell Biology, The Netherlands Cancer Institute, 1066CX
Amsterdam, The Netherlands
Phosphatidylinositol 4, 5-bisphosphate (PIP2)
at the inner leaflet of the plasma membrane has been proposed to
locally regulate the actin cytoskeleton. Indeed, recent studies that
use GFP-tagged pleckstrin homology domains (GFP-PH) as fluorescent
PIP2 sensors suggest that this lipid is enriched in
membrane microdomains. Here we report that this concept needs revision.
Using three distinct fluorescent GFP-tagged pleckstrin homology
domains, we show that highly mobile GFP-PH patches colocalize perfectly
with various lipophilic membrane dyes and, hence, represent increased
lipid content rather than PIP2-enriched microdomains. We
show that bright patches are caused by submicroscopical folds and
ruffles in the membrane that can be directly visualized at ~15 nm
axial resolution with a novel numerically enhanced imaging method.
F-actin motility is inhibited significantly by agonist-induced
PIP2 breakdown, and it resumes as soon as PIP2
levels are back to normal. Thus, our data support a role for
PIP2 in the regulation of cortical actin, but they
challenge a model in which spatial differences in PIP2
regulation of the cytoskeleton exist at a micrometer scale.
Online
version of this article contains supplementary video and data
materials. Online version is available at www.molbiolcell.org.
This article has been cited by other articles:
|
|
 |
|
  U. Golebiewska, M. Nyako, W. Woturski, I. Zaitseva, and S. McLaughlin Diffusion Coefficient of Fluorescent Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane of Cells Mol. Biol. Cell, April 1, 2008; 19(4): 1663 - 1669. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. van Rheenen, X. Song, W. van Roosmalen, M. Cammer, X. Chen, V. DesMarais, S.-C. Yip, J. M. Backer, R. J. Eddy, and J. S. Condeelis EGF-induced PIP2 hydrolysis releases and activates cofilin locally in carcinoma cells J. Cell Biol., December 17, 2007; 179(6): 1247 - 1259. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Thore, A. Wuttke, and A. Tengholm Rapid Turnover of Phosphatidylinositol-4,5-Bisphosphate in Insulin-Secreting Cells Mediated by Ca2+ and the ATP-to-ADP Ratio Diabetes, March 1, 2007; 56(3): 818 - 826. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wustner Plasma Membrane Sterol Distribution Resembles the Surface Topography of Living Cells Mol. Biol. Cell, January 1, 2007; 18(1): 211 - 228. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Dowd, S. Coursol, A. L. Skirpan, T.-h. Kao, and S. Gilroy Petunia Phospholipase C1 Is Involved in Pollen Tube Growth PLANT CELL, June 1, 2006; 18(6): 1438 - 1453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Evans, D. Murray, C. C. Leslie, and J. J. Falke Specific Translocation of Protein Kinase C{alpha} to the Plasma Membrane Requires Both Ca2+ and PIP2 Recognition by Its C2 Domain Mol. Biol. Cell, January 1, 2006; 17(1): 56 - 66. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Cho, Y. A. Kim, J.-Y. Yoon, D. Lee, J. H. Kim, S. H. Lee, and W.-K. Ho Low mobility of phosphatidylinositol 4,5-bisphosphate underlies receptor specificity of Gq-mediated ion channel regulation in atrial myocytes PNAS, October 18, 2005; 102(42): 15241 - 15246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Brough, F. Bhatti, and R. F. Irvine Mobility of proteins associated with the plasma membrane by interaction with inositol lipids J. Cell Sci., July 15, 2005; 118(14): 3019 - 3025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Milosevic, J. B. Sorensen, T. Lang, M. Krauss, G. Nagy, V. Haucke, R. Jahn, and E. Neher Plasmalemmal Phosphatidylinositol-4,5-Bisphosphate Level Regulates the Releasable Vesicle Pool Size in Chromaffin Cells J. Neurosci., March 9, 2005; 25(10): 2557 - 2565. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Huang, L. Lifshitz, V. Patki-Kamath, R. Tuft, K. Fogarty, and M. P. Czech Phosphatidylinositol-4,5-Bisphosphate-Rich Plasma Membrane Patches Organize Active Zones of Endocytosis and Ruffling in Cultured Adipocytes Mol. Cell. Biol., October 15, 2004; 24(20): 9102 - 9123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Roth Phosphoinositides in Constitutive Membrane Traffic Physiol Rev, July 1, 2004; 84(3): 699 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. I. Mashanov, D. Tacon, M. Peckham, and J. E. Molloy The Spatial and Temporal Dynamics of Pleckstrin Homology Domain Binding at the Plasma Membrane Measured by Imaging Single Molecules in Live Mouse Myoblasts J. Biol. Chem., April 9, 2004; 279(15): 15274 - 15280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Price, M. Langeslag, J. P. t. Klooster, P. L. Hordijk, K. Jalink, and J. G. Collard Calcium Signaling Regulates Translocation and Activation of Rac J. Biol. Chem., October 10, 2003; 278(41): 39413 - 39421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Doughman, A. J. Firestone, M. L. Wojtasiak, M. W. Bunce, and R. A. Anderson Membrane Ruffling Requires Coordination between Type I{alpha} Phosphatidylinositol Phosphate Kinase and Rac Signaling J. Biol. Chem., June 13, 2003; 278(25): 23036 - 23045. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Xu, J. Watras, and L. M. Loew Kinetic analysis of receptor-activated phosphoinositide turnover J. Cell Biol., May 26, 2003; 161(4): 779 - 791. [Abstract] [Full Text] [PDF]
|
|
