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Vol. 13, Issue 9, 3336-3343, September 2002
Howard Hughes Medical Institute and the Department of Cell Biology,
Yale University School of Medicine, New Haven, Connecticut 06519-1418
Ypt1p regulates vesicle tethering and fusion events from the ER to
the Golgi and through the early Golgi. Genetic studies have suggested a
functional relationship between Ypt1p and Ypt31p/Ypt32p. Ypt31p and
Ypt32p are a pair of functionally redundant GTPases that act after
Ypt1p to mediate intra-Golgi traffic or the budding of post-Golgi
vesicles from the trans-Golgi. Here we report that a
novel Ypt32p exchange factor is a putative effector of Ypt1p. These
findings implicate small GTP-binding proteins of the Ypt/Rab family in
a signal cascade that directs membrane traffic through the secretory pathway.
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